3.5.5.7: Aliphatic nitrilase

This is an abbreviated version!
For detailed information about Aliphatic nitrilase, go to the full flat file.

Word Map on EC 3.5.5.7

3.5.5.7

rhodococcus

enantioselectivity

erythropolis

synthesis

dinitriles

acetamide

rhodochrous

industry

mononitriles

microbacterium

arylaliphatic

nitrile-degrading

environmental protection

biotechnology

Reaction

+ 2 H2O =

Synonyms

BjNIT3397, cyanoalanine hydratase/nitrilase, More, NIT, NIT1, Nit4, NitA, nitC1, nitC2, NitPf5, nitrilase, nitrile hydratase/amidase, PaCNit, REH16

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.5 In nitriles

3.5.5.7 Aliphatic nitrilase

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Results	in table
18	Activating Compound
740	AA Sequence
20	Application
2	CAS Registry Number
19	Cloned(Commentary)
2	Crystallization (Commentary)
62	General Information
1	IC50 Value
114	Inhibitors
19	kcat/KM [mM/s]
75	KM Value [mM]
4	Localization
13	Metals/Ions
41	Molecular Weight [Da]
1	Organic Solvent Stability
226	Organism
5	Pathway
23	pH Optimum
8	pH Range
3	pH Stability
8	pI Value
65	Protein Variants
15	Purification (Commentary)
6	Reaction
3	Reaction Type
140	Reference
2	Source Tissue
62	Specific Activity [micromol/min/mg]
397	Substrates and Products (Substrate)
36	Subunits
29	Synonyms
1	Systematic Name
23	Temperature Optimum [°C]
8	Temperature Range [°C]
23	Temperature Stability [°C]
59	Turnover Number [1/s]

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Cloned on EC 3.5.5.7 - Aliphatic nitrilase

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Thermotoga maritima

Q9WYX6

757415

DNA sequence determination and analysis

Meyerozyma guilliermondii

655149

DNA sequence determination and analysis, overexpression in Escherichia coli

Acidovorax facilis

654298

expressed in Escherichia coli

2 entries

expressed in Escherichia coli BL21 (DE3) cells

Fusarium proliferatum

A0A0E3D8K7

733919

expressed in Escherichia coli BL21(DE3) cells

2 entries

expressed in Escherichia coli Rosetta (DE3) cells

Cereibacter sphaeroides

G5DDB2

735340

expressed in Escherichia coli strain ER2566

Rhodococcus rhodochrous

Q03217

685014

expression in Escherichia coli

6 entries

expression in Escherichia coli Rosetta2(DE3)

Bradyrhizobium japonicum

757441

overexpressed in Escherichia coli BL21(DE3)

Cupriavidus necator

756205

overproduction in Escherichia coli

Rhodococcus rhodochrous

32505