4.1.99.1: tryptophanase

This is an abbreviated version!
For detailed information about tryptophanase, go to the full flat file.

Word Map on EC 4.1.99.1

Reaction

L-tryptophan
+
H2O
=
indole
+
pyruvate
+
NH3

Synonyms

tryptophan indole-lyase, Tpase, L-tryptophanase, tryptophanase, TNase, L-tryptophan indole-lyase, Trpase, TIL, TnaA, tryptophan-indole lyase, tryptophan indole lyase, VcTrpase, tna2, tnaA2, tryptophanase 2

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.1 tryptophanase

Purification

Purification on EC 4.1.99.1 - tryptophanase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
commercial product
-
DEAE-Sephadex A-50 gel filtration or DEAE-Sepharose 4B column chromatography, and Sephacryl S-300 gel filtration
gene tnaA, recombinant enzyme from Escherichia coli strain Rosetta (DE3) pLysS by ammnoium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by preparative gel filtration, and ultrafiltration
glutathione-Sepharose column chromatography
-
hydrophobic interaction chromatography (phenyl-Sepharose)
hydrophobic interaction chromatography (Sepharose CL-aB)
-
mutant enzyme H463F
-
no added pyridoxal 5’-phosphate during purification procedure resulting in the inactive apo-form of the enzyme
recombinant enzyme from Escherichia coli strain BL21(DE3) by protamine sulfate treatment, hydrophobic interaction chromatography, and gel filtration
recombinant enzyme from Escherichia coli strain JM101
recombinant His6-tagged enzyme VcTrpase from Escherichia coli strain BL21(DE3) tn5:tnaA by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by protamine sulfate treatment, hydrophobic interaction chromatography, and gel filtration