3.6.1.11: exopolyphosphatase
This is an abbreviated version!
For detailed information about exopolyphosphatase, go to the full flat file.
Word Map on EC 3.6.1.11
Reaction
Synonyms
40 kD exopolyphosphatase, 40-kDa-exopolyphosphatase, acid phosphoanhydride phosphohydrolase, CJJ81176_0377, CJJ81176_1251, CT0099, CT1713, ecPpx, exopoly(P)ase, ExopolyPase, exopolyphosphatase, exopolyphosphatase 1, exopolyphosphatase 2, Gra-Pase, h-prune, high molecular mass exopolyphosphatase, high molecular weight exopolyphosphatase, high-molecular exopolyphosphatase, LmPPX, major cytosolic exopolyphosphatase PPX1, membrane-bound exopolyphosphatase, metaphosphatase, More, Msed_0981, MT0516, NMB1467, nuclear exopolyphosphatase, Nudix hydrolase, paPpx, phosphatase, exopoly-, polyphosphate phosphatase, polyphosphate phosphohydrolase, polyphosphate-phosphohydrolase, Ppn1, PPX, Ppx protein, Ppx/GppA phosphatase, PPX1, PPX2, PPXI, PPXMsed, Rv0496, Tb927.5.4350, Tb927.6.2670, TbDcp2, TbNH2, TbNH4, vacuolar exopolyphosphatase, YHR201C, Za10_0559, ZmPPX
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3.6.1.11 exopolyphosphataseAdvanced search results
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Substrates Products on EC 3.6.1.11 - exopolyphosphatase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(phosphate)9 + H2O
(phosphate)8 + phosphate
45% of the activity with (phosphate)25
-
-
?
polyphosphate + H2O
?
-
chain length of more than 45 phosphate residues
-
-
?
tetraphosphate + H2O
triphosphate + phosphate
-
-
-
?
(phosphate)13-18 + H2O
(phosphate)12-17 + phosphate
-
-
-
?
(phosphate)13-18 + H2O
(phosphate)12-17 + phosphate
-
-
-
?
(phosphate)15 + H2O
(phosphate)14 + phosphate
88% of the activity with (phosphate)25
-
-
?
(phosphate)208 + H2O
(phosphate)207 + phosphate
-
reaction continues to a chain length of about 15 residues
-
?
(phosphate)208 + H2O
(phosphate)207 + phosphate
100% activity
-
-
?
(phosphate)25 + H2O
(phosphate)24 + phosphate
100% activity
-
-
?
(phosphate)3 + H2O
(phosphate)2 + phosphate
best substrate
-
-
?
(phosphate)3 + H2O
(phosphate)2 + phosphate
-
-
-
?
(phosphate)3 + H2O
(phosphate)2 + phosphate
best substrate
-
-
?
(phosphate)3 + H2O
(phosphate)2 + phosphate
14% of the activity with (phosphate)25
-
-
?
(phosphate)300 + H2O
(phosphate)299 + phosphate
or even longer chain length
-
-
?
(phosphate)300 + H2O
(phosphate)299 + phosphate
or even longer chain length
-
-
?
(phosphate)45 + H2O
(phosphate)44 + phosphate
100% activity
-
-
?
(phosphate)n + H2O
(phosphate)n-1 + phosphate
degradation of polyphosphate
-
-
?
(phosphate)n + H2O
(phosphate)n-1 + phosphate
-
h-prune efficiently hydrolyzes short-chain polyphosphates
-
-
?
(phosphate)n + H2O
(phosphate)n-1 + phosphate
-
-
-
?
(phosphate)n + H2O
(phosphate)n-1 + phosphate
-
regulatory enzyme in polyphosphate metabolism
-
-
?
(phosphate)n + H2O
(phosphate)n-1 + phosphate
-
-
-
-
?
(phosphate)n + H2O
(phosphate)n-1 + phosphate
-
the cytosolic exopolyphosphatase that processively cleaves the terminal phosphate group from the polyphosphate chain, until inorganic diphosphate is all that remains, structure of the substrate binding channel, overview
-
-
?
