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4.1.99.1: tryptophanase

This is an abbreviated version!
For detailed information about tryptophanase, go to the full flat file.

Word Map on EC 4.1.99.1

Reaction

L-tryptophan
+
H2O
=
indole
 +
pyruvate
 +
NH3

Synonyms

L-tryptophan indole-lyase, L-tryptophanase, TIL, tna2, TnaA, tnaA2, TNase, Tpase, Trpase, tryptophan indole lyase, tryptophan indole-lyase, tryptophan-indole lyase, tryptophanase, tryptophanase 2, VcTrpase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.1 tryptophanase

Storage Stability

Storage Stability on EC 4.1.99.1 - tryptophanase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 8-10 mg/ml in 0.1 M potassium phosphate buffer, pH 7.8, containing 0.1 mM pyridoxal phosphate and 1.0 mM dithiothreitol, stable for up to 1 year
-
-70°C, stored in 0.1 M potassium phosphate buffer, pH 6.8
-
5°C, stored as a suspension in 0.1 M potassium phosphate buffer, pH 8.0, containing 20% v/v glycerol, 0.1 mM pyridoxal 5'-phosphate, 10 mM mercaptoethanol and 60% saturated ammonium sulfate, stable for more than 5 months
-
stored as a suspension under nitrogen in 0.1 M potassium phosphate, pH 8.0, 0.2 mM dithiothreitol, 1 mM EDTA, and 2 M (NH4)2SO4
-