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3.6.4.6: vesicle-fusing ATPase

This is an abbreviated version!
For detailed information about vesicle-fusing ATPase, go to the full flat file.

Word Map on EC 3.6.4.6

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

AAA ATPase Vps4, AAA-ATPase, ATP phosphohydrolase, ATP6V0D1, ATP6V0D2, ATPase, ATPase N-ethylmaleimide sensitive factor, C06A1.1, C41C4.8, CDC-48.1, CDC-48.2, Cdc48, Cdc48p, Csc1p, Did6p, dNSF, dNSF-1, dNSF1, End13p, Grd13p, Helar-NSF, Hez-NSF, HsPEX1, HsPex1p, HsPex6p, membrane deformation AAA ATPase, N-ethyl maleimide sensitive factor, N-ethylmaleimide sensitive factor, N-ethylmaleimide sensitive fusion protein, N-ethylmaleimide sensitive-factor, N-ethylmaleimide-sensitive factor, N-ethylmaleimide-sensitive fusion protein, N-ethylmeleimide-sensitive factor, NEM-sensitive fusion protein, NSF, NSF ATPase, NSF protein, NSF-1, NSF2, nsfA, NSFII, p97, p97/VCP/Cdc48p, p97/VCP/Cdc48p homologue, p97ATPase, peptide p97, Pex1, Pex1p, pex22, Pex6p, PpPex1p, PpPex6p, rat Pex6p, Sec18, SEC18 gene product, SEC18p, SKD1, SKD1/VPS4B, SKD2, SKD2 protein, Sso0909, SsoVps4, suppresor of potassium transport growth defect, Suppressor of K+ Transport Growth Defect1, triple-A ATPase, V-ATPase, vacular protein sorting 4 B, vacuolar protein sorting 4, vacuolar protein sorting 4 protein, vasolin-containing protein, VCP, vesicle-fusing ATPase, Vesicular-fusion protein NSF, Vpl4p, Vps 4B, Vps4, Vps4 ATPase, VPS4 gene product, Vps4p, Vps4p AAA ATPase, Vpt10p

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.6 vesicle-fusing ATPase

Cloned

Cloned on EC 3.6.4.6 - vesicle-fusing ATPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA containing the NSF D1 ATPase domain is inserted into the pET28a vector for expression in Escherichia coli BL21 cells
-
cloning of PEX2, PEX6, PEX12 and PEX1 using yeast genes for homology search
-
constructing of a genomic library from the haploid yeast strain SD228-7 using the Escherichia coli-yeast shuttle vector pCS19
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysE cells
expressed in Escherichia coli Rosetta 2 (DE3) cells
expression in Escherichia coli
expression in rat 3Y1B fibroblast cells
-
expression in U2OS TRex cells
-
full-length, partial cDNA fragments and point mutations of mouse NSF are amplified by PCR and subcloned into different expression vectors including pGEX-6P-2, pETH-32, pcDNA3-His6, and pcDNA3-3HA
-
His6-NSF for expression in Escherichia coli BL21 cells
-
isolation of PEX2 and PEX6 cDNA, Pex1p expressed in wild-type chinese hamster ovary cells, CHO-K1
-
N-terminal domain of PEX1
-
recombinant expression of GST- and His6-tagged Vps4p and Vps4101–437 in Saccharomyces cerevisiae strain BY4741
-
recombinant expression of GST-tagged wild-type and mutant p97 in Escherichia coli strain Arctic express RIL, coexpression with GST-tagged human UBXD9 protein in Escherichia coli in strain BL21Gold (DE3) pLysS
recombinant expression of GST-tagged wild-type and mutant p97 in Escherichia coli strain XL1 Blue, coexpression with GST-tagged Dictyostelium UBXD9 protein
recombinant expression of His6-tagged enzyme NSF in Escherichia coli strain BL21(DE3), coexpression of alpha-SNAP, VAMP2(1-96), syntaxin1A(1-265)-C145S/S249C/K253C, syntaxin1A(191-265)-C145S/S249C/K253C, and SNAP25A
-
recombinant expression of wild-type and mutant Vps4ps
SKD1 and GFP5 PCR-amplified fragments are cloned in pBSII KS+ and subsequently subcloned into pMZ215S, and in the binary vectors pCambia 1300 and pbinSRNACatN
-
SKD1 cDNA amplified by reverse transcription-PCR
-
the vectors YCplac111, pLexA, pET11d and pB42AD are used for cloning and expression of wild-type protein, single domains and various mutants
-
VPS24 gene cloned from a yeast genomic library
-
VPS4 gene cloned and overexpressed in Escherichia coli XL1-blue, using a GST fusion vector
-