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4.1.1.23: orotidine-5'-phosphate decarboxylase

This is an abbreviated version!
For detailed information about orotidine-5'-phosphate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.23

Reaction

orotidine 5'-phosphate
=
UMP
 +
CO2

Synonyms

Decarboxylase, orotidine 5'-phosphate, More, ODCase, OMP decarboxylase, OMP-DC, OMPD, OMPDC, OMPDCase, OMPdecase, Orotate decarboxylase, Orotate monophosphate decarboxylase, Orotic decarboxylase, Orotidine 5'-monophosphate decarboxylase, Orotidine 5'-phosphate decarboxylase, orotidine 5-monophosphate decarboxylase, orotidine 5-phosphate decarboxylase, Orotidine monophosphate decarboxylase, Orotidine phosphate decarboxylase, Orotidine-5'-monophosphate decarboxylase, orotidine-5'-phosphate decarboxylase, orotidine-5-monophosphate decarboxylase, Orotidylate decarboxylase, Orotidylic acid decarboxylase, Orotidylic decarboxylase, Orotodylate decarboxylase, PbrURA3, PfODCase, PfOMPDC, pm-ura3, PyrF, ScOMPDC, UMP synthase, URA3, Uridine 5'-monophosphate synthase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.23 orotidine-5'-phosphate decarboxylase

Purification

Purification on EC 4.1.1.23 - orotidine-5'-phosphate decarboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
functional recombinant enzyme 300fold from Escherichia coli by nickel affinity chromatography and gel filtration
-
partial
-
preparation and isolation of the orotidine-5'-phosphate decarboxylase domain of UMP synthase
-
recombinant enzyme
recombinant His-tagged chimeric fusion enzyme from Escherichia coli by nickel affinity and anion exchange chromatography to near homogeneity
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, tag cleavage with thrombin, and gel filtration
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant ODCase expressed in Escherichia coli
-
recombinant wild-type and mutant enzyme
-
using a Ni and a gel filtration column, the His6-tag is removed by thrombin digestion
-
using a Ni-NTA agarose column, the polyhistidine tag is removed with thrombin, the cleaved protein is further purified using a POROS-HS cation exchange and a Superdex-200 size-exclusion column
using a Ni2+-nitrilotriacetic acid-agarose affinity and a Superdex-75 column
-
using Affi-Gel Blue chromatography
-