Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.95 - 9.16
2-butenenitrile
0.4 - 73.1
3-Cyanopyridine
11.26
acetonitrile
-
pH 7.0, 37°C, partially purified enzyme
1.14 - 23.3
acrylonitrile
0.688 - 10.28
adiponitrile
0.47 - 1.85
m-tolunitrile
0.75
potassium cyanide
-
pH 7.0, 37°C, partially purified enzyme
43.9
Propionitrile
pH 7.0, 30°C
25.3
sebaconitrile
pH 7.0, 30°C
17.9
Succinonitrile
pH 7.0, 30°C
additional information
additional information
-
kinetics
-
7.95
2-butenenitrile
wild type enzyme, at pH 7.0 and 30°C
9.16
2-butenenitrile
mutant enzyme W146F, at pH 7.0 and 30°C
0.82
2-Cyanopyridine
mutant Y142A, pH 7.5, 25°C
1.13
2-Cyanopyridine
mutant enzyme W146Y, at pH 7.0 and 30°C
1.25
2-Cyanopyridine
mutant K9A, pH 7.5, 25°C
1.26
2-Cyanopyridine
mutant enzyme W146F, at pH 7.0 and 30°C
1.3
2-Cyanopyridine
mutant enzyme W146H, at pH 7.0 and 30°C
1.47
2-Cyanopyridine
wild type enzyme, at pH 7.0 and 30°C
1.5
2-Cyanopyridine
mutant L79A, pH 7.5, 25°C
1.53
2-Cyanopyridine
wild-type, pH 7.5, 25°C
1.56
2-Cyanopyridine
mutant Y142F, pH 7.5, 25°C
3.7 - 5
2-Cyanopyridine
mutant Y142S, pH 7.5, 25°C
0.4
3-Cyanopyridine
-
pH 7.0, 30°C
3.16
3-Cyanopyridine
mutant enzyme W146Y, at pH 7.0 and 30°C
3.2
3-Cyanopyridine
wild type enzyme, at pH 7.0 and 30°C
3.94
3-Cyanopyridine
mutant enzyme W146H, at pH 7.0 and 30°C
4.41
3-Cyanopyridine
mutant enzyme W146F, at pH 7.0 and 30°C
7.63
3-Cyanopyridine
pH 7.5, temperature not specified in the publication
10.31
3-Cyanopyridine
-
pH 7.2, 30°C
73.1
3-Cyanopyridine
at pH 7.0 and 30°C
1.14
acrylonitrile
-
-
5.88
acrylonitrile
-
pH 7.0, 37°C, partially purified enzyme
23.3
acrylonitrile
pH 7.0, 30°C
0.688
adiponitrile
mutant enzyme Y142F
0.69
adiponitrile
mutant Y142F, pH 7.5, 25°C
0.85
adiponitrile
mutant K9A, pH 7.5, 25°C
0.92
adiponitrile
mutant L79A, pH 7.5, 25°C
0.93
adiponitrile
wild-type, pH 7.5, 25°C
0.933
adiponitrile
wild type enzyme
10.28
adiponitrile
-
pH 7.0, 37°C, partially purified enzyme
0.49
Benzonitrile
mutant Y142V, pH 7.5, 25°C
0.91
Benzonitrile
mutant L79A, pH 7.5, 25°C
0.98
Benzonitrile
mutant enzyme W146Y, at pH 7.0 and 30°C
1.02
Benzonitrile
mutant K9A, pH 7.5, 25°C
1.07
Benzonitrile
wild-type, pH 7.5, 25°C
1.21
Benzonitrile
wild type enzyme, at pH 7.0 and 30°C
1.26
Benzonitrile
mutant enzyme W146F, at pH 7.0 and 30°C
1.33
Benzonitrile
mutant enzyme W146H, at pH 7.0 and 30°C
1.55
Benzonitrile
at pH 8.0 and 37°C
1.58
Benzonitrile
mutant Y142A, pH 7.5, 25°C
1.82
Benzonitrile
mutant Y142S, pH 7.5, 25°C
3.45
Benzonitrile
mutant Y142F, pH 7.5, 25°C
12.27
Benzonitrile
-
pH 7.0, 37°C, partially purified enzyme
23.8
Benzonitrile
pH 7.0, 30°C
2 - 3
Fumaronitrile
pH 7.0, 30°C
9.01
Fumaronitrile
wild type enzyme, at pH 7.0 and 30°C
9.48
Fumaronitrile
-
pH 7.4, 80°C
9.48
Fumaronitrile
-
in 20 mM potassium phosphate, pH 7.4, with 1 mM dithiothreitol, at 80°C
10.77
Fumaronitrile
mutant enzyme W146Y, at pH 7.0 and 30°C
11.06
Fumaronitrile
mutant enzyme W146H, at pH 7.0 and 30°C
11.45
Fumaronitrile
mutant enzyme W146F, at pH 7.0 and 30°C
1.15
glutaronitrile
mutant L79A, pH 7.5, 25°C
1.19
glutaronitrile
mutant K9A, pH 7.5, 25°C
1.24
glutaronitrile
wild-type, pH 7.5, 25°C
1.241
glutaronitrile
wild type enzyme
1.56
glutaronitrile
mutant enzyme Y142F
1.56
glutaronitrile
mutant Y142F, pH 7.5, 25°C
24
glutaronitrile
pH 7.0, 30°C
47
glutaronitrile
at 30°C in 50 mM sodium phosphate buffer (pH 7.5) and 1 mM dithiothreitol
0.47
m-tolunitrile
mutant Y142V, pH 7.5, 25°C
0.61
m-tolunitrile
mutant Y142A, pH 7.5, 25°C
1.19
m-tolunitrile
mutant Y142F, pH 7.5, 25°C
1.49
m-tolunitrile
mutant Y142S, pH 7.5, 25°C
1.62
m-tolunitrile
mutant K9A, pH 7.5, 25°C
1.79
m-tolunitrile
mutant L79A, pH 7.5, 25°C
1.85
m-tolunitrile
wild-type, pH 7.5, 25°C
3.47
Malononitrile
-
pH 7.4, 80°C
3.47
Malononitrile
-
in 20 mM potassium phosphate, pH 7.4, with 1 mM dithiothreitol, at 80°C
3.5.5.7 Aliphatic nitrilase
results (
results (
top
