3.6.1.11: exopolyphosphatase This is an abbreviated version! For detailed information about exopolyphosphatase, go to the full flat file .
Reaction
(Polyphosphate)n +
H2O =
(polyphosphate)n-1 +
phosphate
Synonyms 40 kD exopolyphosphatase, 40-kDa-exopolyphosphatase, acid phosphoanhydride phosphohydrolase, CJJ81176_0377, CJJ81176_1251, CT0099, CT1713, ecPpx, exopoly(P)ase, ExopolyPase, exopolyphosphatase, exopolyphosphatase 1, exopolyphosphatase 2, Gra-Pase, h-prune, high molecular mass exopolyphosphatase, high molecular weight exopolyphosphatase, high-molecular exopolyphosphatase, LmPPX, major cytosolic exopolyphosphatase PPX1, membrane-bound exopolyphosphatase, metaphosphatase, More, Msed_0981, MT0516, NMB1467, nuclear exopolyphosphatase, Nudix hydrolase, paPpx, phosphatase, exopoly-, polyphosphate phosphatase, polyphosphate phosphohydrolase, polyphosphate-phosphohydrolase, Ppn1, PPX, Ppx protein, Ppx/GppA phosphatase, PPX1, PPX2, PPXI, PPXMsed, Rv0496, Tb927.5.4350, Tb927.6.2670, TbDcp2, TbNH2, TbNH4, vacuolar exopolyphosphatase, YHR201C, Za10_0559, ZmPPX
ECTree
Temperature Stability
Temperature Stability on EC 3.6.1.11 - exopolyphosphatase
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0
-
incubation of h-prune 1-100 microM for 5 h in the presence of 0.1 M Tris-HCl, pH 7.2, and 0.05 mM EGTA inactivates the enzyme 2-4fold, no inactivation is evident in the presence of 1 mM Mg2+
25 - 40
at pH 6.8, 2 mM MgCl2 and 25 mM KCl, no significant loss of activity is observed after 60 min
40
-
15 min, 70% loss of activity
45
after preheating at 45°C for 30 and 60 min, 70% and 40% of the PPX2 activity remains
50
after preheating at 50°C, His-tagged PPX2 loses its activity quickly, after incubation for 60 min, no activity remains
70
-
10 min, inactivation
60
complete inactivation of His-tagged PPX2 within 5 min
60
-
2 min, complete loss of activity, slight protection from heat inactivation, 10%, by 1 mM MnCl2