(polyphosphate)130 + H2O
(polyphosphate)129 + phosphate
-
-
-
?
(polyphosphate)130 + H2O
(polyphosphate)129 + phosphate
-
-
-
?
(polyphosphate)14 + H2O
(polyphosphate)13 + phosphate
-
-
-
?
(polyphosphate)14 + H2O
(polyphosphate)13 + phosphate
-
-
-
?
(polyphosphate)60 + H2O
(polyphosphate)59 + phosphate
-
-
-
?
(polyphosphate)60 + H2O
(polyphosphate)59 + phosphate
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
phosphate starvation induces the formation of the enzyme
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
enzyme is derepressed under phosphate starvation conditions
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
no acyivity if n = 200
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
n = 40, 72, 180 or 290, highest reaction rate, when n is 40
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
polyphosphatase I shows optimal activity when n is 25 phosphate residues, polyphosphatases II prefers substrates with 9 residues
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
n = 500 - 600
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
the activity with polyP15 is 85% of that with polyP208, the activity with polyP9 is 24% of that with polyP208
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
poly P9-10, polyP33-36
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
n = 10, 25, 50, 100, 250 or 500. n = 250 is the preferred substrate
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
polyP15, polyP208
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
chain length of 10-200 phosphate residues
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
chain length of 10-200 phosphate residues
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
-
-
-
?
(polyphosphate)n + H2O
(polyphosphate)n-1 + phosphate
-
the inhibitory effect of long-chain polyphosphates on adenylate kinase is higher than that of short-chain polyphosphates, suggesting a potential role of polyphosphate metabolism in regulating intracellular concentration of adenylate nucleotides
-
-
?
adenosine 5'-tetraphosphate + H2O
ATP + phosphate
-
7.3% of the activity with polyP208
-
-
?
ATP + 2 H2O
AMP + 2 phosphate
-
-
-
?
diphosphate + H2O
2 phosphate
only in presence of Co2+, not with Mg2+, reaction of EC 3.6.1.1
-
-
?
diphosphate + H2O
2 phosphate
only in presence of Co2+, not with Mg2+, reaction of EC 3.6.1.1
-
-
?
guanosine 5'-tetraphosphate + H2O
GTP + phosphate
-
-
-
?
guanosine 5'-tetraphosphate + H2O
GTP + phosphate
-
-
-
?
guanosine tetraphosphate + H2O
guanosine triphosphate + phosphate
-
-
-
?
guanosine tetraphosphate + H2O
guanosine triphosphate + phosphate
-
-
-
?
pentasodium triphosphate + H2O
pentasodium diphosphate + phosphate
-
-
-
?
pentasodium triphosphate + H2O
pentasodium diphosphate + phosphate
-
-
-
?
polyphosphate 15 + H2O
polyphosphate 14 + phosphate
-
-
-
-
?
polyphosphate 208 + H2O
polyphosphate 207 + phosphate
-
-
-
?
polyphosphate 208 + H2O
polyphosphate 207 + phosphate
-
-
-
-
?
polyphosphate 208 + H2O
polyphosphate 207 + phosphate
-
-
-
-
?
polyphosphate 3 + H2O
polyphosphate 2 + phosphate
-
-
-
-
?
polyphosphate130 + H2O
polyphosphate129 + phosphate
-
-
-
?
polyphosphate60 + H2O
polyphosphate59 + phosphate
-
-
-
?
polyphosphate60 + H2O
polyphosphate59 + phosphate
-
-
-
?
polyphosphate700 + H2O
polyphosphate699 + phosphate
-
-
-
?
polyphosphate700 + H2O
polyphosphate699 + phosphate
-
-
-
?
tripolyphosphate + H2O
phosphate + ?
-
7.3% of the activity with polyP208
-
-
?
additional information
?
-
isoform Ppx1 shows substantial nucleoside triphosphatase activity
-
-
?
additional information
?
