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4.1.2.22: fructose-6-phosphate phosphoketolase

This is an abbreviated version!
For detailed information about fructose-6-phosphate phosphoketolase, go to the full flat file.

Word Map on EC 4.1.2.22

Reaction

D-fructose 6-phosphate
+
phosphate
=
acetyl phosphate
+
D-erythrose 4-phosphate
+
H2O

Synonyms

All1483, All2567, F-6-ppk, F6P phosphoketolase, F6PPK, Fructose-6-phosphate phosphoketolase, Phosphoketolase, fructose 6-phosphate, X5P/F6P phosphoketolase, X5P/F6P PK, Xf2, Xfp, XFPK, Xpf, xylulose 5-phosphate/fructose 6-phosphate phosphoketolase, xylulose-5-phosphate/fructose-6-phosphate phosphoketolase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.22 fructose-6-phosphate phosphoketolase

Crystallization

Crystallization on EC 4.1.2.22 - fructose-6-phosphate phosphoketolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cryocrystallography technique, using 24% (w/v) PEG 6000, 0.1 M bicine buffer, pH 9.0
sitting drop vapor diffusion method, using 0.05 mM thiamine diphosphate, 0.25 mM MgCl2, 24% (w/v) PEG 6000 and 0.1 M Bicine pH 9.0, at 20°C
-
using 10 mg/ml thiamine diphosphate, 4% (v/v) Tacsimate pH 5.0, 12% (w/v) polyethylene glycol 3350 for the native enzyme. Se-Met substituted protein crystals are grown with 17% (w/v) polyethylene glycol 3350, 0.1 M MES (pH6.6), 0.2 M sodium thiocyanate in the absence of thiamine diphosphate
-