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3.7.1.1: oxaloacetase

This is an abbreviated version!
For detailed information about oxaloacetase, go to the full flat file.

Word Map on EC 3.7.1.1

Reaction

oxaloacetate
+
H2O
=
oxalate
+
acetate

Synonyms

(2R,3S)-dimethylmalate lyase, An07g08390, BbOAH, DMML, FpOAH, OAH, OAH-active petal death protein, OAH-like protein, OXA, oxalacetase, oxalacetate acetylhydrolase, oxalacetic hydrolase, oxaloacetase, oxaloacetate acethylhydrolase, oxaloacetate acetylhydrolase, oxaloacetate hydrolase

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.1 oxaloacetase

Cloned

Cloned on EC 3.7.1.1 - oxaloacetase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned by inverse PCR into Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
gene An07g08390, DNA and amino acid sequence determination and analysis
gene An15g07720, DNA and amino acid sequence determination and analysis
gene gene Ss-oah1, DNA and amino acid sequence determination and analysis, PCR-based genome walking
gene oah, cloned from strain WU-2223L, recombinant overexpression in Aspergillus strain WU-2223L, construction of plasmids pDOAHPTR-1 and pNANOAH-1 for enzyme overexpression in strainWU-2223L
gene oahA, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3)
gene Pc22g24830, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, functional expression in Saccharomyces cerevisiae strain IME043, IME046, and IME047
-
into the vector pET-3c
into the vector pGEM-T Easy for sequencing
into the vector pGEM-T Easy for sequencing, and into pET-3c for expression in Escherichia coli BL21DE3 cells
OA-producing strain EOAH-1, generated by overexpression of the oxaloacetate hydrolase gene oahA in Aspergillus niger WU-2223L, produces 28 g/l oxalate from 30 g/l glucose during the 9-day cultivation period
-