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3.6.1.7: acylphosphatase

This is an abbreviated version!
For detailed information about acylphosphatase, go to the full flat file.

Word Map on EC 3.6.1.7

Reaction

an acylphosphate
+
H2O
=
a carboxylate
+
phosphate

Synonyms

1,3-diphosphoglycerate phosphatase, acetic phosphatase, acetyl phosphatase, acetylphosphatase, ACP, AcPDRo2, acyl phosphatase, acyl phosphate phosphohydrolase, Acylphosphatase, erythrocyte isozyme, Acylphosphatase, erythrocyte/testis isozyme, Acylphosphate phosphohydrolase, acylphosphate phosphomonohydrolase, Acyp, ACYP2, carbamoylphosphate phosphatase, carbamyl phosphate phosphatase, Ch1, Ch2, GP 1-3, GP1, GP2, GP3, Ho 1-3, Ho1, Ho2, Ho3, human common-type acylphosphatase, Isozyme CH1, Isozyme CH2, Isozyme TU1, More, muscle-type acylphosphatase 2, native acylphosphatase, PhAcP, phosphatase, acyl, Sso AcP, SSO0887, T1, TT0497

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.7 acylphosphatase

Purification

Purification on EC 3.6.1.7 - acylphosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation and cation exchange chromatography
-
C21S mutant
-
erythrocyte form isoenzyme
-
erythrocyte isoenzyme
-
from breast and leg muscles
-
glutathione column chromatography
-
heart muscle
heart, cardiac muscle
-
HiTrap SP HP column chromatography and Superdex 75 column chromatography
multiple molecular forms: Ho1, Ho2 and Ho3
-
muscle and liver
-
muscle, erythrocyte isoenzyme
-
muscle, erythrocyte type enzyme
-
muscular isoenzyme as fusion protein with glutathione S-transferase, subsequently cleaved to fully active acylphosphatase and further purified
-
partially from breast muscle
-
recombinant enzyme from Escherichia coli by cation exchange chromatography and gel filtration
-
recombinant muscle type and common type isoenzymes
-
recombinant TT0497 from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography, and gel filtration
recombinant wild-type and Cys21 mutants: C21A and C21S
-
recombinant wild-type and mutated acylphosphatases
-
testis and skeletal muscle
-
two isozymes: Ch1 and Ch2
-