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4.1.2.14: 2-dehydro-3-deoxy-phosphogluconate aldolase

This is an abbreviated version!
For detailed information about 2-dehydro-3-deoxy-phosphogluconate aldolase, go to the full flat file.

Word Map on EC 4.1.2.14

Reaction

2-dehydro-3-deoxy-6-phospho-D-gluconate
=
pyruvate
+
D-glyceraldehyde 3-phosphate

Synonyms

2-dehydro-3-deoxy-phosphogluconate aldolase, 2-dehydro-3-deoxyphosphogluconate aldolase, 2-keto-3-deoxy-(6-phospho)-gluconate aldolase, 2-keto-3-deoxy-6-phosphogluconate aldolase, 2-keto-3-deoxy-6-phosphogluconic aldolase, 2-keto-3-deoxygluconate-6-P-aldolase, 2-keto-3-deoxygluconate-6-phosphate aldolase, 2-oxo-3-deoxy-6-phosphogluconate aldolase, 6-phospho-2-dehydro-3-deoxy-D-gluconate D-glyceraldehyde-3-phosphate-lyase, 6-Phospho-2-keto-3-deoxygluconate aldolase, aldolase, phospho-2-keto-3-deoxygluconate, eda, KD(P)G aldolase, KDGA, KDPG aldolase, KDPG-aldolase, KDPGA, KDPGlc aldolase, ODPG aldolase, Phospho-2-dehydro-3-deoxygluconate aldolase, Phospho-2-keto-3-deoxygluconate aldolase, Phospho-2-keto-3-deoxygluconic aldolase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.14 2-dehydro-3-deoxy-phosphogluconate aldolase

Crystallization

Crystallization on EC 4.1.2.14 - 2-dehydro-3-deoxy-phosphogluconate aldolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method
sitting drop vapor diffusion method
-
microdialysis against sequentially higher concentrations of ammonium sulfate
-
structure at 2.8 A resolution
-
enzyme in complex with pyruvate, 2-dehydro-3-deoxy-D-gluconate or 2-dehydro-3-deoxy-D-galactonate, determination of the structural basis for substrate promiscuity
-
in complex with 2-dehydro-3-deoxy-6-phospho-D-gluconate
the structure of 2-keto-3-deoxy-6-phosphogluconate aldolase is solved at a resolution of 2.8 A
the crystal structure of the apoprotein is determined to a resolution of 2.0 A, the structure of the protein covalently linked to pyruvate is determined to 2.2 A
hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 3350, and 0.1 M bis-tris pH 5.5
-
apoform and in complex with 3-phosphoglycerate, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 8,000 and 0.1 M Bis-Tris (pH 5.5) for the apoform or 1.6 M ammonium sulfate, 0 .1 M CAPS (pH 10.5), and 0.2 M lithium sulfate for the 3-phosphoglycerate-bound form