3.5.99.2: aminopyrimidine aminohydrolase

This is an abbreviated version!
For detailed information about aminopyrimidine aminohydrolase, go to the full flat file.

Reaction

Synonyms

HP1287, TenA, TenA_C, Thi20, Thi20p, thiaminase, thiaminase II, thiaminase type II

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.99 In other compounds

                3.5.99.2 aminopyrimidine aminohydrolase

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Results	in table
4	Activating Compound
10158	AA Sequence
1	CAS Registry Number
9	Cloned(Commentary)
6	Crystallization (Commentary)
8	General Information
18	Inhibitors
2	kcat/KM [mM/s]
8	KM Value [mM]
1	Localization
8	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
23	Organism
6	Pathway
14	PDB ID
9	pH Optimum
1	pH Range
2	Protein Variants
11	Purification (Commentary)
2	Reaction
2	Reaction Type
23	Reference
7	Specific Activity [micromol/min/mg]
43	Substrates and Products (Substrate)
11	Subunits
24	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
4	Temperature Stability [°C]
6	Turnover Number [1/s]

Cloned

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Cloned on EC 3.5.99.2 - aminopyrimidine aminohydrolase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli BL21(DE3) cells

Helicobacter pylori

O25874

711977

expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells

Bacillus subtilis

P25052

733301

expressed in Escherichia coli BLR (DE3) cells

Staphylococcus aureus

-

713350, 733003

expressed in Escherichia coli strain BL21(D3)

Bacillus subtilis

P25052

667651

expression in Escherichia coli

Saccharomyces cerevisiae

Q08224

661316

expression of C-terminally His6-tagged THI20 in Escherichia coli strain BL21 (DE3)

Saccharomyces cerevisiae

-

718477

expression of C-terminally Strep-tagged TenA in Escherichia coli strain BL21(DE3)

Staphylococcus aureus

-

718509

overexpression in Escherichia coli

Saccharomyces cerevisiae

Q08224

686824