3.6.4.6: vesicle-fusing ATPase

This is an abbreviated version!
For detailed information about vesicle-fusing ATPase, go to the full flat file.

Reaction

Synonyms

AAA ATPase Vps4, AAA-ATPase, ATP phosphohydrolase, ATP6V0D1, ATP6V0D2, ATPase, ATPase N-ethylmaleimide sensitive factor, C06A1.1, C41C4.8, CDC-48.1, CDC-48.2, Cdc48, Cdc48p, Csc1p, Did6p, dNSF, dNSF-1, dNSF1, End13p, Grd13p, Helar-NSF, Hez-NSF, HsPEX1, HsPex1p, HsPex6p, membrane deformation AAA ATPase, N-ethyl maleimide sensitive factor, N-ethylmaleimide sensitive factor, N-ethylmaleimide sensitive fusion protein, N-ethylmaleimide sensitive-factor, N-ethylmaleimide-sensitive factor, N-ethylmaleimide-sensitive fusion protein, N-ethylmeleimide-sensitive factor, NEM-sensitive fusion protein, NSF, NSF ATPase, NSF protein, NSF-1, NSF2, nsfA, NSFII, p97, p97/VCP/Cdc48p, p97/VCP/Cdc48p homologue, p97ATPase, peptide p97, Pex1, Pex1p, pex22, Pex6p, PpPex1p, PpPex6p, rat Pex6p, Sec18, SEC18 gene product, SEC18p, SKD1, SKD1/VPS4B, SKD2, SKD2 protein, Sso0909, SsoVps4, suppresor of potassium transport growth defect, Suppressor of K+ Transport Growth Defect1, triple-A ATPase, V-ATPase, vacular protein sorting 4 B, vacuolar protein sorting 4, vacuolar protein sorting 4 protein, vasolin-containing protein, VCP, vesicle-fusing ATPase, Vesicular-fusion protein NSF, Vpl4p, Vps 4B, Vps4, Vps4 ATPase, VPS4 gene product, Vps4p, Vps4p AAA ATPase, Vpt10p

ECTree

     3 Hydrolases

         3.6 Acting on acid anhydrides

             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement

                3.6.4.6 vesicle-fusing ATPase

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Results	in table
19	Activating Compound
11969	AA Sequence
8	Application
1	CAS Registry Number
30	Cloned(Commentary)
2	Cofactor
11	Crystallization (Commentary)
469	Disease/ Diagnostics
1	Expression
29	General Information
7	Inhibitors
2	KM Value [mM]
66	Localization
16	Metals/Ions
22	Molecular Weight [Da]
93	Natural Substrates/ Products (Substrates)
177	Organism
562	PDB ID
6	pH Optimum
1	pH Range
3	Posttranslational Modification
54	Protein Variants
23	Purification (Commentary)
35	Reaction
19	Reaction Type
104	Reference
51	Source Tissue
5	Specific Activity [micromol/min/mg]
109	Substrates and Products (Substrate)
35	Subunits
200	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 3.6.4.6 - vesicle-fusing ATPase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

ATP + H2O

ADP + phosphate

42 entries

additional information

?

-

5 entries

ATP + H2O

ADP + phosphate

Arabidopsis thaliana

-

-

688690, 689461

-

-

ir

ATP + H2O

ADP + phosphate

Arabidopsis thaliana

Q9ZNT0

-

734975

-

-

?

ATP + H2O

ADP + phosphate

Archaea

-

-

688690

-

-

ir

ATP + H2O

ADP + phosphate

Bos taurus

-

ATPase N-ethylmaleimide sensitive factor may be a common feature in the triggering/regulation of membrane merging during mammalian acrosome reaction

667294

-

-

?

ATP + H2O

ADP + phosphate

Caenorhabditis elegans

-

the VCP/Cdc48p homologues C41C4.8 and C06A1.1, AAA chaperones, may play a protective role in polyglutamine aggregation

670042

-

-

?

ATP + H2O

ADP + phosphate

Candida albicans

-

-

210576

-

-

?

ATP + H2O

ADP + phosphate

Cricetulus griseus

-

-

656763, 734226

-

-

?

ATP + H2O

ADP + phosphate

Cricetulus longicaudatus

-

-

210576

-

-

?

ATP + H2O

ADP + phosphate

Danio rerio

-

essential requirement for NSF in the organization of myelinated axons

668336

-

-

?

ATP + H2O

ADP + phosphate

Dictyostelium discoideum

Q75JI3

-

756637

-

-

?

ATP + H2O

ADP + phosphate

Drosophila melanogaster

-

-

210576, 656585, 657180

-

-

?

ATP + H2O

ADP + phosphate

Drosophila melanogaster

P46461

-

686724

-

-

ir

ATP + H2O

ADP + phosphate

Drosophila melanogaster

-

-

688690

-

-

ir

ATP + H2O

ADP + phosphate

Drosophila melanogaster

P54351

-

210579

-

-

?

ATP + H2O

ADP + phosphate

Drosophila melanogaster

Q9Y162

-

733702

-

-

?

ATP + H2O

ADP + phosphate

Drosophila sp. (in: flies)

-

NSF is important in vesicular trafficking, but neural expression of a dominant negative form of NSF2 induces an unexpected overgrowth of the DRosophila larval neuromuscular synapse

668742

-

-

?

ATP + H2O

ADP + phosphate

Helicoverpa armigera

-

-

689343

-

-

ir

ATP + H2O

ADP + phosphate

Helicoverpa armigera

-

a central function of Helar-NSF in developmental process through regulating neurohormonr release

668945

-

-

?

