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4.1.1.7: benzoylformate decarboxylase

This is an abbreviated version!
For detailed information about benzoylformate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.7

Reaction

phenylglyoxylate
=
benzaldehyde
 +
CO2

Synonyms

benzoylformate decarboxylase, BFD, BfdB, BFDC, BfdM, Decarboxylase, benzoylformate, MdlC, Phenylglyoxylate decarboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.7 benzoylformate decarboxylase

Cloned

Cloned on EC 4.1.1.7 - benzoylformate decarboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli Escherichia coli SG13009 pKK233-2
-
expressed in Escherichia coli Rosetta2(DE3)pLysS cells
-
expressed in Escherichia coli SG13009 cells
expressed in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli strain SG13009
-
expressed in Escherichia coli strain SG13009/pREP4
-
expression as a hexahistidine fusion protein in Escherichia coli
-
expression of His-tagged enzyme in Escherichia coli strain SG13009
-
expression of mutant enzyme L476Q and M365L/L461S in Escherichia coli
gene mdlC, recombinant expression in Escherichia coli strain BL21(DE3), suncloning in Escherichia coli strain DH5alpha
Production as hexahistidine fusion protein by fermentation of Escherichia coli cells as host strain.
-
recombinant expression of His-tagged enzyme in Escherichia coli strain SG13009
-
wild-type and mutant enzymes A460I, F464I and A460I/F464I