4.1.1.47: tartronate-semialdehyde synthase

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For detailed information about tartronate-semialdehyde synthase, go to the full flat file.

Reaction

2 glyoxylate =

Synonyms

GCL, Glyoxalate carboligase, Glyoxylate carbo-ligase, Glyoxylate carboligase, Glyoxylate carboxy-lyase, Glyoxylate carboxy-lyase (dimerizing and reducing), Glyoxylic carbo-ligase, Hydroxymalonic semialdehyde carboxylase, Synthase, tartronate semialdehyde, Tartronate semialdehyde carboxylase, tartronate semialdehyde synthase, Tartronate-semialdehyde synthase, Tartronic semialdehyde carboxylase, Tartronic semialdehyde synthase

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.1 Carboxy-lyases

                4.1.1.47 tartronate-semialdehyde synthase

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Results	in table
3379	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
11	Cofactor
1	Crystallization (Commentary)
2	Expression
2	General Information
11	kcat/KM [mM/s]
6	KM Value [mM]
1	Localization
2	Metals/Ions
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
63	Organism
5	Pathway
6	PDB ID
1	pH Optimum
12	Protein Variants
4	Purification (Commentary)
1	Reaction
2	Reaction Type
21	Reference
11	Specific Activity [micromol/min/mg]
27	Substrates and Products (Substrate)
4	Subunits
28	Synonyms
1	Systematic Name
5	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.1.1.47 - tartronate-semialdehyde synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

glyoxylate carboligase crystallizes with 6 monomers (a tetramer and a dimer) in an asymmetric unit, vapor diffusion hanging-drop, 2-4 microlitre of protein solution (5-15 mg/ml, 100 micromolar ThDP, 10 micromolar FAD, 1mM MgCl2 and 10 mM quinone Q0) are mixed with equal volume of reservoir solution (0.5% PEG6000, 0.5M NaCl, 40 mM DTT), pH 8.00, temperature 294K, space group P41212, resolution 2.70 A

Escherichia coli K-12

P0AEP7

705846