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3.6.1.15: nucleoside-triphosphate phosphatase

This is an abbreviated version!
For detailed information about nucleoside-triphosphate phosphatase, go to the full flat file.

Word Map on EC 3.6.1.15

Reaction

a nucleoside triphosphate
+
H2O
=
a nucleoside diphosphate
 +
phosphate

Synonyms

AAA-type nucleoside triphosphatase phosphohydrolase, ankyrin repeat-rich membrane spanning protein, cell division control protein Cdc48, DEAH-box Prp22 protein, DEAH-box splicing factor Prp22, DExH-Box NTPase, DR_0079, germ-cell-specific leucine-rich repeat protein, GP086L protein, HCR-NTPase, HrpB, HrpB NTPase, human cancer-related nucleoside triphosphatase, KAP NTPase, Kidins220, kinase D interacting substance of 220 kDa, LDBPK_100150, LdNTPDase2, LINF_150005200, LiNTPDase1, LMXM_10_0170, LMXM_15_0030, LmxNTPDase1, LmxNTPDase2, M86L protein, MC100R, Mg2+-dependent NTPase, More, Msm0858, NALP14, non-specific nucleoside triphosphatase, nonstructural protein 13, NPH I, NS3, NS3 protein, NS3FL, nsp13, NTP1, NTP3, NTPase, NTPase 1, NTPase 2, NTPase-I, NTPase-II, NTPase/helicase, NTPDase, NTPDase 1, NTPDase1, NTPDase2, nucleic acid-independent nucleoside triphosphatase, nucleoside 5-triphosphatase, nucleoside triphosphatase, nucleoside triphosphatase (NTPase)/helicase, nucleoside triphosphate diphospho-hydrolase, nucleoside triphosphate diphosphohydrolase, nucleoside triphosphate diphosphohydrolase 1, nucleoside triphosphate hydrolase, nucleoside triphosphate hydrolases I, nucleoside triphosphate hydrolases II, nucleoside triphosphate phosphohydrolase, nucleoside triphosphate phosphohydrolase I, nucleoside-5-triphosphate phosphohydrolase, nucleoside-triphosphatase 1, nucleoside-triphosphatase 2, nucleotide 5'-triphosphatase, P-loop NTPase, phosphatase, nucleoside tri-, PilB, PilT, PilU, polypeptide p41, protein NS3, PVA coat protein, PVA CP, PVx coat protein, PVX CP, Rad55B, RNA-dependent nucleoside triphosphatase, SHVp41, Sso0909, T4P motor protein, type IV pilus motor protein, YtkD

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.15 nucleoside-triphosphate phosphatase

Purification

Purification on EC 3.6.1.15 - nucleoside-triphosphate phosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration, SDS-PAGE
-
native enzyme from virus Vero E6 cell culture medium by affinity chromatography, ultrafiltration, and gel filtration
-
partially, isolation of hepatic nuclei
-
phosphohydrolase II
-
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli strain HMS174 by streptomycin sulfate treatment and gel filtration
-
recombinant GST-tagged Sso0909 from Escherichia coli by glutathione affinity chromatography and gel filtration, recombinant His10-tagged Sso0909 from Sulfolobus solfataricus by nickel affinity chromatography and gel filtration to homogeneity
recombinant His-tagged DEAH-box Prp22 protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
-
recombinant His-tagged enzyme from insect cells
-
recombinant His-tagged full-length viral NS3 protein and N-terminal truncated version ntNS3 from Escherichia coli by nickel affinity chromatography and dialysis
-
recombinant His6-tagged coat protein from Escherichia coli by nickel affinity chromatogryphy
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatography
-
recombinant MBP-tagged enzyme from Escherichia coli by amylose affinity chromatography and gel filtration. When the MBP-NS3 is treated with TEV protease to remove its MBP tag, the free NS3 becomes unstable and aggregates
-
recombinant NS3 from Escherichia coli
-
recombinant soluble His-tagged enzyme from Escherichia coli strain Rosetta Gammi (DE3) to homogeneity by nickel affinity chromatography and dialysis
recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange chromatography and gel filtration to homogeneity
-
recombinant wild-type or SeMet-labeled His10-tagged enzyme from Escherichia coli strains BL21(DE3) or B834 by nickel affinity chromatography, tag cleavage by Smt3-specific protease Ulp1, a second step of nickel affinity chromatography, and ultrafiltration, followed by dialysis
recombinat His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
Toyopearl DEAE-650S column chromatography, Source 15Q column chromatography, and Superdex 200 pg gel filtration
transcriptase cofactor my2 from host nucleoplasma by ion exchange and adsorption chromatography at room temperature
-