4.1.1.7: benzoylformate decarboxylase

This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.

benzoylformate decarboxylase, BFD, BfdB, BFDC, BfdM, Decarboxylase, benzoylformate, MdlC, Phenylglyoxylate decarboxylase

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Results	in table
1	Activating Compound
1360	AA Sequence
6	Application
1	CAS Registry Number
23	Cloned(Commentary)
36	Cofactor
10	Crystallization (Commentary)
4	General Information
7	General Stability
32	Inhibitors
52	kcat/KM [mM/s]
14	Ki Value [mM]
134	KM Value [mM]
17	Metals/Ions
8	Molecular Weight [Da]
22	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
112	Organism
10	Pathway
96	PDB ID
13	pH Optimum
2	pH Range
6	pH Stability
96	Protein Variants
26	Purification (Commentary)
2	Reaction
7	Reaction Type
66	Reference
16	Source Tissue
7	Specific Activity [micromol/min/mg]
8	Storage Stability
250	Substrates and Products (Substrate)
13	Subunits
47	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
87	Turnover Number [1/s]

General Stability on EC 4.1.1.7 - benzoylformate decarboxylase

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BFD is stabilized by increasing water activity

significant stabilizing effect of thiamin diphosphate and Mg2+

the activity of the spherical silica-immobilized BFD A460I-F464I variant is determined to be 70% related to the activity of the free enzyme

The freeze-drying of immobilized Sepabeads EC-EA/BFD leads to an inactivation of the enzyme.

the wild type BFD has about 100% enzyme activity after 24 h incubation in 50 mM KPi-buffer without thiamine diphosphate

the wild type enzyme shows no loss of activity in cofactor-free buffer over 24 h

urea, less than 4 M, reversibly inactivates, thiamin diphosphate protects