4.1.1.52: 6-methylsalicylate decarboxylase

This is an abbreviated version!
For detailed information about 6-methylsalicylate decarboxylase, go to the full flat file.

Reaction

Synonyms

6-Methylsalicylic acid (2,6-cresotic acid) decarboxylase, 6-Methylsalicylic acid decarboxylase, 6-MSA decarboxylase, AOL_s00215g281, Decarboxylase, 6-methylsalicylate, PatG

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.1 Carboxy-lyases

                4.1.1.52 6-methylsalicylate decarboxylase

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Results	in table
7	AA Sequence
2	Application
1	CAS Registry Number
4	Expression
2	General Information
1	General Stability
10	Inhibitors
5	KM Value [mM]
3	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
8	Organism
1	Oxidation Stability
1	Pathway
2	pH Optimum
1	pH Stability
1	Reaction
1	Reaction Type
8	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
2	Storage Stability
9	Substrates and Products (Substrate)
2	Subunits
12	Synonyms
1	Systematic Name
1	Temperature Stability [°C]

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Application on EC 4.1.1.52 - 6-methylsalicylate decarboxylase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

biotechnology

Aspergillus clavatus

-

biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol

757577

synthesis

Aspergillus clavatus

-

biotechnological de novo production of m-cresol from sugar in complex yeast extract-peptone medium with the yeast Saccharomyces cerevisiae. A heterologous pathway based on the decarboxylation of the polyketide 6-methylsalicylic acid is introduced into a CEN.PK yeast strain. Overexpression of codon-optimized 6-methylsalicylic acid synthase from Penicillium patulum together with activating phosphopantetheinyl transferase npgA from Aspergillus nidulans results in up to 367 mg/l 6-methylsalicylic acid production. Additional genomic integration of the genes have a strongly promoting effect and 6-methylsalicylic acid titers reach more than 2 g/l. Simultaneous expression of 6-methylsalicylic acid decarboxylase patG from Aspergillus clavatus leads to the complete conversion of 6-methylsalicylic acid and production of up to 589 mg/L m-cresol

757577