4.1.1.23: orotidine-5'-phosphate decarboxylase

This is an abbreviated version!
For detailed information about orotidine-5'-phosphate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.23

Reaction

orotidine 5'-phosphate
=
UMP
+
CO2

Synonyms

More, Decarboxylase, orotidine 5'-phosphate, OMP decarboxylase, OMP-DC, Orotate monophosphate decarboxylase, Orotate decarboxylase, Orotic decarboxylase, Orotidine-5'-monophosphate decarboxylase, Orotidine phosphate decarboxylase, Orotidine 5'-monophosphate decarboxylase, Orotidine monophosphate decarboxylase, Orotidine 5'-phosphate decarboxylase, Orotidylate decarboxylase, Orotidylic decarboxylase, Orotidylic acid decarboxylase, Orotodylate decarboxylase, ODCase, OMPDCase, OMPdecase, UMP synthase, Uridine 5'-monophosphate synthase, OMPDC, OMPD, PfOMPDC, orotidine-5'-phosphate decarboxylase, PbrURA3, orotidine-5-monophosphate decarboxylase, orotidine 5-monophosphate decarboxylase, orotidine 5-phosphate decarboxylase, PfODCase, PyrF, URA3, ScOMPDC, pm-ura3

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.23 orotidine-5'-phosphate decarboxylase

Storage Stability

Storage Stability on EC 4.1.1.23 - orotidine-5'-phosphate decarboxylase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, storage of the high molecular weight forms in presence of 0.15 mM oxipurinol prevents the spontaneous dissociation which occures when these forms are stored in 50 mM potassium phosphate, pH 7.4
-
-20°C, 20% glycerol, retention of full activity for more than 2 years
-
-25°C, stable
-
-70°C, dilute buffer plus 2 mM dithiothreitol, purified UMP synthase activity retains orotidine-5'-phosphate decarboxylase activity for several months
-
0-4°C, stable in 10 mM potassium phosphate buffer, pH 5.8-7.5, containing 5 mM 2-mercaptoethanol for several weeks
-
0°C or -70°C, 5 mM potassium phosphate, pH 7.0, 2 mM dithiothreitol, rapid loss of orotidine-5'-phosphate decarboxylase activity of the orotidine-5'-phosphate decarboxylase domain of UMP synthase
-
4°C, purified recombinant bifunctional enzyme, stability property of both domains in the bifunctional enzyme in the absence or presence of 0.5 mM PRPP and 0.5 mM UMP: half-life of the OPRT activity is about 3-4 days and 3 weeks, respectively. The OMPDC activity is much more stable with half-life of about 3-4 weeks. 90% of the bifunctional protein, stored for 4 weeks, remains as a bifunctional protein band in SDS-PAGE and image analyses
4°C, stable for 1 week
-
4°C, stable for 16 months
-