4.1.1.7: benzoylformate decarboxylase
This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.
Word Map on EC 4.1.1.7
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4.1.1.7
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thiamin
-
putida
-
thdp-dependent
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diphosphate-dependent
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carboligation
-
carboligase
-
acyloin
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r-benzoin
-
synthesis
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s-mandelate
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2-keto
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biotechnology
- 4.1.1.7
- thiamin
- putida
-
thdp-dependent
-
diphosphate-dependent
-
carboligation
-
carboligase
- acyloin
-
r-benzoin
- synthesis
- s-mandelate
-
2-keto
- biotechnology
Reaction
Synonyms
benzoylformate decarboxylase, BFD, BfdB, BFDC, BfdM, Decarboxylase, benzoylformate, MdlC, Phenylglyoxylate decarboxylase
ECTree
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KCat KM Value
KCat KM Value on EC 4.1.1.7 - benzoylformate decarboxylase
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0.5
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.7
2-ketobutanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
4.6
2-ketobutanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
14
2-ketobutanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.86
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.3
2-ketohexanoate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
8
2-ketohexanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
16
2-ketohexanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.8
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
3
2-ketopentanoate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
8.1
2-ketopentanoate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
22
2-ketopentanoate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
15
3-methyl-2-oxobutanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
13
3-methyl-2-oxopentanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
20
4-methyl-2-oxopentanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
0.95
-
wild type enzyme, at pH 6.0 and 30°C
3.5
4-methylthio-2-oxobutanoate
wild type enzyme, at pH 6.0 and 30°C
33
4-methylthio-2-oxobutanoate
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
16
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
43
benzylformate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
244
benzylformate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1180
benzylformate
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.3
-
pH and temperature not specified in the publication, mutant H70A
1.7
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281A
1.9
phenylglyoxylate
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pH and temperature not specified in the publication, mutant S26A
5.3
phenylglyoxylate
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pH and temperature not specified in the publication, mutant H70F
14
phenylglyoxylate
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pH and temperature not specified in the publication, mutant H281Y
32
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26M
36
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H70L
110
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant S26L
120
phenylglyoxylate
-
pH and temperature not specified in the publication, mutant H281F
1180
phenylglyoxylate
-
pH and temperature not specified in the publication, wild-type enzyme
0.012
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.065
pyruvate
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.42
pyruvate
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.8
pyruvate
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C