3.5.5.7: Aliphatic nitrilase

This is an abbreviated version!
For detailed information about Aliphatic nitrilase, go to the full flat file.

Word Map on EC 3.5.5.7

3.5.5.7

rhodococcus

enantioselectivity

erythropolis

synthesis

dinitriles

acetamide

rhodochrous

industry

mononitriles

microbacterium

arylaliphatic

nitrile-degrading

environmental protection

biotechnology

Reaction

+ 2 H2O =

Synonyms

BjNIT3397, cyanoalanine hydratase/nitrilase, More, NIT, NIT1, Nit4, NitA, nitC1, nitC2, NitPf5, nitrilase, nitrile hydratase/amidase, PaCNit, REH16

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.5 In nitriles

3.5.5.7 Aliphatic nitrilase

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Results	in table
18	Activating Compound
740	AA Sequence
20	Application
2	CAS Registry Number
19	Cloned(Commentary)
2	Crystallization (Commentary)
62	General Information
1	IC50 Value
114	Inhibitors
19	kcat/KM [mM/s]
75	KM Value [mM]
4	Localization
13	Metals/Ions
41	Molecular Weight [Da]
1	Organic Solvent Stability
226	Organism
5	Pathway
23	pH Optimum
8	pH Range
3	pH Stability
8	pI Value
65	Protein Variants
15	Purification (Commentary)
6	Reaction
3	Reaction Type
140	Reference
2	Source Tissue
62	Specific Activity [micromol/min/mg]
397	Substrates and Products (Substrate)
36	Subunits
29	Synonyms
1	Systematic Name
23	Temperature Optimum [°C]
8	Temperature Range [°C]
23	Temperature Stability [°C]
59	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.5.5.7 - Aliphatic nitrilase

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2-mercaptoethanol

activates at 1 mM

Benzonitrile

dithiothreitol

DTT

Pannonibacter carbonis

1 mM, 1.18fold activation

756271

EDTA

2 entries

ethylene glycol

Pyrococcus abyssi

20% increase of activity at 10% ethylene glycol

670792

glycerin

Pannonibacter carbonis

1% (v/v), 1.3fold activation

756271

Isopropanol

Pannonibacter carbonis

1% (v/v), 1.2fold activation

756271

L-cysteine

2 entries

L-glutathione

Pannonibacter carbonis

1 mM, 1.27fold activation

756271

n-hexane

Pannonibacter carbonis

1% (v/v), 1.1fold activation

756271

SDS

Pannonibacter carbonis

1% (v/v), 1.4fold activation

756271

Span-80

Pannonibacter carbonis

1% (v/v), 1.2fold activation

756271

additional information

Streptomyces sp.

there is no appreciable variation in the activity of enzyme at any concentration of glycerol

687941