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4.1.2.22: fructose-6-phosphate phosphoketolase

This is an abbreviated version!
For detailed information about fructose-6-phosphate phosphoketolase, go to the full flat file.

Word Map on EC 4.1.2.22

Reaction

D-fructose 6-phosphate
+
phosphate
=
acetyl phosphate
 +
D-erythrose 4-phosphate
 +
H2O

Synonyms

All1483, All2567, F-6-ppk, F6P phosphoketolase, F6PPK, Fructose-6-phosphate phosphoketolase, Phosphoketolase, fructose 6-phosphate, X5P/F6P phosphoketolase, X5P/F6P PK, Xf2, Xfp, XFPK, Xpf, xylulose 5-phosphate/fructose 6-phosphate phosphoketolase, xylulose-5-phosphate/fructose-6-phosphate phosphoketolase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.22 fructose-6-phosphate phosphoketolase

Cloned

Cloned on EC 4.1.2.22 - fructose-6-phosphate phosphoketolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)RIL cells
expressed in Escherichia coli strain BL21 (DE3)
-
expression in Escherichia coli
Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. When phosphoketolase fome is expressed in Saccharomyces cerevisiae significant amounts of acetyl-phosphate are produced after provision of sugar phosphate substrates in vitro. Expression of bacterial phosphoketolase in Saccharomyces cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds
Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. When phosphoketolase from Bifidobacterium adolescentis is expressed in Saccharomyces cerevisiae significant amounts of acetyl-phosphate are produced after provision of sugar phosphate substrates in vitro. Expression of bacterial phosphoketolase in Saccharomyces cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds
Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. When phosphoketolase from Bifidobacterium lactis is expressed in Saccharomyces cerevisiae significant amounts of acetyl-phosphate are produced after provision of sugar phosphate substrates in vitro. Expression of bacterial phosphoketolase in Saccharomyces cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds
AJD88698.1
Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. When phosphoketolase from Clostridium acetobutylicum is expressed in Saccharomyces cerevisiae significant amounts of acetyl-phosphate are produced after provision of sugar phosphate substrates in vitro. Expression of bacterial phosphoketolase in Saccharomyces cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds
KHD36088.1
Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. When phosphoketolase from Lactobacillus plantarum is expressed in Saccharomyces cerevisiae significant amounts of acetyl-phosphate are produced after provision of sugar phosphate substrates in vitro. Expression of bacterial phosphoketolase in Saccharomyces cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds
KRU18827.1, KRU19755.1
Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. When phosphoketolase from Leuconostoc mesenteroides is expressed in Saccharomyces cerevisiae significant amounts of acetyl-phosphate are produced after provision of sugar phosphate substrates in vitro. Expression of bacterial phosphoketolase in Saccharomyces cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds
selenomethionine-labeled enzyme is expressed in Escherichia coli B834 (DE3) cells