3.5.99.2: aminopyrimidine aminohydrolase

This is an abbreviated version!
For detailed information about aminopyrimidine aminohydrolase, go to the full flat file.

Reaction

Synonyms

HP1287, TenA, TenA_C, Thi20, Thi20p, thiaminase, thiaminase II, thiaminase type II

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.99 In other compounds

                3.5.99.2 aminopyrimidine aminohydrolase

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Results	in table
4	Activating Compound
10158	AA Sequence
1	CAS Registry Number
9	Cloned(Commentary)
6	Crystallization (Commentary)
8	General Information
18	Inhibitors
2	kcat/KM [mM/s]
8	KM Value [mM]
1	Localization
8	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
23	Organism
6	Pathway
14	PDB ID
9	pH Optimum
1	pH Range
2	Protein Variants
11	Purification (Commentary)
2	Reaction
2	Reaction Type
23	Reference
7	Specific Activity [micromol/min/mg]
43	Substrates and Products (Substrate)
11	Subunits
24	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
4	Temperature Stability [°C]
6	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 3.5.99.2 - aminopyrimidine aminohydrolase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3

Saccharomyces cerevisiae

Q08224

physiological substrate, hydrolyzed more than two times faster than thiamine. Thiaminase II activity of Thi20p is involved in the thiamine salvage pathway by catalyzing the hydrolysis of 4-amino-5-hydroxymethyl-2-methylpyrimidine precursors in Saccharomyces cerevisiae

686824

-

-

?

4-amino-5-aminomethyl-2-methylpyrimidine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3

3 entries

oxothiamin + H2O

?

2 entries

oxythiamin + H2O

?

2 entries

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

11 entries

additional information

?

-

3 entries

4-amino-5-aminomethyl-2-methylpyrimidine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3

Bacillus subtilis

P25052

-

733301

-

-

?

4-amino-5-aminomethyl-2-methylpyrimidine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3

Bacillus subtilis 168

P25052

-

733301

-

-

?

4-amino-5-aminomethyl-2-methylpyrimidine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3

Staphylococcus aureus

-

-

733003

-

-

?

oxothiamin + H2O

?

Bacillus subtilis

P25052

-

733301

-

-

?

oxothiamin + H2O

?

Bacillus subtilis 168

P25052

-

733301

-

-

?

oxythiamin + H2O

?

Bacillus subtilis

P25052

-

733301

-

-

?

oxythiamin + H2O

?

Bacillus subtilis 168

P25052

-

733301

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Alosa pseudoharengus

-

-

698533

-

-

r

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Aneurinibacillus aneurinilyticus

-

-

246756, 246757, 246758, 246759, 246760, 246761, 246762

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Bacillus subtilis

P25052

-

733301

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Bacillus subtilis 168

P25052

-

733301

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Candida sp. (in: Saccharomycetales)

-

-

246760

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Geotrichum candidum

-

-

246760

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

moulds

-

-

246759

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Saccharomyces cerevisiae

-

-

246755

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Saccharomyces cerevisiae

Q08224

-

661316

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Staphylococcus aureus

-

-

733003

-

-

?

thiamine + H2O

4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole

Trichosporon aneurinolyticum

-

-

246760

-

-

?

additional information

?

-

Bacillus subtilis

P25052

no activity with N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine, thiamin monophosphate, thiamin diphosphate, thiamin disulfide, desthiothiamin, and thiochrome

733301

-

-

?

additional information

?

-

Bacillus subtilis 168

P25052

no activity with N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine, thiamin monophosphate, thiamin diphosphate, thiamin disulfide, desthiothiamin, and thiochrome

733301

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

THI20 is a trifunctional enzyme that is composed of a ThiD-like N-terminal domain that catalyzes the phosphorylation of 4-amino-5-hydroxymethyl-2-methylpyrimidine to 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate and of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate to 4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate, and a TenA-like C-terminal domain with thiaminase activity

718477

-

-

?