ATP + H2O

ADP + phosphate

Helicoverpa zea

-

the enzyme plays an essential role in intracellular membrane transport events

667820

-

-

?

ATP + H2O

ADP + phosphate

Homo sapiens

-

-

210576, 210577, 210578, 210580, 210581, 210583, 210585, 656661, 669490, 756027, 756698, 758466

-

-

?

ATP + H2O

ADP + phosphate

Homo sapiens

P55072

-

756637

-

-

?

ATP + H2O

ADP + phosphate

Homo sapiens

-

Pex1p possesses two distinct oligomeric forms, a homo-oligomer in the cytosol and a hetero-oligomer on peroxisome membranes, possibly playing distinct functions in peroxisome biogenesis

669490

-

-

?

ATP + H2O

ADP + phosphate

Komagataella pastoris

-

-

210580, 210586

-

-

?

ATP + H2O

ADP + phosphate

Mammalia

-

-

686724, 688499

-

-

ir

ATP + H2O

ADP + phosphate

Mus musculus

-

-

210576, 210577, 210578, 210579, 210585, 656346, 656763, 755920

-

-

?

ATP + H2O

ADP + phosphate

Mus musculus

-

-

687536, 687629

-

-

ir

ATP + H2O

ADP + phosphate

Mus musculus

-

ATPase N-ethylmaleimide sensitive factor may be a common feature in the triggering/regulation of membrane merging during mammalian acrosome reaction

667294

-

-

?

ATP + H2O

ADP + phosphate

Mus musculus

-

N-ethylmaleimide-sensitive factor is required for the synaptic incorporation and removal of 2-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors during cerebellar long-term depression

670662

-

-

?

ATP + H2O

ADP + phosphate

Mus musculus

-

NSF is a critical regulator of leukocyte trafficking in vivo

669996

-

-

?

ATP + H2O

ADP + phosphate

Mus musculus

-

PEX1 is indispensable for biogenesis and maintenance of the peroxisome

668578

-

-

?

ATP + H2O

ADP + phosphate

protozoa

-

-

688690

-

-

ir

ATP + H2O

ADP + phosphate

Rattus norvegicus

-

-

210581, 210584, 210585, 656341, 656874

-

-

?

ATP + H2O

ADP + phosphate

Rattus norvegicus

-

-

686380, 686701, 686724, 687536

-

-

ir

ATP + H2O

ADP + phosphate

Rattus norvegicus

-

coordinated action of NSF and PKC regulates GABAB receptor signaling efficacy

668410

-

-

?

ATP + H2O

ADP + phosphate

Rattus norvegicus

-

the enzyme plays a crucial role in the fusion of vesicles with target membranes. NSF-SNAP complexes may be involved in the disassembly of coiled-coil-mediated interactions underlying not only SNARE complexes but also other forms of stable oligomers, and the C-terminal membrane anchoring of NSF-SNAP targets may be mechanistically involved in the dissociation process

669601

-

-

?

ATP + H2O

ADP + phosphate

Saccharomyces cerevisiae

-

-

210576, 210577, 210578, 210579, 210582, 210584, 210585, 654648, 656345, 668565, 757152

-

-

?

ATP + H2O

ADP + phosphate

Saccharomyces cerevisiae

-

-

668565, 686724, 688690

-

-

ir

ATP + H2O

ADP + phosphate

Saccharomyces cerevisiae

P52917

-

734303

-

-

?

ATP + H2O

ADP + phosphate

Saccharomyces cerevisiae

-

Vps4 disassembles the ESCRT-III protein polymer, thereby changing the morphology of the underlying membrane. The disassembly reaction causes the ESCRT-III subunits to regain the monomeric conformational state, which is the high-energy state that is poised to reassemble again into the ESCRT-III oligomer for subsequent rounds of membrane scission. When assembled, Vps4 progresses through many ATP hydrolysis cycles without dissociation

724447

-

-

?

ATP + H2O

ADP + phosphate

Schizosaccharomyces pombe

-

-

210577, 210578

-

-

?

ATP + H2O

ADP + phosphate

Sulfolobus acidocaldarius

-

-

210576

-

-

?

ATP + H2O

ADP + phosphate

Xenopus laevis

-

-

210576, 210584

-

-

?

additional information

?

-

Cricetulus griseus

-

modeling of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. Subsequent additional alpha-SNAP binding events may occur as part of a processive disassembly mechanism

734226

-

-

?

additional information

?

-

Dictyostelium discoideum

Q75JI3

UBX-domain containing cytoplasmic Dictyostelium protein UBXD9 is identified as p97 interaction partner

756637

-

-

-

additional information

?

-

Saccharomyces cerevisiae

P52917

residues from helix 5 of the Vps2p subunit of ESCRT-III bind to the central pore of an asymmetric Vps4p hexamer in a manner that is dependent upon the presence of flexible nucleotide analogues that can mimic multiple states in the ATP hydrolysis cycle, quantitative binding studies. Vps2p helix 5 peptides bind the Vps4p ATPase cassette. Vps4 substrates are initially recruited by an MIM-MIT interaction that activates the Vps4 central pore to engage substrates and generate force, thereby triggering ESCRT-III disassembly. The Vps4p hexamer binds a single peptide

734303

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins

735147

-

-

?

additional information

?

-

Saccharomyces cerevisiae BY4741

-

the tombusvirus p33 replication protein interacts with Vps4p and three ESCRT-III proteins

735147

-

-

?