3.5.5.7: Aliphatic nitrilase

This is an abbreviated version!
For detailed information about Aliphatic nitrilase, go to the full flat file.

Word Map on EC 3.5.5.7

3.5.5.7

rhodococcus

enantioselectivity

erythropolis

synthesis

dinitriles

acetamide

rhodochrous

industry

mononitriles

microbacterium

arylaliphatic

nitrile-degrading

environmental protection

biotechnology

Reaction

+ 2 H2O =

Synonyms

BjNIT3397, cyanoalanine hydratase/nitrilase, More, NIT, NIT1, Nit4, NitA, nitC1, nitC2, NitPf5, nitrilase, nitrile hydratase/amidase, PaCNit, REH16

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.5 In nitriles

3.5.5.7 Aliphatic nitrilase

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Results	in table
18	Activating Compound
740	AA Sequence
20	Application
2	CAS Registry Number
19	Cloned(Commentary)
2	Crystallization (Commentary)
62	General Information
1	IC50 Value
114	Inhibitors
19	kcat/KM [mM/s]
75	KM Value [mM]
4	Localization
13	Metals/Ions
41	Molecular Weight [Da]
1	Organic Solvent Stability
226	Organism
5	Pathway
23	pH Optimum
8	pH Range
3	pH Stability
8	pI Value
65	Protein Variants
15	Purification (Commentary)
6	Reaction
3	Reaction Type
140	Reference
2	Source Tissue
62	Specific Activity [micromol/min/mg]
397	Substrates and Products (Substrate)
36	Subunits
29	Synonyms
1	Systematic Name
23	Temperature Optimum [°C]
8	Temperature Range [°C]
23	Temperature Stability [°C]
59	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 3.5.5.7 - Aliphatic nitrilase

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138000

Pseudomonas fluorescens

gel filtration

29800

30000

2 * 30000, SDS-PAGE

30070

SDS-PAGE

31000

Pannonibacter carbonis

SDS-PAGE

756271

33000

Pseudomonas fluorescens

Q4KCL8

4 * 33000, SDS-PAGE

695831

36320

Bradyrhizobium sp.

calculated from amino acid sequence

712870

36350

Synechococcus elongatus

A0A0H3K0Y4

calculated from amino acid sequence

712870

36550

Pseudomonas syringae pv. syringae

Q500U1

calculated from amino acid sequence

712870

37000

Fusarium proliferatum

A0A0E3D8K7

x * 37000, SDS-PAGE

733919

37970

Methylibium petroleiphilum

A2SEG6

calculated from amino acid sequence

38000

390000

40000

40190

Rhodococcus rhodochrous

calculated from amino acid sequence

712870

41000

6 entries

42275

Rhodococcus rhodochrous

x * 42275, calculation from nucleotide sequence

32505

42280

Rhodococcus rhodochrous

calculated from amino acid sequence

712870

43000

Acinetobacter sp.

14 * 41000 or 14 * 43000

712260

560000

Cereibacter sphaeroides

gel filtration

570000

580000

59800

gel filtration

60000

604000

Rhodococcus rhodochrous

equilibrium sedimentation

650000

700000

gel filtration