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3.5.99.2: aminopyrimidine aminohydrolase

This is an abbreviated version!
For detailed information about aminopyrimidine aminohydrolase, go to the full flat file.

Word Map on EC 3.5.99.2

Reaction

4-amino-5-aminomethyl-2-methylpyrimidine
+
H2O
=
4-Amino-5-hydroxymethyl-2-methylpyrimidine
+
NH3

Synonyms

HP1287, TenA, TenA_C, Thi20, Thi20p, thiaminase, thiaminase II, thiaminase type II

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.99 In other compounds
                3.5.99.2 aminopyrimidine aminohydrolase

Crystallization

Crystallization on EC 3.5.99.2 - aminopyrimidine aminohydrolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
dialyzing the purified enzyme solution against 52% saturated ammonium sulfate solution, two weeks
-
hanging drop vapour diffusion method
vapor diffusion method, using 0.1 m Tris (pH 8.5), 1.1 M lithium sulfate
purified recombinant C-terminally His6-tagged THI20, hanging drop vapour diffusion method, 10 mg/ml protein in 10 mM Tris buffer, pH 7.6, with 30 mM NaCl, is mixed with 10-14% PEG 8000, 0.2 M calcium acetate, and 0.1 M imidazole, pH 7.5, microseeding, 2-3 days, X-ray diffraction structure determination and analysis at 2.68 A resolution, molecular replacement, modelling
-
hanging drop vapor diffusion method
-
purified recombinant C-terminally Strep-tagged TenA, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein in 100 mM Tris-HCl buffer pH 8.0, is mixed with 0.002 l of reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris-HCl pH 8.5 and 28% w/v PEG 3350, 20°C, 7 days, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement, modelling
-