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4.1.1.7: benzoylformate decarboxylase

This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.7

Reaction

phenylglyoxylate
=
benzaldehyde
+
CO2

Synonyms

benzoylformate decarboxylase, BFD, BfdB, BFDC, BfdM, Decarboxylase, benzoylformate, MdlC, Phenylglyoxylate decarboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.7 benzoylformate decarboxylase

Storage Stability

Storage Stability on EC 4.1.1.7 - benzoylformate decarboxylase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, stable for 48 h
-
-20°C, lyophilized His-tagged enzyme is stable for several months
-
20°C, pH 7, in absence of cofactor and substrates, half-life is 3 days
-
20°C, pH 7, in presence of 0.5 mM thiamin diphosphate and 0.5 mM Mg2+, half-life is 36 d
-
20°C, pH 7, in presence of 0.5 mM thiamin diphosphate, 100 mM acetaldehyde and 0.5 mM Mg2+, half-life is 1.8 d
-
20°C, pH 7, in presence of 0.5 mM thiamin diphosphate, 200 mM acetaldehyde and 0.5 mM Mg2+, half-life is 1.5 d
-
20°C, pH 7, in presence of 0.5 mM thiamin diphosphate, 25 mM benzaldehyde and 0.5 mM Mg2+, half-life is 5.3 d
-
4°C, pH 7, in presence of 0.5 mM thiamin diphosphate and 0.5 mM Mg2+, half-life is 139 d
-