3.5.5.1: nitrilase
This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.
Word Map on EC 3.5.5.1
-
3.5.5.1
-
enantioselectivity
-
amidase
-
hydratase
-
rhodococcus
-
biocatalyst
-
synthesis
-
rhodochrous
-
benzonitrile
-
mandelonitrile
-
indole-3-acetic
-
alcaligenes
-
indole-3-acetonitrile
-
3-cyanopyridine
-
dinitriles
-
phenylacetonitrile
-
acrylonitrile
-
bromoxynil
-
r-mandelic
-
nhase
-
acidovorax
-
ozaenae
-
facilis
-
industry
-
iminodiacetic
-
gibberella
-
analysis
- 3.5.5.1
-
enantioselectivity
- amidase
-
hydratase
- rhodococcus
-
biocatalyst
- synthesis
- rhodochrous
- benzonitrile
- mandelonitrile
-
indole-3-acetic
- alcaligenes
- indole-3-acetonitrile
- 3-cyanopyridine
-
dinitriles
- phenylacetonitrile
- acrylonitrile
- bromoxynil
-
r-mandelic
- nhase
-
acidovorax
- ozaenae
- facilis
- industry
-
iminodiacetic
- gibberella
- analysis
Reaction
Synonyms
3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122
ECTree
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Substrates Products
Substrates Products on EC 3.5.5.1 - nitrilase
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REACTION DIAGRAM
(3S)-3-(2,4-difluorophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(2,4-difluorophenyl)-3-hydroxypropanoic acid + NH3
(3S)-3-(3-methoxyphenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(3-methoxyphenyl)-3-hydroxypropanoic acid + NH3
(3S)-3-(3-nitrophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(3-nitrophenyl)-3-hydroxypropanoic acid + NH3
(3S)-3-(4-bromophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-bromophenyl)-3-hydroxypropanoic acid + NH3
(3S)-3-(4-chlorophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-chlorophenyl)-3-hydroxypropanoic acid + NH3
(3S)-3-(4-cyanophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-cyanophenyl)-3-hydroxypropanoic acid + NH3
-
92% yield, 95% enantiomeric excess
-
-
?
(3S)-3-(4-fluorophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-fluorophenyl)-3-hydroxypropanoic acid + NH3
-
93% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(4-methoxyphenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-methoxyphenyl)-3-hydroxypropanoic acid + NH3
-
91% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(4-methylphenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-methylphenyl)-3-hydroxypropanoic acid + NH3
-
91% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(4-nitrophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-nitrophenyl)-3-hydroxypropanoic acid + NH3
-
87% yield, 97% enantiomeric excess
-
-
?
(3S)-3-hydroxy-3-phenylpropanenitrile + H2O
(3S)-3-hydroxy-3-phenylpropanoic acid + NH3
-
90% yield, 99% enantiomeric excess
-
-
?
(3S)-isobutylsuccinonitrile + H2O
(3S)-3-cyano-5-methyl hexanoic acid
-
specific for the (3S)-isomer
-
-
?
(methylthio)acetonitrile + H2O
2-(methylthio)acetic acid + NH3
-
-
-
?
(phenylthio)acetonitrile + H2O
2-(phenylthio)acetic acid + NH3
-
-
-
?
(R)-2-chloromandelonitrile + H2O
(R)-2-chloromandelic acid + (S)-2-chloromandelic acid + NH3
(S)-2-acetoxy-2-phenylacetonitrile + H2O
(S)-2-acetoxy-2-phenylacetic acid + (S)-2-acetoxy-2-phenylacetamide
1,2-phenylenediacetonitrile + H2O
1,2-phenylenediacetic acid + NH3
-
-
-
?
1,2-phenylenediacetonitrile + H2O
? + NH3
0.063% activity compared to iminodiacetonitrile
-
-
?
1,3-phenylenediacetonitrile + H2O
1,3-phenylenediacetic acid + NH3
-
-
-
?
1,4-dicyanobutane + H2O
? + NH3
27.1% activity compared to iminodiacetonitrile
-
-
?
1,4-phenylenediacetonitrile + H2O
1,4-phenylenediacetic acid + NH3
-
-
-
?
1-(cyanoacetyl)pyrrolidine + H2O
? + NH3
low activity
-
-
?
1-cyanocyclohexaneacetonitrile + H2O
1-cyanocyclohexaneacetic acid + NH3
-
-
-
?
2 benzonitrile + 3 H2O
benzoic acid + benzamide + NH3
-
100% activity
-
-
?
2,2-dimethylcyclopropyl cyanide + 2 H2O
2,2-dimethylcyclopropyl carboxylate + NH3
-
-
-
?
2,4-dichlorophenyl acetonitrile + 2 H2O
2,4-dichlorophenyl acetic acid + NH3
-
-
-
-
?
2,4-pyridinedicarbonitrile + H2O
2-cyanopyridine-4-carboxylic acid + NH3
-
7% activity compared to benzonitrile
-
-
?
2,6-pyridinedicarbonitrile + H2O
6-cyanopyridine-2-carboxylic acid + NH3
-
5% activity compared to benzonitrile
-
-
?
2-amino-4-methylbenzonitrile + 2 H2O
2-amino-4-methylbenzoic acid + NH3
-
-
-
-
?
2-amino-5-chloro-benzonitrile + 2 H2O
2-amino-5-chloro-benzoic acid + NH3
-
-
-
-
?
2-amino-6-chloro-benzonitrile + 2 H2O
2-amino-6-chloro-benzoic acid + NH3
-
good substrate, when the cells are induced by benzonitrile
-
-
?
2-amino-benzonitrile + H2O
2-aminobenzoic acid + NH3
-
88% of the activity as compared to acetonitrile
-
-
?
