4.1.1.7: benzoylformate decarboxylase

This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.7

Reaction

phenylglyoxylate
=
benzaldehyde
+
CO2

Synonyms

Phenylglyoxylate decarboxylase, Decarboxylase, benzoylformate, BFD, BFDC, MdlC, BfdB, BfdM, benzoylformate decarboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.7 benzoylformate decarboxylase

Purification

Purification on EC 4.1.1.7 - benzoylformate decarboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by Ni-NTA column purification.
-
HiTrap Ni2+ chelating column chromatography
-
large-scale
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex G200 gel filtration
Phenyl Sepharose FF column chromatography and Q Sepharose FF column chromatography
-
Q-Sepharose column chromatography and Superdex 200 gel filtration
Q-Sepharose column chromatography, Superdex 200 gel filtration
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography
-
recombinant wild-type and mutant enzymes