4.1.1.7: benzoylformate decarboxylase
This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.
Word Map on EC 4.1.1.7
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4.1.1.7
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thiamin
-
putida
-
thdp-dependent
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diphosphate-dependent
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carboligation
-
carboligase
-
acyloin
-
r-benzoin
-
synthesis
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s-mandelate
-
2-keto
-
biotechnology
- 4.1.1.7
- thiamin
- putida
-
thdp-dependent
-
diphosphate-dependent
-
carboligation
-
carboligase
- acyloin
-
r-benzoin
- synthesis
- s-mandelate
-
2-keto
- biotechnology
Reaction
Synonyms
benzoylformate decarboxylase, BFD, BfdB, BFDC, BfdM, Decarboxylase, benzoylformate, MdlC, Phenylglyoxylate decarboxylase
ECTree
4.1.1.7 benzoylformate decarboxylaseAdvanced search results
results (
results (KM Value
KM Value on EC 4.1.1.7 - benzoylformate decarboxylase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.77
3-methyl-2-oxobutanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
1.2
3-methyl-2-oxopentanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
0.42
2-ketobutanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.3
2-ketobutanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
4.3
2-ketobutanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
7.5
2-ketobutanoate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.15
2-ketohexanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.28
2-ketohexanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.3
2-ketohexanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
4.1
2-ketohexanoate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.1
2-ketopentanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.28
2-ketopentanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.1
2-ketopentanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
6
2-ketopentanoate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.14
4-methyl-2-oxopentanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
0.14
4-methylthio-2-oxobutanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
1.3
4-methylthio-2-oxobutanoate
wild type enzyme, at pH 6.0 and 30°C
21
4-methylthio-2-oxobutanoate
-
wild type enzyme, at pH 6.0 and 30°C
515.9
acetaldehyde
immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C
775
acetaldehyde
free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C
19.4
benzaldehyde
free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C
51.9
benzaldehyde
immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C
0.06
benzoylformate
mutant enzyme L461V, in potassium phosphate buffer (pH 6.5, 50 mM)
0.23
benzoylformate
in 50 mM potassium phosphate buffer, pH 6.5, at 30°C
0.25
benzoylformate
mutant enzyme L461A, in potassium phosphate buffer (pH 6.5, 50 mM)
0.27
benzoylformate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.29
benzoylformate
mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.29
benzoylformate
mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.37
benzoylformate
wild type enzyme, in potassium phosphate buffer (pH 6.5, 50 mM)
0.38
benzoylformate
mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.49
benzoylformate
mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.51
benzoylformate
mutant enzyme P24A, in potassium phosphate buffer (pH 6.5, 50 mM)
0.54
benzoylformate
-
wild type enzyme, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.54
benzoylformate
mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.58
benzoylformate
-
mutant enzyme L476Q, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.59
benzoylformate
-
mutant enzyme L476G, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.6
benzoylformate
-
mutant enzyme S181T/L476P, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.8
benzoylformate
mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.85
benzoylformate
mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.85
benzoylformate
mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.89
benzoylformate
mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.9
benzoylformate
mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.94
benzoylformate
mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1
benzoylformate
-
mutant enzyme L476H, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.02
benzoylformate
-
mutant enzyme L476T, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.03
benzoylformate
-
mutant enzyme L476P, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.06
benzoylformate
-
mutant enzyme L476Q/S525G, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.08
benzoylformate
-
mutant enzyme L476M, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.14
benzoylformate
-
mutant enzyme L476S, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.17
benzoylformate
-
mutant enzyme L476A, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.2
benzoylformate
mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.2
benzoylformate
mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.23
benzoylformate
-
mutant enzyme S181T, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.26
benzoylformate
-
mutant enzyme L476C, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.4
benzoylformate
mutant enzyme L461G, in potassium phosphate buffer (pH 6.5, 50 mM)
1.46
benzoylformate
-
mutant enzyme L476K, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.5
benzoylformate
mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.54
benzoylformate
mutant enzyme A460G, in potassium phosphate buffer (pH 6.5, 50 mM)
2.9
benzoylformate
mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
7.8
benzoylformate
mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.04
benzylformate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.27
benzylformate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.45
benzylformate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.5
benzylformate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.27
phenylglyoxylate
-
pH and temperature not specified in the publication, wild-type enzyme
0.38
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H70L
0.49
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281Y
0.54
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281F
0.8
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26M
0.85
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H70F
1.2
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281A
1.2
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26L
1.5
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H70A
7.8
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26A
2
pyruvate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
4.8
pyruvate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
21
pyruvate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
additional information
additional information
KM-value of wild-type enzyme for 2-oxo-4-methylhexanoic acid (pH 6.0, 30°C) is above 20 mM
-
additional information
additional information
-
KM-value of wild-type enzyme for 2-oxo-4-methylhexanoic acid (pH 6.0, 30°C) is above 20 mM
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