4.1.1.7: benzoylformate decarboxylase
This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.
Word Map on EC 4.1.1.7
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4.1.1.7
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thiamin
-
putida
-
thdp-dependent
-
diphosphate-dependent
-
carboligation
-
carboligase
-
acyloin
-
r-benzoin
-
synthesis
-
s-mandelate
-
2-keto
-
biotechnology
- 4.1.1.7
- thiamin
- putida
-
thdp-dependent
-
diphosphate-dependent
-
carboligation
-
carboligase
- acyloin
-
r-benzoin
- synthesis
- s-mandelate
-
2-keto
- biotechnology
Reaction
Synonyms
benzoylformate decarboxylase, BFD, BfdB, BFDC, BfdM, Decarboxylase, benzoylformate, MdlC, Phenylglyoxylate decarboxylase
ECTree
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Turnover Number on EC 4.1.1.7 - benzoylformate decarboxylase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
12
3-methyl-2-oxobutanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
16
3-methyl-2-oxopentanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
4
2-ketobutanoate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
5.8
2-ketobutanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
6
2-ketobutanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
7.5
2-ketobutanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.1
2-ketohexanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.2
2-ketohexanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
4.4
2-ketohexanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
5.3
2-ketohexanoate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.81
2-ketopentanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
3.3
2-ketopentanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
6.2
2-ketopentanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
11
2-ketopentanoate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
2.7
4-methyl-2-oxopentanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
4.5
4-methylthio-2-oxobutanoate
wild type enzyme, at pH 6.0 and 30°C
4.6
4-methylthio-2-oxobutanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
20
4-methylthio-2-oxobutanoate
-
wild type enzyme, at pH 6.0 and 30°C
0.46
benzoylformate
mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
2
benzoylformate
mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
2.1
benzoylformate
mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
4.5
benzoylformate
mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
5.6
benzoylformate
mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
6.9
benzoylformate
mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
8.6
benzoylformate
mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
14
benzoylformate
mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
15
benzoylformate
mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
17
benzoylformate
mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
19
benzoylformate
mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
26
benzoylformate
mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
42
benzoylformate
mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
65
benzoylformate
mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
94
benzoylformate
mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
132
benzoylformate
mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
320
benzoylformate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
426
benzoylformate
at 30°C, in 50 mM potassium phosphate buffer, pH 6.5
1.7
benzylformate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
25
benzylformate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
110
benzylformate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
320
benzylformate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.46
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H70A
2.1
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281A
4.5
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H70F
6.9
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281Y
14
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H70L
15
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26A
26
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26M
65
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281F
132
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26L
320
phenylglyoxylate
-
pH and temperature not specified in the publication, wild-type enzyme
1.4
pyruvate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
2
pyruvate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
3.6
pyruvate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
