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4.1.1.31: phosphoenolpyruvate carboxylase

This is an abbreviated version!
For detailed information about phosphoenolpyruvate carboxylase, go to the full flat file.

Word Map on EC 4.1.1.31

Reaction

phosphate
+
oxaloacetate
=
phosphoenolpyruvate
 +
HCO3-

Synonyms

archaeal-type phosphoenolpyruvate carboxylase, AT1G53310, atPEPC, Atppc1, Atppc2, Atppc3, Atppc4, bacterial-type PEPC, bacterial-type phosphoenolpyruvate carboxylase, BPTC, BTPC, C4 PEPC, C4-PEPC, Carboxylase, phosphopyruvate (phosphate), Class-1 PEPC, Class-2 PEPC, CP21, CP28, CP46, CrPpc1, CrPpc2, Hvpepc3, Hvpepc4, Hvpepc5, Ljpepc1, NCgl1523, OJ1484_G09.129-1, Osppc2a, Osppc4, PEOC, PEP carboxylase, PEP-carboxylase, PEPC, PEPC(p102)kinase, PEPC1, PEPC2, PEPC3, PEPC7, PepcA, PEPCase, PEPCase1, PEPCK, PEPK, phosphoenol pyruvate carboxylase, phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase, Phosphoenolpyruvic carboxylase, photosynthetic phosphoenolpyruvate carboxylase, plant-type phosphoenolpyruvate carboxylase, PPC, PPC1, PPC2, PPC2a, PPC3, PPC4, PpcA, PpcA1, PPCK1, PPCK2, PTPC, S.minutum PEPC1, Sb02g021090, Sb04g008720, SORBI_3007G106500, SSO2256, SvPEPC

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.31 phosphoenolpyruvate carboxylase

Storage Stability

Storage Stability on EC 4.1.1.31 - phosphoenolpyruvate carboxylase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-12°C, 0.4 M potassium phosphate buffer containing 0.5 M glucose, 1 mM EDTA, 1 mM MgCl2, no loss of activity after 3 months
-
-14°C, isoenzyme I loses 50% of its activity in presence of ethanol
-
-16°C, 10% glycerol, 1% bovine serum albumin, stable for up to 1 year
-
-18°C, 10 mM 2-mercaptoethanol, stable for several weeks
-
-18°C, 20% sucrose solution, stable for 14 days
-
-20°C, 0.04 M Tris/HCl buffer, pH 7.8, 2.0 mM dithiothreitol, 2.0 mM MgCl2, 1.0 mM EDTA, 20% glycerol, 1.0 mM Pro, stable for at least 4 weeks
-
-20°C, 50% (v/v) glycerol, the final PPC4 preparation proves difficult to store as it slowly loses activity and is completely inactivated when rapidly thawed after freezing in liquid N2
-20°C, little loss of activity after 8 months
-
-20°C, stable for several weeks
-
-4°C or -20°C, marked decrease in activity after 2 months
-
-80°C, concentrated, undesalted solution containing 33% v/v glycerol, stable for at least 6 weeks
Coccochloris peniocystis
-
-80°C, stable for at least 1 month
-
0-4°C, stable for 3 d
-
4°C, in (NH4)2SO4 suspension, 1 month stable
-
4°C, in 50% ammonium sulfate, stable for 2-3 weeks
-
in liquid N2 in presence of 50% v/v glycerol, up to 3 months the enzyme largely retains its full activity
-