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3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Word Map on EC 3.5.5.1

Reaction

A nitrile
+ 2 H2O =
a carboxylate
 +
NH3

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase

pH Range

pH Range on EC 3.5.5.1 - nitrilase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
pH 4.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity
5 - 10
5 - 9
5.5 - 7.8
highly active between pH 5.5 and 7.8
5.5 - 8
5.5 - 9.5
-
active within
6 - 10
6 - 11.5
-
pH 6.0: about 55% of maximal activity, immobilized enzyme, pH 11.5: about 50% of maximal activity
6 - 7.5
-
pH 6.0: about 60% of maximal activity, pH 7.5: about 50% of maximal activity
6 - 8
6 - 9
6 - 9.5
pH 6.0: about 65% of maximal activity, pH 9.5: about 65% of maximal activity
6.5 - 9.5
-
-
6.7
-
half-maximal activity
7 - 8
-
more than 80% activity between pH 7.0 and 8.0
7 - 8.6
the nitrilase activity was low at pH less 7.0 or pH above 8.6
7.2 - 8.5
-
-
7.8 - 9.1
-
optimal activity within
8
-
activity decreases sharp below and above
8.7
-
half-maximal activity