-
isoform Ppx1 shows substantial nucleoside triphosphatase activity
-
-
?
additional information
?
-
isoform Ppx1 shows substantial nucleoside triphosphatase activity
-
-
?
additional information
?
-
isoform Ppx1 shows substantial nucleoside triphosphatase activity
-
-
?
additional information
?
-
PPX2 is active with short-chain polyphosphates, even accepting diphosphate
-
-
?
additional information
?
-
PPX2 is active with short-chain polyphosphates, even accepting diphosphate
-
-
?
additional information
?
-
-
PPX2 is active with short-chain polyphosphates, even accepting diphosphate
-
-
?
additional information
?
-
-
contains an exopolyphosphatase, EC 3.6.1.11 and a guanosine pentaphosphate phosphohydrolase with long-chain exopolyphosphatase activity, EC 3.6.1.40
-
-
?
additional information
?
-
exopolyphosphatase Ppx releases inorganic phosphate (Pi) from polyphosphate. Inorganic polyphosphate (polyP) is a linear anionic polymer of phosphate molecules which was found in all living organisms and may form aggregates. The phosphate molecules within polyphosphate are held together by high-energy phosphoanhydride bonds. The length of this polymer may vary between ten to hundreds of units, depending on the organism and its physiological stage
-
-
-
additional information
?
-
-
exopolyphosphatase Ppx releases inorganic phosphate (Pi) from polyphosphate. Inorganic polyphosphate (polyP) is a linear anionic polymer of phosphate molecules which was found in all living organisms and may form aggregates. The phosphate molecules within polyphosphate are held together by high-energy phosphoanhydride bonds. The length of this polymer may vary between ten to hundreds of units, depending on the organism and its physiological stage
-
-
-
additional information
?
-
substrates are commercial sodium phosphate glass with 25-PP25-, 65-PP65-, or PPK-synthesized polyphosphate with 700-PP700-average number of residues. Released phosphate is detected by the malachite green method at 630 nm
-
-
-
additional information
?
-
-
substrates are commercial sodium phosphate glass with 25-PP25-, 65-PP65-, or PPK-synthesized polyphosphate with 700-PP700-average number of residues. Released phosphate is detected by the malachite green method at 630 nm
-
-
-
additional information
?
-
-
contains an exopolyphosphatase, EC 3.6.1.11 and a guanosine pentaphosphate phosphohydrolase with long-chain exopolyphosphatase activity, EC 3.6.1.40
-
-
?
additional information
?
-
-
h-prune is the missing exopolyphosphatase in animals and support the hypothesis that the metastatic effects of h-prune are modulated by inorganic polyphosphates, which are increasingly recognized as critical regulators in cells
-
-
?
additional information
?
-
-
ATP, diphosphate and p-nitrophenyl phosphate are not substrates
-
-
?
additional information
?
-
the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed
-
-
-
additional information
?
-
-
the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed
-
-
-
additional information
?
-
the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed
-
-
-
additional information
?
-
the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed
-
-
-
additional information
?
-
the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed
-
-
-
additional information
?
-
the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed
-
-
-
additional information
?
-
the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed
-
-
-
additional information
?
-
the enzyme does not cleave ppGpp
-
-
?
additional information
?
-
-
the enzyme does not cleave ppGpp
-
-
?
additional information
?
-
no substrate: guanosine pentaphosphate. Enzyme hydrolyzes ATP and ADP, but lacks GTPase activities
-
-
?
additional information
?
-
-
no substrate: guanosine pentaphosphate. Enzyme hydrolyzes ATP and ADP, but lacks GTPase activities
-
-
?
additional information
?
-
the enzyme does not cleave ppGpp
-
-
?
additional information
?
-
no substrate: guanosine pentaphosphate. Enzyme hydrolyzes ATP and ADP, but lacks GTPase activities
-
-
?
additional information
?