2-aminobutyronitrile + 2 H2O
2-aminobutyric acid + NH3
high activity
-
-
?
2-aminopropionitrile + H2O
2-aminopropionic acid + NH3
-
low activity
-
?
2-bromobenzonitrile + H2O
2-bromobenzoic acid + NH3
-
best substrate of strain NCIB11216 and strain NCIB11215
-
-
?
2-chloro-2-phenylacetonitrile + H2O
2-chloro-2-phenylacetic acid + 2-chloro-2-phenylacetamide
2-chloro-3-cyanopyridine + H2O
?
-
0.21% activity compared to 3-cyanopyridine
-
-
?
2-chlorobenzonitrile + H2O
2-chlorobenzoic acid + NH3
Pyrococcus sp. MC-FB
-
1% of the activity compared to 2-cyanopyridine
-
-
?
2-chlorophenylacetonitrile + H2O
?
-
38% of the activity as compared to benzoylacetonitrile
-
-
?
2-cyanopyridine + 2 H2O
2-pyridinecarboxylic acid + NH3
-
7% activity compared to benzonitrile
-
-
?
2-furanocarbonitrile + H2O
furan-2-carboxylic acid + NH3
-
best substrate
-
?
2-methylbutyronitrile + H2O
2-methylbutyric acid + NH3
-
-
-
?
2-methylglutaronitrile + 4 H2O
2-methylglutaric acid + 2 NH3
Pyrococcus sp. MC-FB
-
9% of the activity compared to 2-cyanopyridine
-
-
?
2-phenylacetonitrile + 2 H2O
2-phenylacetic acid + NH3
-
10.8% activity compared to benzonitrile
-
-
?
2-phenylacetonitrile + 2 H2O
phenylacetate + NH3
-
substrate for isozyme complex NIT4A/B2
-
-
?
3,4,5-trimethoxybenzonitrile + 2 H2O
3,4,5-trimethoxybenzoic acid + NH3
-
-
-
-
?
3-(2,4-dichlorophenyl)-3-hydroxypropanenitrile + H2O
(S)-3-(2,4-dichlorophenyl)-3-hydroxypropanoic acid + NH3
3-(2-chlorophenyl)-3-hydroxypropanenitrile + H2O
3-(2-chlorophenyl)-3-hydroxypropanoic acid + NH3
3-(2-chlorophenyl)pentanedinitrile + H2O
(3S)-3-(2-chlorophenyl)-4-cyanobutanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 5.1fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 74% enantiomeric excess by wild-type enzyme and with 97% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-(3-chlorophenyl)pentanedinitrile + H2O
(3S)-3-(3-chlorophenyl)-4-cyanobutanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 3.1fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 93% enantiomeric excess by wild-type enzyme and with 98% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-(4-bromophenyl)pentanedinitrile + H2O
(3S)-3-(4-bromophenyl)-4-cyanobutanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 18.6fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 97% enantiomeric excess by wild-type enzyme and with more than 99% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-(4-chlorophenyl)-3-hydroxypropanenitrile + H2O
(S)-3-(4-chlorophenyl)-3-hydroxypropanoic acid + NH3
3-(4-chlorophenyl)pentanedinitrile + H2O
(3S)-3-(4-chlorophenyl)-4-cyanobutanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 11.0fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 97% enantiomeric excess by wild-type enzyme and with 99% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-(4-fluorophenyl)-3-hydroxypropanenitrile + H2O
(S)-3-(4-fluorophenyl)-3-hydroxypropanoic acid + NH3
3-(4-fluorophenyl)pentanedinitrile + H2O
(3S)-4-cyano-3-(4-fluorophenyl)butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 8.8fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 99% enantiomeric excess by wild-type enzyme and with more than 99% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-(4-hydroxyphenyl)pentanedinitrile + H2O
(3S)-4-cyano-3-(4-hydroxyphenyl)butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 78fold higher than activity of wild-type enzyme
-
-
?
3-(4-hydroxyphenyl)propanenitrile + H2O
3-(4-hydroxyphenyl)propanoic acid + NH3
-
-
-
-
?
3-(4-methoxyphenyl)pentanedinitrile + H2O
(3S)-4-cyano-3-(4-methoxyphenyl)butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 12.8fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 98% enantiomeric excess by wild-type enzyme and with more than 99% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-(4-methylphenyl)pentanedinitrile + H2O
(3S)-4-cyano-3-(4-methylphenyl)butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 6.7fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 98% enantiomeric excess by wild-type enzyme and with 99% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-(4-tert-butylphenyl)pentanedinitrile + H2O
(3S)-3-(4-tert-butylphenyl)-4-cyanobutanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is2.3fold higher than activity of wild-type enzyme
-
-
?
3-aminobutyronitrile + H2O
3-aminobutyric acid + NH3
201% of the activity compared to benzonitrile
-
-
?
3-anilinoproprionitrile + 2 H2O
3-anilinopropionate + NH3
-
-
-
?
3-bromo-4-hydroxybenzonitrile + H2O
3-bromo-4-hydroxybenzoate + NH3
-
low activity
-
?
3-butenenitrile + H2O
3-butenoic acid + NH3
the enzyme displays a relatively high degree of specificity for 3-butenenitrile. Helical twist and substrate size correlate and when binding pocket residues are exchanged between two nitrilases that show the same twist but different specificities, their specificities change. It is proposed that helical twist influences the overall size of the binding pocket
-
-
?
3-chloro-4-fluorobenzonitrile + H2O
3-chloro-4-fluorobenzoic acid + NH3
-
-
-
?
3-chlorobenzoylacetonitrile + H2O
?
-
43% of the activity as compared to benzoylacetonitrile
-
-
?
