3.5.5.7: Aliphatic nitrilase
This is an abbreviated version!
For detailed information about Aliphatic nitrilase, go to the full flat file.
Word Map on EC 3.5.5.7
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3.5.5.7
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rhodococcus
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enantioselectivity
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erythropolis
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synthesis
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dinitriles
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acetamide
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rhodochrous
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industry
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mononitriles
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microbacterium
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arylaliphatic
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nitrile-degrading
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environmental protection
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biotechnology
- 3.5.5.7
- rhodococcus
-
enantioselectivity
- erythropolis
- synthesis
-
dinitriles
- acetamide
- rhodochrous
- industry
-
mononitriles
- microbacterium
-
arylaliphatic
-
nitrile-degrading
- environmental protection
- biotechnology
Reaction
Synonyms
BjNIT3397, cyanoalanine hydratase/nitrilase, More, NIT, NIT1, Nit4, NitA, nitC1, nitC2, NitPf5, nitrilase, nitrile hydratase/amidase, PaCNit, REH16
ECTree
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Reaction
Reaction on EC 3.5.5.7 - Aliphatic nitrilase
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R-CN + 2 H2O = R-COOH + NH3
enzyme structure and reaction mechanism
R-CN + 2 H2O = R-COOH + NH3
enzyme structure, covalent mechanism, formation of an enzyme-thioimidate intermediate rather than addition of water to the nitrile
R-CN + 2 H2O = R-COOH + NH3
preferentially hydrolyses aliphatic nitriles, some of which are apparently not substrates for other known nitrilases, EC 3.5.5.1. Substrates include crotonitrile, acrylnitrile and glutaronitrile
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R-CN + 2 H2O = R-COOH + NH3
hydrolysis of substrates probably involves the conjugated pi-electron system of the aromatic ring of substrate or Tyr-142 as an electron acceptor
R-CN + 2 H2O = R-COOH + NH3
preferentially hydrolyses aliphatic nitriles, some of which are apparently not substrates for other known nitrilases, EC 3.5.5.1. Substrates include crotonitrile, acrylnitrile and glutaronitrile
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