3.5.99.2: aminopyrimidine aminohydrolase

This is an abbreviated version!
For detailed information about aminopyrimidine aminohydrolase, go to the full flat file.

Reaction

Synonyms

HP1287, TenA, TenA_C, Thi20, Thi20p, thiaminase, thiaminase II, thiaminase type II

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.99 In other compounds

                3.5.99.2 aminopyrimidine aminohydrolase

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Results	in table
4	Activating Compound
10158	AA Sequence
1	CAS Registry Number
9	Cloned(Commentary)
6	Crystallization (Commentary)
8	General Information
18	Inhibitors
2	kcat/KM [mM/s]
8	KM Value [mM]
1	Localization
8	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
23	Organism
6	Pathway
14	PDB ID
9	pH Optimum
1	pH Range
2	Protein Variants
11	Purification (Commentary)
2	Reaction
2	Reaction Type
23	Reference
7	Specific Activity [micromol/min/mg]
43	Substrates and Products (Substrate)
11	Subunits
24	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
4	Temperature Stability [°C]
6	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 3.5.99.2 - aminopyrimidine aminohydrolase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.0046

Staphylococcus aureus

-

in 100 mM Tris-HCl, pH 7.5, at 37°C

713350

0.091

Bacillus subtilis

P25052

with oxothiamin as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine

733301

0.162

Bacillus subtilis

P25052

with thiamine as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine

733301

0.205

Bacillus subtilis

P25052

with oxythiamin as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine

733301

0.292

Bacillus subtilis

P25052

with 4-amino-5-aminomethyl-2-methylpyrimidine as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine

733301

additional information

2 entries

additional information

Alosa pseudoharengus

-

mean thiaminase activity in the alewives studied increases from 6.9 to 16.0 nmol/min/g from the time of their collection in Cayuga lake to the start of laboratory experiments 1.5-2.5 years later, the latter value is more than twice that of previously reported levels of thiaminase activity from alewives collected in the wild, data suggest that the variability in thiaminase activity is not related to stress from salt reduction or food limitation

698533

additional information

Alosa pseudoharengus

-

whole body activity is measured, no significant diferrences in thiaminase activity are found between the control fishes and the alewives which are exposed to food limitation and reduced salt content in the water

698533