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157 - 458
(phosphate)13-18
-
0.16
(phosphate)25
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
-
0.22
(phosphate)45
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
-
0.03
(phosphate)65
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
-
1550
(Phosphate)n
-
37°C, recombinant enzyme
6.7
(polyphosphate)130
pH 6.8, 37°C
-
10.2
(polyphosphate)14
pH 6.8, 37°C
-
7.3
(polyphosphate)60
pH 6.8, 37°C
-
0.05
(Polyphosphate)n
+/- 0.005
31 - 723
adenosine 5'-tetraphosphate
40
adenosine-5'-pentaphosphate
-
-
12.555
ADP
pH 7.4, 37°C, recombinant enzyme
22.649
ATP
pH 7.4, 37°C, recombinant enzyme
0.7 - 308
guanosine 5'-tetraphosphate
3.55
p-nitrophenyl phosphate
+/- 0.16
8.1
pentasodium triphosphate
in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C
0.14 - 1150
Polyphosphate
7.26 - 40.2
polyphosphate25
5.29 - 41.23
polyphosphate45
-
27.95 - 44.11
polyphosphate60
-
6.86 - 53.03
polyphosphate65
-
2.512 - 16.39
polyphosphate700
-
6.69 - 57.02
polyphosphate75
-
0.9
Tetraphosphate
in 50 mM PIPES, pH 6.8 containing 25 mM KCl and 2 mM MgCl2
143 - 180
tripolyphosphate
additional information
additional information
-
-
-
157
(phosphate)13-18
pH 6.5, 25°C
-
458
(phosphate)13-18
pH 6.5, 25°C
-
0.57
(phosphate)3
-
H107N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
4.2
(phosphate)3
-
H108N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
4.6
(phosphate)3
-
D106A mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
13
(phosphate)3
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
13.5
(phosphate)3
pH 6.5, 25°C
19
(phosphate)3
-
R128H mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
35
(phosphate)3
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
399
(phosphate)3
pH 6.5, 25°C
3.4
(phosphate)4
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
7.1
(phosphate)4
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
31
adenosine 5'-tetraphosphate
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
39
adenosine 5'-tetraphosphate
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
723
adenosine 5'-tetraphosphate
-
-
0.7
guanosine 5'-tetraphosphate
pH 7.5, 30°C
14.2
guanosine 5'-tetraphosphate
pH 6.5, 25°C
24
guanosine 5'-tetraphosphate
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
27
guanosine 5'-tetraphosphate
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
308
guanosine 5'-tetraphosphate
pH 6.5, 25°C
0.14
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant D127N
0.52
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant H149N
0.66
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant H148N
2.2
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant N35H
1150
Polyphosphate
-
pH 7.2, 25°C, recombinant wild-type enzyme
7.26
polyphosphate25
-
pH 8.0, 37°C, truncated enzyme
40.2
polyphosphate25
-
pH 8.0, 37°C, full-length enzyme
5.29
polyphosphate45
-
pH 8.0, 37°C, truncated enzyme
-
41.23
polyphosphate45
-
pH 8.0, 37°C, full-length enzyme
-
27.95
polyphosphate60
pH 7.4, 37°C, recombinant enzyme
-
44.11
polyphosphate60
pH 7.4, 37°C, recombinant enzyme
-
6.86
polyphosphate65
-
pH 8.0, 37°C, truncated enzyme
-
53.03
polyphosphate65
-
pH 8.0, 37°C, full-length enzyme
-
2.512
polyphosphate700
pH 7.4, 37°C, recombinant enzyme
-
16.39
polyphosphate700
pH 7.4, 37°C, recombinant enzyme
-
6.69
polyphosphate75
-
pH 8.0, 37°C, truncated enzyme
-
57.02
polyphosphate75
-
pH 8.0, 37°C, full-length enzyme
-
0.6
Triphosphate
in 50 mM PIPES, pH 6.8 containing 25 mM KCl and 2 mM MgCl2
3.46
Triphosphate
pH 7.5, 30°C
143
tripolyphosphate
-
-