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5,5'-dithiobis(2-nitrobenzoate)
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complete inhibition at 5 mM
BaCl2
-
5 mM, 35% inhibition
CaCl2
-
5 mM, 34% inhibition
chloroform
5%, about 90% inhibition
Cr3+
-
5 mM, 40% inhibition; 60% residual activity at 5 mM
Dichloromethane
about 30% residual activity at 5% (v/v)
dimethyl sulfoxide
-
15% (v/v),about 35% inhibition
dithiothreitol
-
5 mM, 50% inhibition
DMSO
5%, about 45% inhibition
DTNB
-
5 mM, complete inhibition
DTT
-
5 mM, 37% inhibition
ether
5%, about 70% inhibition
ethyl acetate
5%, about 85% inhibition
ethylene glycol
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80% inhibition at 50%; 80% inhibition at 50% ethylene glycol
FeCl3
-
5 mM, 23% inhibition
FeSO4
-
5 mM, 47% inhibition
glycerin
-
5% (v/v), 52% inhibition
glycerol
5%, about 40% inhibition
hexane
about 20% residual activity at 5% (v/v)
HgCl2
-
inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione
iodacetamide
-
complete inhibition at 5 mM
iodacetate
-
complete inhibition at 5 mM
iodoacetamide
-
5 mM, complete inhibition
iodoacetate
-
5 mM, complete inhibition
KCl
-
5 mM, 58% inhibition
L-Cys
-
5 mM, 27% inhibition
L-cysteine
1 mM, 6% inhibition
L-glutathione
1 mM, 17% inhibition
Malononitrile
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concentrations above 12 mM have a strong inhibitory effect; strong inhibition at 12 mM
MgCl2
-
5 mM, 18% inhibition
MnCl2
-
5 mM, 50% inhibition
N-ethylmaleimide
0.1 mM, 48% residual activity
n-hexane
-
5% (v/v), 6% inhibition
NaCl
-
5 mM, 26% inhibition
NH3
-
inhibition partially reversed by DTT, 2-mercaptoethanol, or reduced glutathione
p-chloromercuribenzoate
-
complete inhibition at 1 mM
p-hydroxymercuribenzoate
-
1 mM, complete inhibition; complete inhibition at 1 mM
phenyl hydrazine
-
53% residual activity at 5 mM; 5 mM, 47% inhibition
PMSF
1 mM, 51% inhibition
Propanol
about 22% residual activity at 5% (v/v)
pyridoxal 5'-phosphate
0.1 mM, 30% residual activity
tetrahydrofuran
-
10% (v/v), about 95% inhibition
Toluene
about 5% residual activity at 5% (v/v)
Tween-20
1 mM, 54% inhibition
Tween-80
1 mM, 57% inhibition
ZnCl2
-
5 mM, 32% inhibition
2-mercaptoethanol
-
5 mM, 29% inhibition
2-mercaptoethanol
1 mM, 25% inhibition
acetone
-
25% (v/v), about 40% inhibition
acetone
-
10%, complete inhibition; complete inhibition at 10% acetone
acetone
5%, about 60% inhibition
Ag+
1% residual activity at 5 mM
Ag+
-
5 mM, 94% inhibition
Ag+
complete inhibition at 1 mM
Ag+
-
1 mM, complete inhibition; complete inhibition at 1 mM
Ag+
5% residual activity at 1 mM
Ba2+
about 55% residual activity at 1 mM
Ba2+
1 mM, 18% inhibition
Ca2+
60% residual activity at 5 mM
Ca2+
-
5 mM, 97% inhibition
Ca2+
-
1 mM, 20% inhibition; 75% residual activity at 5 mM
Co2+
75% residual activity at 5 mM
Co2+
-
5 mM, 15% inhibition
Co2+
about 65% residual activity at 1 mM
Co2+
1 mM, 8% residual activity
Co2+
1 mM, 21% inhibition
Cu2+
43% residual activity at 5 mM
Cu2+
-
5 mM, 70% inhibition
Cu2+
about 75% residual activity at 1 mM
Cu2+
1 mM, no residual activity
Cu2+
1 mM, 85% inhibition
EDTA
63% residual activity at 5 mM
EDTA
1 mM, 17% inhibition
EDTA
84% residual activity at 1 mM
ethanol
-
25% (v/v), about 20% inhibition
ethanol
about 55% residual activity at 5% (v/v)
ethanol
-
57% inhibition at 30%, complete inhibition at 50%; complete inhibition at 50% ethanol
ethanol
5%, about 10% inhibition
Fe2+
44% residual activity at 5 mM
Fe2+
-
5 mM, 51% inhibition
Fe2+
-
40% residual activity at 5 mM; 5 mM, 60% inhibition
Fe2+
72% residual activity at 1 mM
Hg2+
1 mM, 10% residual activity
Hg2+
-
1 mM, complete inhibition; complete inhibition at 1 mM
Isopropanol
-
25% (v/v), about 10% inhibition
Isopropanol
5%, about 30% inhibition
Li+
-
5 mM, 37% inhibition
Li+
about 80% residual activity at 1 mM
methanol
-
25% (v/v), about 20% inhibition
methanol
about 75% residual activity at 5% (v/v)
methanol
-
complete inhibition at 30%; complete inhibition at 30% methanol
methanol
5%, about 30% inhibition
Mg2+
93% residual activity at 5 mM
Mg2+
-
5 mM, 31% inhibition
Mn2+
-
5 mM, 61% inhibition
Mn2+
1 mM, 65% inhibition
Ni2+
87% residual activity at 5 mM
Ni2+
-
5 mM, 47% inhibition
Ni2+
about 60% residual activity at 1 mM
Ni2+
1 mM, 60% inhibition
PCMB
-
1 mM, complete inhibition
SDS
-
5% (v/v), 37% inhibition
Triton X-100
-
5% (v/v), 14% inhibition
Triton X-100
1 mM, 65% inhibition
Zn2+
19% residual activity at 5 mM
Zn2+
-
5 mM, 47% inhibition
Zn2+
1 mM, no residual activity
Zn2+
1 mM, 54% inhibition
additional information
-
minimal inhibition concentration MIC values of nitrile and amide substrates, the values lay between 150 mM and 2200 mM, overview
-
additional information
-
EDTA and NaN3 have no influence on activity
-
additional information
Nit1 is not or very weakly affected by Na+, Ca2+, Mg2+, and Fe3+
-
3.5.5.7 Aliphatic nitrilase
results (
results (
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