4.1.1.33: diphosphomevalonate decarboxylase

This is an abbreviated version!
For detailed information about diphosphomevalonate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.33

Reaction

ATP
+
(R)-5-diphosphomevalonate
=
ADP
+
phosphate
+
isopentenyl diphosphate
+
CO2

Synonyms

More, Decarboxylase, pyrophosphomevalonate, Pyrophosphomevalonate decarboxylase, Mevalonate-5-pyrophosphate decarboxylase, Pyrophosphomevalonic acid decarboxylase, 5-Pyrophosphomevalonate decarboxylase, Mevalonate 5-diphosphate decarboxylase, Mevalonate (diphospho)decarboxylase, Mevalonate pyrophosphate decarboxylase, MDP, mevalonate pyrophosphate decraboxylase, mevalonate diphosphate decarboxylase, MVD, MPD, MDD, mevalonate 5'-diphosphate decarboxylase, diphosphomevalonate decarboxylase, DPM-DC, scMVD, mevalonate diphospho decarboxylase, ScMDD, ScMDD1, ScMDD2, phosphomevalonate decarboxylase, PMD, SSO2989, DMD, MvaD, diphosphomevalonte decarboxylase, Fjoh_1389

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.33 diphosphomevalonate decarboxylase

Engineering

Engineering on EC 4.1.1.33 - diphosphomevalonate decarboxylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S162A
mutant shows reduced activity with 2fold decrease in Vm and 4 to 7fold increases in substrate Km values
R161Q
mutant exhibits an about 1000fold diminution in specific activity, while binding the fluorescent substrate analog, TNP-ATP, comparably to wild type enzyme
N17A
mutant exhibits a 15fold inflation in Km for 5-diphosphomevalonate
K27A
-
low activity
D306A
-
low activity
R154A
-
low activity
D306N
-
low activity
K23A
-
no activity
R162A
-
low activity
K208A
-
low activity
D306E
-
low activity
C210S
decrease in melting temperature by 12 degrees
D281N
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity and phosphate elimination/decarboxylation
D281T
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
D281V
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
D281N
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity and phosphate elimination/decarboxylation
-
D281T
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
D281V
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
C210S
-
decrease in melting temperature by 12 degrees
-
D281N
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity and phosphate elimination/decarboxylation
-
D281T
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
D281V
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
D281N
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity and phosphate elimination/decarboxylation
-
D281T
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
D281V
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
D281N
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity and phosphate elimination/decarboxylation
-
D281T
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
D281V
-
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation
-
S120A
8fold diminution in kcat-value
D302N
100000fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site
S153A
18fold inflation in Kd-value for Mg-ATP, 35fold inflation in Km-value for Mg-ATP
S36A
limited solubility, impaired binding of fluorescent ATP analogue trinitrophenyl-ATP
S155A
26fold inflation in Km-value for Mg-ATP, 16fold inflation in Km-value for diphosphomevalonate
D302A
1000fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site
K18M
30fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site
S121A
42000fold diminution in kcat-value
R74H
mutant increases specific activity on non-native substrate (3-hydroxy-3-methylbutyrate) by 4.8fold
D283A
W153F
modest effects on kinetics
S192A
W19F
130fold increase in Km for (R)-5-diphosphomevalonate, 10fold increase in Km for ATP
R193T
modest effects on kinetics
additional information