mutant enzyme lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days. The mutant enzyme is less susceptible to oxidative inactivation than the wild-type enzyme
when clade I Arabidopsis apyrases are expressed in the DELTAynd1DELTAgda1 dKO Saccharomyces cerevisiae mutant, both AtAPY1 and AtAPY2 are able to complement the growth phenotype compared to the yeast mutant harbouring the empty vector
when clade I Arabidopsis apyrases are expressed in the DELTAynd1DELTAgda1 dKO Saccharomyces cerevisiae mutant, both AtAPY1 and AtAPY2 are able to complement the growth phenotype compared to the yeast mutant harbouring the empty vector
introduction of the wild-type SA1684 gene restores the hemolysin production of the SA1684-deletion mutant, whereas none of the alanine-substituted SA1684 mutant genes restores the hemolysin production. Construction of a DELTASA1684 deletion mutant, which exhibits drastically decreased virulence in a silkworm (Bombyx mori) infection model, in which the LD50 against silkworm larvae is more than 10times that of the parent strain. The SA1684-deletion mutant also exhibits decreased exotoxin production and colony-spreading ability. Introduction of wild-type SA1684 to the SA1684-deletion mutant restores the hemolysin production, nuclease production, and the colony-spreading activity
introduction of the wild-type SA1684 gene restores the hemolysin production of the SA1684-deletion mutant, whereas none of the alanine-substituted SA1684 mutant genes restores the hemolysin production. Construction of a DELTASA1684 deletion mutant, which exhibits drastically decreased virulence in a silkworm (Bombyx mori) infection model, in which the LD50 against silkworm larvae is more than 10times that of the parent strain. The SA1684-deletion mutant also exhibits decreased exotoxin production and colony-spreading ability. Introduction of wild-type SA1684 to the SA1684-deletion mutant restores the hemolysin production, nuclease production, and the colony-spreading activity
introduction of the wild-type SA1684 gene restores the hemolysin production of the SA1684-deletion mutant, whereas none of the alanine-substituted SA1684 mutant genes restores the hemolysin production. Construction of a DELTASA1684 deletion mutant, which exhibits drastically decreased virulence in a silkworm (Bombyx mori) infection model, in which the LD50 against silkworm larvae is more than 10times that of the parent strain. The SA1684-deletion mutant also exhibits decreased exotoxin production and colony-spreading ability. Introduction of wild-type SA1684 to the SA1684-deletion mutant restores the hemolysin production, nuclease production, and the colony-spreading activity