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0.025 - 0.133
(25R)-3beta-hydroxycholest-5-en-27-oate
0.264 - 0.597
(phosphate)13-18
-
0.0011 - 0.0028
(phosphate)15
0.0069
(phosphate)25
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
-
0.0002 - 0.213
(phosphate)3
0.019 - 0.041
(phosphate)4
0.0022
(phosphate)45
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
-
0.0007 - 0.0036
(phosphate)65
-
0.0173
(polyphosphate)130
pH 6.8, 37°C
-
0.0059
(polyphosphate)14
pH 6.8, 37°C
-
0.0121
(polyphosphate)60
pH 6.8, 37°C
-
0.0035 - 1343
(Polyphosphate)n
0.028 - 0.08
adenosine 5'-tetraphosphate
0.1
adenosine-5'-tetraphosphate
-
-
0.0465
ADP
pH 7.4, 37°C, recombinant enzyme
0.0781
ATP
pH 7.4, 37°C, recombinant enzyme
0.0033
cAMP
pH 7.2, 30°C, recombinant enzyme
0.012 - 54.5
guanosine 5'-tetraphosphate
0.08
guanosine-5'-tetraphosphate
-
-
2.49
p-nitrophenyl phosphate
+/- 0.4
0.0272
pentasodium triphosphate
in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C
0.0035 - 12
Polyphosphate
75
polyphosphate 15
-
-
-
3.5
polyphosphate 208
-
-
-
1100
polyphosphate 3
-
-
-
0.3315
polyphosphate glass type 15
-
recombinant enzyme, pH 7.5, 30°C
-
0.01103 - 0.02083
polyphosphate25
0.7244
polyphosphate3
-
recombinant enzyme, pH 7.5, 30°C
-
0.00714 - 0.0236
polyphosphate45
-
0.0822 - 0.2005
polyphosphate60
-
0.00329 - 0.02517
polyphosphate65
-
0.1256 - 0.1494
polyphosphate700
-
0.0013 - 0.03067
polyphosphate75
-
0.0022
sodium phosphate glass type 15
-
in 50 mM Tris-HCl buffer (pH 7.2), at 28°C
-
0.11
Tetraphosphate
in 50 mM PIPES, pH 6.8 containing 25 mM KCl and 2 mM MgCl2
0.0002 - 0.058
Triphosphate
0.14 - 0.41
tripolyphosphate
additional information
additional information
-
0.025
(25R)-3beta-hydroxycholest-5-en-27-oate
polyphosphate 208, in the presence of 2.5 mM Mg2+
0.133
(25R)-3beta-hydroxycholest-5-en-27-oate
polyphosphate 15, in the presence of 2.5 mM Mg2+
0.264
(phosphate)13-18
pH 6.5, 25°C
-
0.597
(phosphate)13-18
pH 6.5, 25°C
-
0.0011
(phosphate)15
-
nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5
0.0028
(phosphate)15
-
mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5
0.0002
(phosphate)3
-
mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5
0.0007
(phosphate)3
-
nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5
0.0012
(phosphate)3
-
D106A mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.0022
(phosphate)3
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.0026
(phosphate)3
-
H107N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.0066
(phosphate)3
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.015
(phosphate)3
-
H108N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.046
(phosphate)3
-
R128H mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.0977
(phosphate)3
pH 6.5, 25°C
0.213
(phosphate)3
pH 6.5, 25°C
0.019
(phosphate)4
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.041
(phosphate)4
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.0007
(phosphate)65
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
-
0.0009
(phosphate)65
-
nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5
-
0.0036
(phosphate)65
-
mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5
-
0.0035
(Polyphosphate)n
-
n = 208, pH 7.2, 30°C
0.02
(Polyphosphate)n
+/- 0.003
0.025
(Polyphosphate)n
n = 208
0.075
(Polyphosphate)n
-
n = 15, pH 7.2, 30°C
0.133
(Polyphosphate)n
n = 15
1.1
(Polyphosphate)n
-
n = 3, pH 7.2, 30°C
26.8
(Polyphosphate)n
-
n = 4, pH 7.2
28.1
(Polyphosphate)n
-
n = 3, pH 7.2
39.1
(Polyphosphate)n
-
n = 15, pH 7.2
57.6
(Polyphosphate)n
-
n = 75, pH 7.2
1343
(Polyphosphate)n
-
n = 45, pH 7.2
0.028
adenosine 5'-tetraphosphate
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.037
adenosine 5'-tetraphosphate
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.08
adenosine 5'-tetraphosphate
-
at pH 4.8, 50 mM sodium acetate, 5 mM CoCl2
0.012
guanosine 5'-tetraphosphate
pH 7.5, 30°C
0.041
guanosine 5'-tetraphosphate
-
2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.099
guanosine 5'-tetraphosphate
-
2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA
0.242
guanosine 5'-tetraphosphate
pH 6.5, 25°C
0.335
guanosine 5'-tetraphosphate
pH 6.5, 25°C
54.5
guanosine 5'-tetraphosphate
-
pH 7.2
0.0039
polyP10
-
-
0.0092
polyP10
-
exopolyphosphatase II
0.0238
polyP10
-
exopolyphosphatase I
0.011
polyP15
-
-
0.0012
polyP208
-
-
0.0035
Polyphosphate
-
208 phosphate residues, pH 7.2
0.075
Polyphosphate
-
15 phosphate residues, pH 7.2
1.1
Polyphosphate
-
3 phosphate residues, pH 7.2
5
Polyphosphate
-
pH 7.2, 25°C, recombinant wild-type enzyme
6
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant H149N
7.5
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant H148N
9
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant N35H
12
Polyphosphate
-
pH 7.2, 25°C, recombinant mutant D127N
0.01103
polyphosphate25
-
pH 8.0, 37°C, full-length enzyme
0.02083
polyphosphate25
-
pH 8.0, 37°C, truncated enzyme
0.00714
polyphosphate45
-
pH 8.0, 37°C, full-length enzyme
-
0.0236
polyphosphate45
-
pH 8.0, 37°C, truncated enzyme
-
0.0822
polyphosphate60
pH 7.4, 37°C, recombinant enzyme
-
0.2005
polyphosphate60
pH 7.4, 37°C, recombinant enzyme
-
0.00329
polyphosphate65
-
pH 8.0, 37°C, full-length enzyme
-
0.02517
polyphosphate65
-
pH 8.0, 37°C, truncated enzyme
-
0.1256
polyphosphate700
pH 7.4, 37°C, recombinant enzyme
-
0.1494
polyphosphate700
pH 7.4, 37°C, recombinant enzyme
-
0.0013
polyphosphate75
-
pH 8.0, 37°C, full-length enzyme
-
0.03067
polyphosphate75
-
pH 8.0, 37°C, truncated enzyme
-
0.0002
Triphosphate
-
in 50 mM Tris-HCl buffer (pH 7.2), at 28°C
0.04
Triphosphate
in 50 mM PIPES, pH 6.8 containing 25 mM KCl and 2 mM MgCl2
0.058
Triphosphate
pH 7.5, 30°C
0.14
tripolyphosphate
-
-
0.39
tripolyphosphate
-
-
0.41
tripolyphosphate
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
steady-state kinetic analysis
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
kinetic analysis of recombinant wild-type and truncated mutant enzymes, exopolyphosphatase activity and polyphosphate:ADP phosphotransferase activity (EC 2.7.4.), overview
-