3.6.1.7: acylphosphatase This is an abbreviated version!go to the full flat file .
Reaction
an acylphosphate H2O a carboxylate phosphate
Synonyms 1,3-diphosphoglycerate phosphatase, acetic phosphatase, acetyl phosphatase, acetylphosphatase, ACP, AcPDRo2, acyl phosphatase, acyl phosphate phosphohydrolase, Acylphosphatase, erythrocyte isozyme, Acylphosphatase, erythrocyte/testis isozyme, Acylphosphate phosphohydrolase, acylphosphate phosphomonohydrolase, Acyp, ACYP2, carbamoylphosphate phosphatase, carbamyl phosphate phosphatase, Ch1, Ch2, GP 1-3, GP1, GP2, GP3, Ho 1-3, Ho1, Ho2, Ho3, human common-type acylphosphatase, Isozyme CH1, Isozyme CH2, Isozyme TU1, More, muscle-type acylphosphatase 2, native acylphosphatase, PhAcP, phosphatase, acyl, Sso AcP, SSO0887, T1, TT0497
ECTree
Temperature Stability
Temperature Stability on EC 3.6.1.7 - acylphosphatase
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112
melting temperature: 111.5°C
25 - 100
At 25°C, the free energy of unfolding, midpoint of transition, and m value were 54.4 kJ/mol, 4.99 M, and 10.9 kJ/mol/M, respectively. The melting temperature is about 111.5°C. These data indicate that PhAcP is an extremely thermostable protein.
65
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muscular isoenzyme, fully denatured protein. Thermal denaturation is reversibel
70
-
erythrocyte enzyme, 5 min: 40% loss of activity
80
-
brain enzyme, partially purified, 15 min, pH 1: 100% activity retained, pH 7: 80-90% activity retained
85
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heart enzyme, pH 2.0, 5 min, 95% activity retained
90
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no disruption of three-dimensional structure when incubated at 90°C for 30min
100
-
2 h: heat inactivation
100
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2 h: heat inactivation
100
-
melting temperature
37
-
brain enzyme, purified and dialyzed enzyme, 20 min, 27% inactivation, undialyzed enzyme: no inactivation
37
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90% loss of activity after 10 min at, pH range 5.3-7.4
additional information
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enzyme is thermostable
additional information
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enzyme is thermostable
additional information
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enzyme is thermostable
additional information
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enzyme is thermostable
additional information
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additional information
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muscle enzyme, unusually thermostable
additional information
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no cold denaturation
additional information
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thermal inactivation studies
additional information
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increase of urea concentration causes reduction of the thermoresistance of the protein
additional information
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muscle and brain enzymes: stable at high temperatures
additional information
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studies on conformational stability of muscle acylphosphatase
additional information
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enzyme is thermostable
additional information
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enzyme is thermostable, thermostability is pH-dependent, turkey enzyme is less unstable at higher pH values than horse enzyme
3.6.1.7 acylphosphatase
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