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3.6.1.28: thiamine-triphosphatase

This is an abbreviated version!
For detailed information about thiamine-triphosphatase, go to the full flat file.

Word Map on EC 3.6.1.28

Reaction

thiamine triphosphate
+
H2O
=
thiamine diphosphate
+
phosphate

Synonyms

25 kDa thiamine triphosphatase, 25-kDa thiamine triphosphatase, phosphatase, thiamine tri-, thiamine triphosphatase, Thtpa, ThTPase, TTPase

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.28 thiamine-triphosphatase

Specific Activity

Specific Activity on EC 3.6.1.28 - thiamine-triphosphatase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0003
quail
-
activity in quadriceps
0.0006
0.0008
quail
-
activity in kidney
0.0024
quail
-
activity in liver
0.0114
-
activity in brain
0.0125
-
activity in skeletal muscle
0.0162
-
activity in kidney
0.033
-
duodenum
0.041
-
purified GST-tagged thiamine triphosphatase, 37°C, pH 8.2, 4 mM MgCl2, 0.5 mM thiamine triphosphate
0.048
-
wild type enzyme from supernatant, at 37°C, 50 mM Na-TAPS pH 8.5, 8 mM MgCl2, 0.5 mM thiamine triphosphate
0.052
-
activity in quadriceps
0.057
-
cerebellum
0.09
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 5 mM Na-EDTA
0.169
-
trapezius muscle
0.189
-
activity in kidney
0.217
-
heart
0.22
-
lymph node
0.275
-
spleen
0.299
-
activity in liver
0.309
-
brain
0.333
-
lung
0.354
-
liver
0.377
-
kidney
0.437
-
activity in brain
0.45
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 0.04 mM ZnSO4
0.55
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 0.2 mM ZnSO4
0.59
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 4 mM MgCl2 + 4 mM CaCl2
0.63
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 0.1 mM ZnSO4
0.932
-
activity in pectoralis muscle
0.99
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 4 mM MgCl2 + 1 mM CaCl2
1.7
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 4 mM MgCl2
1.9
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 4 mM MgCl2 + 0.1 mM ZnSO4
140
-
purified recombinant enzyme
2.55
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 4 mM MgCl2 + 0.04 mM ZnSO4
225
pure untagged recombinant thiamine triphosphatase, 37°C, pH 8.2
3.08
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 1 mM MnCl2
3.3
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 0.05 mM MnCl2
3.45
-
K85E-mutated GST-tagged thiamine triphosphatase, pH 8.2, in the presence of 0.1 mM MnCl2
4.5
wild type thiamine triphosphatase from supernatant
648
-
-