-
-
Pseudomonas aeruginosa exopolyphosphatase catalyzes the hydrolysis of polyphosphates (polyP), producing polyphosphate_n-1 plus inorganic phosphate, but the exopolyphosphatase is also a polyphosphate:ADP phosphotransferase. 0.1 ml of enzyme and polyphosphate substrate in 50 mM Tris-HCl, pH 8.0, 80 mM KCl, and 5 mM MgCl2 are mixed with 0.4 ml of a solution with 2.5% (NH4)6Mo7O24 x (H2O)4 in 3 NH2SO4 and 0.4 ml of 2% ascorbic acid/2% hydrazine in 0.1 NH2SO4, and the solution is brought to a final volume of 1.2 ml with triple glass-distilled water. Quantification of free phosphate is performed after 30 min of incubation at 37°C through measurement of the absorbance at 820 nm
-
-
-
additional information
?
-
-
the soluble enzyme from cattle tick Rhipicephalus microplus is capable of hydrolysing polyphosphates, molecular docking assays of RmPPase with polyphosphates, and molecular modelling, overview
-
-
-
additional information
?
-
-
the recombinant enzyme rRmPPase, a inorganic diphosphatase (EC 3.6.1.1) from Rhipicephalus microplus, also shows exopolyphosphatase activity. It has a greater affinity, higher catalytic efficiency and increased cooperativity for sodium phosphate glass type 15 (polyP15) than for sodium tripolyphosphate (polyP3). Molecular docking study. PolyP3 binds close to the Mg2+ atoms in the catalytic region of the protein, participating in their coordination network, whereas polyP15 interactions involve negatively charged phosphate groups and basic amino acid residues, such as Lys56, Arg58, and Lys193. PolyP15 has a more favourable theoretical binding affinity than polyP3, thus supporting the kinetic data
-
-
-
additional information
?
-
-
ATP, diphosphate and p-nitrophenyl phosphate are not substrates
-
-
?
additional information
?
-
-
PPN1 splitting long polyphosphate chains to shorter ones
-
-
?
additional information
?
-
-
PPX1 splitting off phosphate from the end of the polyphosphate chain
-
-
?
additional information
?
-
enzyme is a bifunctional exo- and endopolyphosphatase, activities of EC 3.6.1.11 and EC 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenylphosphate
-
-
?
additional information
?
-
-
enzyme is a bifunctional exo- and endopolyphosphatase, activities of EC 3.6.1.11 and EC 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenylphosphate
-
-
?
additional information
?
-
Ppx1 has high exopolyphosphatase activity (EC 3.6.1.11), but no endopolyphosphatase activity (EC 3.6.1.10). The Ppx1 activity with guanosine tetraphosphate is nearly 80% of activity with long-chain polyphosphates. Ppx1 does not hydrolyze ATP and dATP. Exopolyphosphatases (polyphosphate phosphohydrolases, EC 3.6.1.11) cleave phosphate from the end of the polyphosphate chain
-
-
-
additional information
?
-
-
Ppx1 has high exopolyphosphatase activity (EC 3.6.1.11), but no endopolyphosphatase activity (EC 3.6.1.10). The Ppx1 activity with guanosine tetraphosphate is nearly 80% of activity with long-chain polyphosphates. Ppx1 does not hydrolyze ATP and dATP. Exopolyphosphatases (polyphosphate phosphohydrolases, EC 3.6.1.11) cleave phosphate from the end of the polyphosphate chain
-
-
-
additional information
?
-
Ppx1 has high exopolyphosphatase activity (EC 3.6.1.11), but no endopolyphosphatase activity (EC 3.6.1.10). The Ppx1 activity with guanosine tetraphosphate is nearly 80% of activity with long-chain polyphosphates. Ppx1 does not hydrolyze ATP and dATP. Exopolyphosphatases (polyphosphate phosphohydrolases, EC 3.6.1.11) cleave phosphate from the end of the polyphosphate chain
-
-
-
additional information
?
-
-
ATP, diphosphate and p-nitrophenyl phosphate are not substrates
-
-
?
additional information
?
