4.1.1.7: benzoylformate decarboxylase

This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.7

Reaction

phenylglyoxylate
=
benzaldehyde
+
CO2

Synonyms

Phenylglyoxylate decarboxylase, Decarboxylase, benzoylformate, BFD, BFDC, MdlC, BfdB, BfdM, benzoylformate decarboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.7 benzoylformate decarboxylase

Crystallization

Crystallization on EC 4.1.1.7 - benzoylformate decarboxylase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 4.0 M sodium formate
-
hanging drop vapor diffusion method, structure of the enzyme complexed with the inhibitor (R)-mandelate
in complex with (E)-2-oxo-4(pyridin-3-yl)-3-butenoic acid or 3-(pyridin-3-yl)acrylaldehyde, hanging drop vapour diffusion method, in 100 mM TrisHCl (pH 8.5), 150 mM CaCl2, 0.5% (v/v) MPD [2-methyl-2,4-pentanediol], and 22% (v/v) PEG 400
mutant enzyme L461A, hanging drop vapour diffusion method
in complex with methyl benzoylphosphonate, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.5), 150 mM CaCl2, 22% (v/v) PEG 400
sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol 2000 and 0.2 M MgCl2, at pH 6.
hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.5), 150 mM CaCl2, 0.5% (w/v) 2-methyl-2,4-pentanediol, and 22% (w/v) PEG 400
mutant enzyme T377L/A460Y, hanging drop vapor diffusion method, using 0.1 M Tris (pH 8.5), 0.15 M CaCl2 and 22% PEG 400 (w/w)