3-hydroxy-3-(2-methoxyphenyl)propanenitrile + H2O
(S)-3-hydroxy-3-(2-methoxyphenyl)propanoic acid + NH3
3-hydroxy-3-(3-methoxyphenyl)propanenitrile + H2O
(S)-3-hydroxy-3-(3-methoxyphenyl)propanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-hydroxy-3-(4-methoxyphenyl)propanenitrile + H2O
(S)-3-hydroxy-3-(4-methoxyphenyl)propanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-hydroxy-3-(4-methylphenyl)propanenitrile + H2O
(S)-3-hydroxy-3-(4-methylphenyl)propanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-hydroxy-3-phenylpropanenitrile + H2O
(S)-3-hydroxy-3-phenylpropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-hydroxy-glutaronitrile + 2 H2O
3-hydroxy-glutaric acid + NH3
-
-
-
?
3-hydroxybenzonitrile + H2O
3-hydroxybenzoate + NH3
-
8.4% activity compared to benzonitrile
-
-
?
3-hydroxyglutaronitrile + 2 H2O
(R)-3-hydroxy-4-cyanobutanoic acid + NH3
-
-
-
-
?
3-hydroxyglutaronitrile + 4 H2O
3-hydroxyglutaric acid + 2 NH3
activity is 1.15% compared to activity with iminodiacetonitrile
-
-
?
3-hydroxyglutaronitrile + H2O
? + NH3
1.15% activity compared to iminodiacetonitrile
-
-
?
3-hydroxypropionitrile + H2O
3-hydroxypropionic acid + NH3
2.57% activity compared to iminodiacetonitrile
-
-
?
3-methoxylbenzoylacetonitrile + H2O
?
-
38% of the activity as compared to benzoylacetonitrile
-
-
?
3-phenylpentanedinitrile + H2O
(3S)-4-cyano-3-phenylbutanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 5.8fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 96% enantiomeric excess by wild-type enzyme and with 96% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-phenylpropionitrile + H2O
3-phenylpropanoate + NH3
activity is 7.24% compared to activity with iminodiacetonitrile
-
-
?
3-phenylpropionitrile + H2O
3-phenylpropionate + NH3
151% of the activity compared to benzonitrile
-
-
?
3-tolunitrile + H2O
3-methylbenzoic acid + NH3
-
5.5% activity compared to benzonitrile
-
-
?
3-trans-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]prop-2-enenitrile + H2O
(2E)-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dien-1-yl]prop-2-enoic acid + NH3
3-[4-(dimethylamino)phenyl]pentanedinitrile + H2O
(3S)-4-cyano-3-[4-(dimethylamino)phenyl]butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 28.0fold higher than activity of wild-type enzyme
-
-
?
3-[4-(methylsulfanyl)phenyl]pentanedinitrile + H2O
(3S)-4-cyano-3-[4-(methylsulfanyl)phenyl]butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 18.0fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 97% enantiomeric excess by wild-type enzyme and with more than 99% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-[4-(propan-2-yl)phenyl]pentanedinitrile + H2O
(3S)-4-cyano-3-[4-(propan-2-yl)phenyl]butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 18.0fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 95% enantiomeric excess by wild-type enzyme and with 98% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
3-[4-(trifluoromethyl)phenyl]pentanedinitrile + H2O
(3S)-4-cyano-3-[4-(trifluoromethyl)phenyl]butanoic acid
the specific activity of mutant enzyme P194A/I201A/F202V is 4.8fold higher than activity of wild-type enzyme The S-enantiomer ist formed with 97% enantiomeric excess by wild-type enzyme and with 96% enantiomeric excess by the mutant enzyme P194A/I201A/F202V
-
-
?
4,5-dimethoxybenzonitrile + 2 H2O
4,5-dimethoxybenzoic acid + NH3
-
-
-
-
?
4-aminobutyronitrile + H2O
4-aminobutyric acid + NH3
251% of the activity compared to benzonitrile
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzoate + NH3
-
29.8% activity compared to benzonitrile
-
-
?
4-chlorobenzoylacetonitrile + H2O
?
-
16% of the activity as compared to benzoylacetonitrile
-
-
?
4-hydroxybenzylcyanide + H2O
4-hydroxybenzoic acid + NH3
low activity
-
-
?
4-methoxy-2-nitrobenzonitrile + 2 H2O
4-methoxy-2-nitrobenzoic acid + NH3
-
-
-
-
?
4-methoxybenzyl cyanide + 2 H2O
4-methoxyphenylacetic acid + NH3
very low activity
-
-
?
4-methoxyphenylacetonitrile + H2O
4-methoxyphenylacetic acid + NH3
-
0.6% activity compared to 3-cyanopyridine
-
-
?
acetamide + H2O
acetate + NH3
-
119% of the activity as compared to acetonitrile
-
-
?
acrylamide + H2O
acrylic acid + NH3
-
101% of the activity as compared to acetonitrile
-
-
?
acrylonitrile + H2O
acrylate + NH3
400% of the activity compared to benzonitrile
-
-
?
alpha,alpha-dimethylmalononitrile + H2O
2-cyano-2-methylpropanoic acid + NH3
-
93% yield
-
-
?
aminoacetonitrile + 2 H2O
aminoacetic acid + NH3
25% of the activity with acrylonitrile
-
-
?
aminoacetonitrile + H2O
?
about 30% activity compared to acrylonitrile
-
-
?
aminoacetonitrile + H2O
aminoacetic acid + NH3
-
9.0% activity compared to 3-cyanopyridine
-
-
?
beta-aminopropionitrile + H2O
beta-aminopropionic acid + NH3
-
8.6% activity compared to 3-cyanopyridine
-
-
?
bis-acrylamide + H2O
?