-
the PPX1 does not hydrolyze organic triphosphates such as ATP, diphosphate or long-chain polyphosphates. PPX1 does not contain a cyclic-nucleotide specific phosphodiesterase activity
-
-
?
additional information
?
-
-
the PPX1 does not hydrolyze organic triphosphates such as ATP, diphosphate or long-chain polyphosphates. PPX1 does not contain a cyclic-nucleotide specific phosphodiesterase activity
-
-
?
additional information
?
-
the PPX1 does not hydrolyze organic triphosphates such as ATP, diphosphate or long-chain polyphosphates. PPX1 does not contain a cyclic-nucleotide specific phosphodiesterase activity
-
-
?
additional information
?
-
TbNH2 is an exopolyphosphatase with higher activity on short chain polyphosphates. TbNH2 can dephosphorylate ATP and ADP but with lower affinity than for polyphosphate. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). TbNH2 has a higher affinity for polyphosphate700 than for polyphosphate60. TbNH2 has activity to release the gamma and beta phosphates from ATP and ADP
-
-
-
additional information
?
-
TbNH2 is an exopolyphosphatase with higher activity on short chain polyphosphates. TbNH2 can dephosphorylate ATP and ADP but with lower affinity than for polyphosphate. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). TbNH2 has a higher affinity for polyphosphate700 than for polyphosphate60. TbNH2 has activity to release the gamma and beta phosphates from ATP and ADP
-
-
-
additional information
?
-
TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyphosphate of various chain sizes. The enzyme has a higher affinity for polyphosphate60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay
-
-
-
additional information
?
-
TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyphosphate of various chain sizes. The enzyme has a higher affinity for polyphosphate60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay
-
-
-
additional information
?
-
TbNH2 is an exopolyphosphatase with higher activity on short chain polyphosphates. TbNH2 can dephosphorylate ATP and ADP but with lower affinity than for polyphosphate. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). TbNH2 has a higher affinity for polyphosphate700 than for polyphosphate60. TbNH2 has activity to release the gamma and beta phosphates from ATP and ADP
-
-
-
additional information
?
-
TbNH2 is an exopolyphosphatase with higher activity on short chain polyphosphates. TbNH2 can dephosphorylate ATP and ADP but with lower affinity than for polyphosphate. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). TbNH2 has a higher affinity for polyphosphate700 than for polyphosphate60. TbNH2 has activity to release the gamma and beta phosphates from ATP and ADP
-
-
-
additional information
?
-
TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyphosphate of various chain sizes. The enzyme has a higher affinity for polyphosphate60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay
-
-
-
additional information
?
-
TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyphosphate of various chain sizes. The enzyme has a higher affinity for polyphosphate60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay
-
-
-
additional information
?
-
polyphosphates play a role in the parasite's osmoregulation, overview
-
-
?
additional information
?
-
-
polyphosphates play a role in the parasite's osmoregulation, overview
-
-
?
additional information
?
-
the enzyme prefers short-chain polyphosphates, substrate specificity, TcPPX is a processive enzyme and does not hydrolyze ATP, diphosphate, or 4-nitrophenyl phosphate, although it hydrolyzes guanosine 5'-tetraphosphate very efficiently, overview
-
-
?
additional information
?
-
-
the enzyme prefers short-chain polyphosphates, substrate specificity, TcPPX is a processive enzyme and does not hydrolyze ATP, diphosphate, or 4-nitrophenyl phosphate, although it hydrolyzes guanosine 5'-tetraphosphate very efficiently, overview
-
-
?
additional information
?
-
recombinant ZmPPX possesses exopolyphosphatase activity against a synthetic poly-P substrate
-
-
-
additional information
?
-
-
recombinant ZmPPX possesses exopolyphosphatase activity against a synthetic poly-P substrate
-
-
-
additional information
?
-
recombinant ZmPPX possesses exopolyphosphatase activity against a synthetic poly-P substrate
-
-
-
