4.1.2.40: tagatose-bisphosphate aldolase

This is an abbreviated version!
For detailed information about tagatose-bisphosphate aldolase, go to the full flat file.

Reaction

Synonyms

aldolase, tagatose 1,6-diphosphate, D-Tagatose 1,6-diphosphate aldolase, D-tagatose-1,6-bisphosphate aldolase, D-Tagatose-1,6-bisphosphate aldolase (class I), D-Tagatose-1,6-bisphosphate aldolase (class II), GatY, LacD, LacD.1, Tagatose 1,6-bisphosphate aldolase, Tagatose bisphosphate aldolase, tagatose-1,6-biphosphate aldolase 2, tagatose-1,6-bisphosphate aldolase, Tagatose-1,6-diphosphate aldolase, Tagatose-bisphosphate aldolase, TBA, TBP aldolase, TDP aldolase

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.40 tagatose-bisphosphate aldolase

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Results	in table
6549	AA Sequence
2	Application
1	CAS Registry Number
5	Cloned(Commentary)
5	Crystallization (Commentary)
1	General Stability
6	Inhibitors
6	kcat/KM [mM/s]
11	KM Value [mM]
8	Metals/Ions
8	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
25	Organism
11	Pathway
54	PDB ID
4	pH Optimum
1	pH Range
7	Protein Variants
7	Purification (Commentary)
3	Reaction
1	Reaction Type
21	Reference
3	Specific Activity [micromol/min/mg]
2	Storage Stability
41	Substrates and Products (Substrate)
9	Subunits
25	Synonyms
1	Systematic Name
6	Turnover Number [1/s]

Engineering

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Engineering on EC 4.1.2.40 - tagatose-bisphosphate aldolase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

L165E

Staphylococcus aureus

Q5HE13

the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate

715040

L165E/L275S

Staphylococcus aureus

Q5HE13

the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate

715040

L275S

Staphylococcus aureus

Q5HE13

the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate

715040

K124A

Streptococcus pyogenes

-

strongly reduced aldolase activity

679492

K204A

Streptococcus pyogenes

-

mutation results in more than 98% decrease in aldolase activity

679492

additional information

2 entries

additional information

Streptococcus pyogenes

-

construction of chimeras of isoforms LacD.1 and LacD.2. The C-terminus of LacD.1 contributes to its differential enzymatic activity, as replacing of its C-terminal half with 162 amino acid residues from the C-terminus of LacD.2 leads to an enzyme very similarly to LacD.2 in the cleavage of tagatose bisphosphate but over 3 times more efficient than LacD.2 at cleaving fructose bisphosphate. In addition, loop 1 and turn 2 influence the differential enzymatic activity of LacD.1

727768

additional information

Streptococcus pyogenes HSC5

-

construction of chimeras of isoforms LacD.1 and LacD.2. The C-terminus of LacD.1 contributes to its differential enzymatic activity, as replacing of its C-terminal half with 162 amino acid residues from the C-terminus of LacD.2 leads to an enzyme very similarly to LacD.2 in the cleavage of tagatose bisphosphate but over 3 times more efficient than LacD.2 at cleaving fructose bisphosphate. In addition, loop 1 and turn 2 influence the differential enzymatic activity of LacD.1

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