-
67% of the activity as compared to acetonitrile
-
-
?
butanedinitrile + H2O
? + NH3
104.8% activity compared to iminodiacetonitrile
-
-
?
chloroacetonitrile + H2O
chloroacetic acid + NH3
low activity
-
-
?
chloroxynil + H2O
?
-
3',5-dihalogenated 4-hydroxybenzonitrile compound
-
-
?
cinnamonitrile + 2 H2O
cinnamic acid + NH3
cinnamonitrile is a substrate for mutant enzyme K96R, no activity with wild-type enzyme
-
-
?
cis-1,2-dihydroxy-3-cyanocyclohexa-3,5-diene + H2O
5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid + NH3
-
-
-
-
?
cis-5,6-dihydroxy-cyclohexa-1,3-diene-1-carbonitrile + H2O
(5R,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid + NH3
cyclopropanecarbonitrile + H2O
cyclopropanecarboxylic acid + NH3
-
-
-
?
di-phenylacetonitrile + H2O
? + NH3
72% of the activity compared to benzonitrile
-
-
?
DL-alpha-amino-4-methylthio butyronitrile + H2O
L-methionine + NH3
-
-
-
?
DL-alpha-amino-n-capronitrile + H2O
L-norleucine + NH3
-
-
-
?
DL-alpha-amino-n-valeronitrile + H2O
L-norvaline + NH3
-
-
-
?
glutaronitrile + H2O
? + NH3
319% of the activity compared to benzonitrile
-
-
?
glycinonitrile + H2O
?
-
23.27% activity compared to 3-cyanopyridine
-
-
?
hydroxy(4-methylphenyl)acetonitrile + H2O
2-hydroxy-2-(4-methylphenyl)acetamide
-
-
-
?
hydroxy(phenyl)acetonitrile + H2O
?
-
hydrolysis of alpha-hydroxynitriles is not enantioselective
-
-
?
hydroxy(phenyl)acetonitrile + H2O
hydroxy(phenyl)acetic acid + 2-hydroxy-2-phenylacetamide
isobutyronitrile + H2O
isobutyric acid + NH3
-
4% activity compared to benzonitrile
-
-
?
isovaleronitrile + H2O
isovaleric acid + NH3
-
47.17% activity compared to 3-cyanopyridine
-
-
?
lactonitrile + H2O
lactic acid + NH3
-
4% activity compared to benzonitrile
-
-
?
mandelonitrile + H2O
? + NH3
69% of the activity compared to benzonitrile
-
-
?
N-methyl-propionamide + H2O
?
-
161% of the activity as compared to acetonitrile
-
-
?
pyrazinecarbonitrile + H2O
?
-
32.7% activity compared to 3-cyanopyridine
-
-
?
R(+)-mandelonitrile + H2O
?
316% substrate conversion compared to benzonitrile
-
-
?
thiophen-2-acetonitrile + H2O
thiophen-2-ylacetic acid + NH3
-
56.1% activity compared to benzonitrile
-
-
?
trans-3-pentenenitrile + H2O
trans-3-pentenoic acid + NH3
-
-
-
?
trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile + H2O
trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid + NH3
trichloroacetonitrile + 2 H2O
trichloroacetate + NH3
-
-
-
?
2-(2-chlorophenyl)-2-hydroxyacetamide
-
-
-
?
(2-chlorophenyl)(hydroxy)acetonitrile + H2O
2-(2-chlorophenyl)-2-hydroxyacetamide
-
-
-
?
2-(2-ethylphenyl)-2-hydroxyacetamide
-
-
-
?
(2-ethylphenyl)(hydroxy)acetonitrile + H2O
2-(2-ethylphenyl)-2-hydroxyacetamide
-
-
-
?
?
-
poor substrate
-
-
?
(2S)-1-[(4-methylphenyl)sulfonyl]piperidine-2-carbonitrile + H2O
?
-
poor substrate
-
-
?
(2S)-1-[(4-methylphenyl)sulfonyl]piperidine-2-carbonitrile + H2O
?
-
poor substrate
-
-
?
(2S)-1-[(4-methylphenyl)sulfonyl]piperidine-2-carbonitrile + H2O
?
-
poor substrate
-
-
?
(3E)-2-hydroxypent-3-enamide
-
-
-
?
(3E)-2-hydroxypent-3-enenitrile + H2O
(3E)-2-hydroxypent-3-enamide
-
-
-
?
?
-
poor substrate
-
-
?
(3R)-1-[(4-methylphenyl)sulfonyl]piperidine-3-carbonitrile + H2O
?
-
poor substrate
-
-
?
(3R)-1-[(4-methylphenyl)sulfonyl]piperidine-3-carbonitrile + H2O
?
-
poor substrate
-
-
?
(3R)-1-[(4-methylphenyl)sulfonyl]piperidine-3-carbonitrile + H2O
?
-
poor substrate
-
-
?
?
-
preferred substrate, amides are by-products of the nitrilase-catalyzed reaction
-
-
?
(3R)-3-methyl-1-[(4-methylphenyl)sulfonyl]pyrrolidine + H2O
?
-
preferred substrate, amides are by-products of the nitrilase-catalyzed reaction
-
-
?
(3R)-3-methyl-1-[(4-methylphenyl)sulfonyl]pyrrolidine + H2O
?
-
preferred substrate, amides are by-products of the nitrilase-catalyzed reaction
-
-
?
(3R)-3-methyl-1-[(4-methylphenyl)sulfonyl]pyrrolidine + H2O
?
-
preferred substrate, amides are by-products of the nitrilase-catalyzed reaction
-
-
?
