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4.1.1.31: phosphoenolpyruvate carboxylase

This is an abbreviated version!
For detailed information about phosphoenolpyruvate carboxylase, go to the full flat file.

Word Map on EC 4.1.1.31

Reaction

phosphate
+
oxaloacetate
=
phosphoenolpyruvate
 +
HCO3-

Synonyms

archaeal-type phosphoenolpyruvate carboxylase, AT1G53310, atPEPC, Atppc1, Atppc2, Atppc3, Atppc4, bacterial-type PEPC, bacterial-type phosphoenolpyruvate carboxylase, BPTC, BTPC, C4 PEPC, C4-PEPC, Carboxylase, phosphopyruvate (phosphate), Class-1 PEPC, Class-2 PEPC, CP21, CP28, CP46, CrPpc1, CrPpc2, Hvpepc3, Hvpepc4, Hvpepc5, Ljpepc1, NCgl1523, OJ1484_G09.129-1, Osppc2a, Osppc4, PEOC, PEP carboxylase, PEP-carboxylase, PEPC, PEPC(p102)kinase, PEPC1, PEPC2, PEPC3, PEPC7, PepcA, PEPCase, PEPCase1, PEPCK, PEPK, phosphoenol pyruvate carboxylase, phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase, Phosphoenolpyruvic carboxylase, photosynthetic phosphoenolpyruvate carboxylase, plant-type phosphoenolpyruvate carboxylase, PPC, PPC1, PPC2, PPC2a, PPC3, PPC4, PpcA, PpcA1, PPCK1, PPCK2, PTPC, S.minutum PEPC1, Sb02g021090, Sb04g008720, SORBI_3007G106500, SSO2256, SvPEPC

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.31 phosphoenolpyruvate carboxylase

Cloned

Cloned on EC 4.1.1.31 - phosphoenolpyruvate carboxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
carbon metabolism is analysed in generated transgenic rice plants expressing either PEPC or both phosphoenolpyruvate carboxykinase (PCK) and PEPC: Results suggest that overexpression of PEPC enhances the anaplerotic pathway rather than the initial carbon fixation of the C4-like photosynthetic pathway, and that elevated PEPC activity in combination with PCK activity contributes little to C4-like carbon flow
-
cloned as a Histag-fusion protein in an Escherichia coli PEPC- (Ppc-) strain
cloned in Escherichia coli DH5alpha. Knock-out as well as over-expression mutants are constructed and characterized. Knocking out phosphoenolpyruvate carboxylase decreases the maximum cell density by 14% and increases the acetate excretion by 7%. Over-expression of phosphoenolpyruvate carboxylase increases the maximum cell dry weight by 91%. No acetate excretion is detected at these increased cell densities
-
cloning of ppc1 in Escherichia coli
cloning of ppc2 in Escherichia coli
codon-optimized expression in Escherichia coli
WP_019935846
expressed in Arabidopsis thaliana
-
expressed in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter. SvPEPC is capable of efficiently exerting its activity in the plant cell environment so as to cause imbalance between aromatic and non-aromatic amino acid synthesis
Thermostichus vulcanus
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) pLysS cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-RIL cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells
-
expressed in Escherichia coli SGJS117
-
expressed in Escherichia coli strains BL21(DE) and BL21-Gold(DE)
expressed in Escherichia coli with an N-terminal His-tag
expressed in Nicotiana tabacum BY-2 cells
-
expressed in Sinorhizobium meliloti mutant RmF991
-
expression in Escherichia coli
expression in Escherichia coli as a fusion protein, the extra 159 amino acid residues fused at the N-terminus of the enzyme protein have no effect on catalytic and regulatory properties
-
expression in Escherichia coli as a fusion with the Escherichia coli maltose-binding protein
expression in Escherichia coli BL21(DE3)pLysS
-
high level of PEPC gene is stably inherited and transferred from the male parent, PEPC transgenic rice, into a female parent, japonica rice cv. 9516. The produced JAAS45 pollen lines are more tolerant to photoinhibition and to photo-oxidative stress.
-
isozymic forms are encoded by a small gene family
-
phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase are overexpressed in Escherichia coli concurrently to improve the production of succinate. This coexpression system is also applied to mutant strains of Escherichia coli strategically designed by inactivating the competing pathways of succinate formation. Coexpression of phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase is effective in depleting pyruvate accumulation and increasing the production of metabolites
-
phosphoenolpyruvate carboxylase is ectopically overexpressed in Vicia narbonensis seeds: Transgenic embryos take up more carbon and nitrogen. Changes in dry to FW ratio, seed fill duration and major seed components indicate altered seed development. Array-based gene expression analysis of embryos reveals upregulation of seed metabolism, especially during the transition phase and at late maturation.
-
root enzyme
-
subcloned into the streptomycete high-copy-number plasmid vector pIJ486 and transferred into Streptomyces lividans
-
to elucidate the photosynthetic physiological characteristics and the physiological inherited traits of rice hybrids and their parents, physiological indices of photosynthetic CO2 exchange and chlorophyll fluorescence parameters are measured in leaves of the maize phosphoenolpyruvate carboxylase (PEPC) transgenic rice as the male parent, sp. japonica rice cv. 9516 as the female parent, and the stable JAAS45 pollen line. The results reveal that the PEPC gene can be stably inherited and transferred from the male parent to the JAAS45 pollen line
-
using the antisense technique the expression of Ljpepc1 is decreased in three independent transgenic Lotus japonicus plants (designated as Asppc1, Asppc2 and Asppc3). In nodules of Asppc plants, PEPC activity is reduced to about 10% of that of non-transformants and the plants show typical nitrogen-deficient symptoms without a supply of nitrogen nutrient, and returned to normal growth when nitrate was supplied at 2.5 mM. The acetylene reduction activity per fresh weight of nodules of these Asppc plants decreases by 29% at 35 days after infection
-