4.1.99.1: tryptophanase

This is an abbreviated version!
For detailed information about tryptophanase, go to the full flat file.

Word Map on EC 4.1.99.1

Reaction

L-tryptophan
+
H2O
=
indole
+
pyruvate
+
NH3

Synonyms

tryptophan indole-lyase, Tpase, L-tryptophanase, tryptophanase, TNase, L-tryptophan indole-lyase, Trpase, TIL, TnaA, tryptophan-indole lyase, tryptophan indole lyase, VcTrpase, tna2, tnaA2, tryptophanase 2

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.1 tryptophanase

Cloned

Cloned on EC 4.1.99.1 - tryptophanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as glutathione S-transferase fusion protein
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) tn5:tnaA
expressed in wine yeast strains Saccharomyces cerevisiae strains YHUM272a and VIN13, expression of enzyme results in a strong increase of passion fruit aroma in wine
-
expression in Escherichia coli
-
gene tna2 or tnaA2, the transcriptional start site within the tna2 promoter is preceded by the -24 and -12 consensus sequences specified by an alternative sigma factor, sigma54 (UniProt Q67SC0), phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, coculture-dependent expression of tryptophanase in Symbiobacterium thermophilum, overview
gene tnaA
-
gene tnaA, chromosomal TnaA-sfGFP in Escherichia coli strain GL69
-
gene tnaA, expression in Escherichia coli stain BL21(DE3)
-
gene tnaA, recombinant expression of His6-tagged enzyme VcTrpase in Escherichia coli strain BL21(DE3) tn5:tnaA
gene tnaA, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)
gene tnaA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene tnaA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain GL619
gene tnaA, recombinant overexpression of the enzyme in Escherichia coli strain Rosetta (DE3) pLysS
recombinant enzyme expression in Escherichia coli strain JM101
recombinant enzyme overexpression in Escherichia coli strain SVS 370