(3S)-3-(2,4-difluorophenyl)-3-hydroxypropanoic acid + NH3
-
91% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(2,4-difluorophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(2,4-difluorophenyl)-3-hydroxypropanoic acid + NH3
-
91% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(3-methoxyphenyl)-3-hydroxypropanoic acid + NH3
-
88% yield, 95% enantiomeric excess
-
-
?
(3S)-3-(3-methoxyphenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(3-methoxyphenyl)-3-hydroxypropanoic acid + NH3
-
88% yield, 95% enantiomeric excess
-
-
?
(3S)-3-(3-nitrophenyl)-3-hydroxypropanoic acid + NH3
-
88% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(3-nitrophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(3-nitrophenyl)-3-hydroxypropanoic acid + NH3
-
88% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(4-bromophenyl)-3-hydroxypropanoic acid + NH3
-
89% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(4-bromophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-bromophenyl)-3-hydroxypropanoic acid + NH3
-
89% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(4-chlorophenyl)-3-hydroxypropanoic acid + NH3
-
92% yield, 99% enantiomeric excess
-
-
?
(3S)-3-(4-chlorophenyl)-3-hydroxypropanenitrile + H2O
(3S)-3-(4-chlorophenyl)-3-hydroxypropanoic acid + NH3
-
92% yield, 99% enantiomeric excess
-
-
?
(E)-2-methylbut-2-enoic acid + NH3
-
-
-
-
?
(E/Z)-2-methylbut-2-enenitrile + H2O
(E)-2-methylbut-2-enoic acid + NH3
-
-
-
-
?
(E/Z)-2-methylbut-2-enenitrile + H2O
(E)-2-methylbut-2-enoic acid + NH3
-
-
-
-
?
(E/Z)-2-methylbut-2-enenitrile + H2O
(E)-2-methylbut-2-enoic acid + NH3
-
-
-
-
?
(E/Z)-2-methylbut-2-enenitrile + H2O
(E)-2-methylbut-2-enoic acid + NH3
-
-
-
-
?
(methylthio)acetic acid + NH3
-
-
-
?
(R)-2-chloromandelic acid + (S)-2-chloromandelic acid + NH3
-
the wild type enzyme yields 76% (R)-2-chloromandelic acid and 9% (S)-2-chloromandelic acid at pH 7.5, as well as 12% (R)-2-chloromandelic acid and no (S)-2-chloromandelic acid at pH 4.5
-
?
(R)-2-chloromandelonitrile + H2O
(R)-2-chloromandelic acid + (S)-2-chloromandelic acid + NH3
-
the wild type enzyme yields 76% (R)-2-chloromandelic acid and 9% (S)-2-chloromandelic acid at pH 7.5, as well as 12% (R)-2-chloromandelic acid and no (S)-2-chloromandelic acid at pH 4.5
-
?
(R)-2-hydroxy-2-phenylacetic acid + NH3
i.e. (R,S)-mandelonitrile, preferred substrate, highly enantioselective reaction
-
-
?
(R,S)-2-hydroxy-2-phenylacetonitrile + 2 H2O
(R)-2-hydroxy-2-phenylacetic acid + NH3
i.e. (R,S)-mandelonitrile, preferred substrate, highly enantioselective reaction
-
-
?
?
-
1% activity compared to benzonitrile
-
-
?
(R,S)-2-phenylpropionitrile + H2O
?
-
1% activity compared to benzonitrile
-
-
?
(S)-2-acetoxy-2-phenylacetic acid + (S)-2-acetoxy-2-phenylacetamide
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
(S)-2-acetoxy-2-phenylacetonitrile + H2O
(S)-2-acetoxy-2-phenylacetic acid + (S)-2-acetoxy-2-phenylacetamide
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
1,4-dicyanobutane + H2O
hexanedioic acid + NH3
-
97% yield
-
-
?
?
-
preferred substrate
-
-
?
1-[(4-methylphenyl)sulfonyl]piperidine-4-carbonitrile + H2O
?
-
preferred substrate
-
-
?
1-[(4-methylphenyl)sulfonyl]piperidine-4-carbonitrile + H2O
?
-
preferred substrate
-
-
?
1-[(4-methylphenyl)sulfonyl]piperidine-4-carbonitrile + H2O
?
-
preferred substrate
-
-
?
(R)-(-)-mandelic acid + (S)-mandelonitrile
-
-
-
-
?
2 (R,S)-mandelonitrile + 2 H2O
(R)-(-)-mandelic acid + (S)-mandelonitrile
-
-
-
-
?
(R)-mandelic acid + NH3 + (S)-mandelonitrile
-
-
-
-
?
2 (R,S)-mandelonitrile + 4 H2O
(R)-mandelic acid + NH3 + (S)-mandelonitrile
-
-
-
-
?
2 (R,S)-mandelonitrile + 4 H2O
(R)-mandelic acid + NH3 + (S)-mandelonitrile
-
-
-
-
?
phenylacetic acid + 2-phenylacetamide + NH3
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
2 2-phenylacetonitrile + 3 H2O
phenylacetic acid + 2-phenylacetamide + NH3
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
2-phenylpropanoic acid + 2-phenylpropanamide + NH3
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
2 2-phenylpropionitrile + 3 H2O
2-phenylpropanoic acid + 2-phenylpropanamide + NH3
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
phenylacetamide + phenylacetate + NH3
-
in the presence of organic solvents, 25-95%, v/v
-
?
2 phenylacetonitrile + 3 H2O
phenylacetamide + phenylacetate + NH3
-
-
not completely converted to acid, 2% of the product is phenylacetamide
?
2-chloro-2-phenylacetic acid + 2-chloro-2-phenylacetamide
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
2-chloro-2-phenylacetonitrile + H2O
2-chloro-2-phenylacetic acid + 2-chloro-2-phenylacetamide
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
?
-
7.48% activity compared to 3-cyanopyridine
-
-
?
2-chloro-4-cyanopyridine + H2O
?
-
75.9% activity compared to 3-cyanopyridine
-
-
?
2-chloromandelonitrile + 2 H2O
2-chloromandelic acid + NH3
-
-
-
?
?
-
7% of the activity as compared to benzoylacetonitrile
-
-
?
2-chloromandelonitrile + H2O
?
-
7% of the activity as compared to benzoylacetonitrile
-
-
?
2-pyridine carboxylic acid + NH3
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
low activity
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
110% of the activity compared to benzonitrile
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
3.27% activity compared to iminodiacetonitrile
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
activity is 3.27% compared to activity with iminodiacetonitrile
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
1.5% activity compared to 3-cyanopyridine
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
pyridine-2-carboxylic acid + NH3
-
14.2% activity compared to benzonitrile
-
-
?
2-furanocarboxylic acid + NH3
-
171% activity compared to benzonitrile
-
-
?
2-furanocarbonnitrile + H2O
2-furanocarboxylic acid + NH3
-
171% activity compared to benzonitrile
-
-
?
2-furanocarbonnitrile + H2O
2-furanocarboxylic acid + NH3
-
171% activity compared to benzonitrile
-
-
?
2-hydroxy-2-phenylpropionic acid + NH3
-
acetophenone cyanohydrin, the substrate is transformed in cells to the corresponding acid (atrolactate) and amide (atrolactamide) at a ratio of about 3.4:1. The (R)-acid and the (S)-amide are formed preferentially from acetophenone cyanohydrin
i.e. atrolactic acid
-
?
2-hydroxy-2-phenylpropionitrile + 2 H2O
2-hydroxy-2-phenylpropionic acid + NH3
-
acetophenone cyanohydrin, the substrate is transformed in cells to the corresponding acid (atrolactate) and amide (atrolactamide) at a ratio of about 3.4:1. The (R)-acid and the (S)-amide are formed preferentially from acetophenone cyanohydrin
i.e. atrolactic acid
-
?
2-hydroxy-2-phenylpropionitrile + 2 H2O
2-hydroxy-2-phenylpropionic acid + NH3
acetophenone cyanohydrin, the substrate is transformed in cells to the corresponding acid (atrolactate) and amide (atrolactamide) at a ratio of about 3.4:1. The (R)-acid and the (S)-amide are formed preferentially from acetophenone cyanohydrin
i.e. atrolactic acid
-
?
2-hydroxy-2-phenylpropionitrile + 2 H2O
2-hydroxy-2-phenylpropionic acid + NH3
acetophenone cyanohydrin, the substrate is transformed in cells to the corresponding acid (atrolactate) and amide (atrolactamide) at a ratio of about 3.4:1. The (R)-acid and the (S)-amide are formed preferentially from acetophenone cyanohydrin
i.e. atrolactic acid
-
?
2-hydroxy-2-phenylpropionitrile + 2 H2O
2-hydroxy-2-phenylpropionic acid + NH3
-
acetophenone cyanohydrin, the substrate is transformed in cells to the corresponding acid (atrolactate) and amide (atrolactamide) at a ratio of about 3.4:1. The (R)-acid and the (S)-amide are formed preferentially from acetophenone cyanohydrin
i.e. atrolactic acid
-
?
2-hydroxy-2-phenylpropionitrile + 2 H2O
2-hydroxy-2-phenylpropionic acid + NH3
-
acetophenone cyanohydrin, the substrate is transformed in cells to the corresponding acid (atrolactate) and amide (atrolactamide) at a ratio of about 3.4:1. The (R)-acid and the (S)-amide are formed preferentially from acetophenone cyanohydrin
i.e. atrolactic acid
-
?
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
substrate contains a quaternary carbon atom in the alpha-position toward the nitrile group
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
substrate contains a quaternary carbon atom in the alpha-position toward the nitrile group
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
substrate contains a quaternary carbon atom in the alpha-position toward the nitrile group
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
substrate contains a quaternary carbon atom in the alpha-position toward the nitrile group
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
substrate contains a quaternary carbon atom in the alpha-position toward the nitrile group
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
substrate contains a quaternary carbon atom in the alpha-position toward the nitrile group
-
-
?
2-methylglutaronitrile + 2 H2O
4-cyanopentanoic acid + NH3
-
-
-
-
?
2-methylglutaronitrile + 2 H2O
4-cyanopentanoic acid + NH3
regioselectivity of Nit1
-
-
?
2-phenylpropionic acid + NH3
-
-
-
?
2-phenylpropionitrile + 2 H2O
2-phenylpropionic acid + NH3
-
-
-
?
2-thiophenacetic acid + NH3
-
strain JM3
-
-
?
2-thiophenacetonitrile + H2O
2-thiophenacetic acid + NH3
-
strain JM3
-
-
?
3,5-dibromo-4-hydroxybenzoic acid + NH3
-
i.e. bromoxynil, is a better substrate than ioxynil, but shows lower reaction rate than chloroxynil and benzonitrile
-
-
?
3,5-dibromo-4-hydroxybenzonitrile + H2O
3,5-dibromo-4-hydroxybenzoic acid + NH3
-
i.e. bromoxynil, is a better substrate than ioxynil, but shows lower reaction rate than chloroxynil and benzonitrile
-
-
?
3,5-dibromo-4-hydroxybenzonitrile + H2O
3,5-dibromo-4-hydroxybenzoic acid + NH3
-
i.e. bromoxynil, is a better substrate than ioxynil, but shows lower reaction rate than chloroxynil and benzonitrile
-
-
?
3,5-dibromo-4-hydroxybenzonitrile + H2O
3,5-dibromo-4-hydroxybenzoic acid + NH3
-
i.e. bromoxynil, is a better substrate than ioxynil, but shows lower reaction rate than chloroxynil and benzonitrile
-
-
?
3,5-dibromo-4-hydroxybenzonitrile + H2O
3,5-dibromo-4-hydroxybenzoic acid + NH3
-
i.e. bromoxynil, is a better substrate than ioxynil, but shows lower reaction rate than chloroxynil and benzonitrile
-
-
?
3,5-dibromo-4-hydroxybenzonitrile + H2O
3,5-dibromo-4-hydroxybenzoic acid + NH3
Rhodococcus sp. NDB 1165
-
i.e. bromoxynil, is a better substrate than ioxynil, but shows lower reaction rate than chloroxynil and benzonitrile
-
-
?
3,5-dichloro-4-hydroxybenzoic acid + NH3
-
i.e. chloroxynil, is a better substrate than bromoxynil and ioxynil, but shows lower reaction rate than benzonitrile
-
-
?
3,5-dichloro-4-hydroxybenzonitrile + H2O
3,5-dichloro-4-hydroxybenzoic acid + NH3
-
i.e. chloroxynil, is a better substrate than bromoxynil and ioxynil, but shows lower reaction rate than benzonitrile
-
-
?
3,5-dichloro-4-hydroxybenzonitrile + H2O
3,5-dichloro-4-hydroxybenzoic acid + NH3
-
i.e. chloroxynil, is a better substrate than bromoxynil and ioxynil, but shows lower reaction rate than benzonitrile
-
-
?
3,5-dichloro-4-hydroxybenzonitrile + H2O
3,5-dichloro-4-hydroxybenzoic acid + NH3
-
i.e. chloroxynil, is a better substrate than bromoxynil and ioxynil, but shows lower reaction rate than benzonitrile
-
-
?
3,5-dichloro-4-hydroxybenzonitrile + H2O
3,5-dichloro-4-hydroxybenzoic acid + NH3
-
i.e. chloroxynil, is a better substrate than bromoxynil and ioxynil, but shows lower reaction rate than benzonitrile
-
-
?
3,5-dichloro-4-hydroxybenzonitrile + H2O
3,5-dichloro-4-hydroxybenzoic acid + NH3
Rhodococcus sp. NDB 1165
-
i.e. chloroxynil, is a better substrate than bromoxynil and ioxynil, but shows lower reaction rate than benzonitrile
-
-
?
3,5-diiodo-4-hydroxybenzoic acid + NH3
-
worst substrate with lowest reaction rate
-
-
?
3,5-diiodo-4-hydroxybenzonitrile + H2O
3,5-diiodo-4-hydroxybenzoic acid + NH3
-
worst substrate with lowest reaction rate
-
-
?
3,5-diiodo-4-hydroxybenzonitrile + H2O
3,5-diiodo-4-hydroxybenzoic acid + NH3
-
worst substrate with lowest reaction rate
-
-
?
3,5-diiodo-4-hydroxybenzonitrile + H2O
3,5-diiodo-4-hydroxybenzoic acid + NH3
-
worst substrate with lowest reaction rate
-
-
?
3,5-diiodo-4-hydroxybenzonitrile + H2O
3,5-diiodo-4-hydroxybenzoic acid + NH3
-
worst substrate with lowest reaction rate
-
-
?
3,5-diiodo-4-hydroxybenzonitrile + H2O
3,5-diiodo-4-hydroxybenzoic acid + NH3
Rhodococcus sp. NDB 1165
-
worst substrate with lowest reaction rate
-
-
?
(S)-3-(2,4-dichlorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-(2,4-dichlorophenyl)-3-hydroxypropanenitrile + H2O
(S)-3-(2,4-dichlorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-(2-chlorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-(2-chlorophenyl)-3-hydroxypropanenitrile + H2O
3-(2-chlorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
(S)-3-(4-chlorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-(4-chlorophenyl)-3-hydroxypropanenitrile + H2O
(S)-3-(4-chlorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
(S)-3-(4-fluorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-(4-fluorophenyl)-3-hydroxypropanenitrile + H2O
(S)-3-(4-fluorophenyl)-3-hydroxypropanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-aminobenzonitrile + H2O
3-aminobenzoic acid + NH3
-
2% activity compared to benzonitrile
-
-
?
3-aminobenzonitrile + H2O
3-aminobenzoic acid + NH3
-
-
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
-
41% activity compared to benzonitrile
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
-
41% activity compared to benzonitrile
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
-
87% activity compared to benzonitrile
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
-
11% activity compared to benzonitrile
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
-
11% activity compared to benzonitrile
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
-
87% activity compared to benzonitrile
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
-
11% activity compared to benzonitrile
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzoic acid + NH3
Pyrococcus sp. MC-FB
-
14% of the activity compared to 2-cyanopyridine
-
-
?
3-pyridinecarboxylic acid + NH3
-
28% activity compared to benzonitrile
-
-
?
3-cyanopyridine + 2 H2O
3-pyridinecarboxylic acid + NH3
-
41% activity compared to benzonitrile
-
-
?
3-cyanopyridine + 2 H2O
nicotinic acid + NH3
-
100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine
-
-
?
3-cyanopyridine + 2 H2O
nicotinic acid + NH3
-
lower activity for the purified enzyme compared to the activity in resting cells
-
?
3-cyanopyridine + 2 H2O
nicotinic acid + NH3
about 50% activity compared to acrylonitrile
-
-
?
3-cyanopyridine + 2 H2O
nicotinic acid + NH3
about 50% activity compared to acrylonitrile
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
low activity
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
102% of the activity as compared to acetonitrile
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
50% of the activity with acrylonitrile
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
50% of the activity with acrylonitrile
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
?
pyridine-3-carboxylic acid + NH3
-
32.4% activity compared to benzonitrile
-
-
?
3-cyanopyridine + 2 H2O
pyridine-3-carboxylic acid + NH3
151% of the activity compared to benzonitrile
-
-
?
3-cyanopyridine + 2 H2O
pyridine-3-carboxylic acid + NH3
Pyrococcus sp. MC-FB
-
30% of the activity compared to 2-cyanopyridine
-
-
?
pyridine 3-carboxylic acid + NH3
-
-
-
?
(S)-3-hydroxy-3-(2-methoxyphenyl)propanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-hydroxy-3-(2-methoxyphenyl)propanenitrile + H2O
(S)-3-hydroxy-3-(2-methoxyphenyl)propanoic acid + NH3
-
nitrilase bll6402 catalyzes the enantioselective hydrolysis of aromatic beta-hydroxy nitriles to give (S)-enriched beta-hydroxy carboxylic acids with recovery of (R)-enriched beta-hydroxy nitriles
-
-
?
3-hydroxy-3-phenylpropionic acid + NH3
-
beta-substituted substrate
-
-
?
3-hydroxy-3-phenylpropionitrile + H2O
3-hydroxy-3-phenylpropionic acid + NH3
Rhodococcus sp. Novo SP361
-
beta-substituted substrate
-
-
?
3-hydroxybenzonitrile + H2O
3-hydroxybenzoic acid + NH3
-
1% activity compared to benzonitrile
-
-
?
3-hydroxybenzonitrile + H2O
3-hydroxybenzoic acid + NH3
-
80% activity compared to benzonitrile
-
-
?
3-hydroxypropanoate + NH3
very low activity
-
-
?
3-hydroxypropionitrile + H2O
3-hydroxypropanoate + NH3
low activity
-
-
?
3-hydroxypropionitrile + H2O
3-hydroxypropanoate + NH3
activity is 2.57% compared to activity with iminodiacetonitrile
-
-
?
3-methylbenzonitrile + H2O
3-methylbenzoic acid + NH3
-
-
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
110% of the activity compared to benzonitrile
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
Pyrococcus sp. MC-FB
-
16% of the activity compared to 2-cyanopyridine
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
-
174.8% activity compared to benzonitrile
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
-
841.7% activity compared to benzonitrile
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
Rhodococcus sp. NCIMB 11215
-
174.8% activity compared to benzonitrile
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
Rhodococcus sp. NCIMB 11215
-
841.7% activity compared to benzonitrile
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
Rhodococcus sp. NCIMB 11216
-
174.8% activity compared to benzonitrile
-
-
?
3-nitrobenzonitrile + H2O
3-nitrobenzoic acid + NH3
Rhodococcus sp. NCIMB 11216
-
841.7% activity compared to benzonitrile
-
-
?
3-phenylpropionic acid + NH3
the enzyme has 270times more activity with 3-phenylpropionitrile than that observed with benzonitrile
-
-
?
3-phenylpropionitrile + H2O
3-phenylpropionic acid + NH3
7.24% activity compared to iminodiacetonitrile
-
-
?
3-phenylpropionitrile + H2O
3-phenylpropionic acid + NH3
7.24% activity compared to iminodiacetonitrile
-
-
?
3-phenylpropionic acid + NH3
1650% substrate conversion compared to benzonitrile
-
-
?
3-phenylproprionitrile + H2O
3-phenylpropionic acid + NH3
1650% substrate conversion compared to benzonitrile
-
-
?
3-methylbenzoate + NH3
-
33% activity compared to benzonitrile
-
-
?
3-tolunitrile + H2O
3-methylbenzoate + NH3
-
9% activity compared to benzonitrile
-
-
?
(2E)-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dien-1-yl]prop-2-enoic acid + NH3
-
-
-
-
?
3-trans-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]prop-2-enenitrile + H2O
(2E)-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dien-1-yl]prop-2-enoic acid + NH3
-
-
-
-
?
3-trans-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]prop-2-enenitrile + H2O
(2E)-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dien-1-yl]prop-2-enoic acid + NH3
-
-
-
-
?
4-aminobenzoic acid + NH3
-
7% activity compared to benzonitrile
-
-
?
4-aminobenzonitrile + H2O
4-aminobenzoic acid + NH3
-
-
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzoic acid + NH3
-
40% activity compared to benzonitrile
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzoic acid + NH3
-
19% activity compared to benzonitrile
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzoic acid + NH3
-
19% activity compared to benzonitrile
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzoic acid + NH3
-
40% activity compared to benzonitrile
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzoic acid + NH3
-
19% activity compared to benzonitrile
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzoic acid + NH3
Pyrococcus sp. MC-FB
-
11% of the activity compared to 2-cyanopyridine
-
-
?
?
329% substrate conversion compared to benzonitrile
-
-
?
4-chlorobenzonitrile + H2O
?
329% substrate conversion compared to benzonitrile
-
-
?
4-chlorobutyrate + NH3
activity is 11.1% compared to activity with iminodiacetonitrile
-
-
?
4-chlorobutyronitrile + H2O
4-chlorobutyric acid + NH3
11.1% activity compared to iminodiacetonitrile
-
-
?
4-pyridinecarboxylic acid + NH3
-
best substrate
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
1138% substrate conversion compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
1138% substrate conversion compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
best substrate
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
best substrate
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
130% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
best substrate showing 217% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
130% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
best substrate showing 217% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
130% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
130% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
4-pyridinecarboxylic acid + NH3
-
best substrate showing 217% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
0.07% activity compared to 3-cyanopyridine
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
2.48% activity compared to iminodiacetonitrile
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
79.5% activity compared to 3-cyanopyridine
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
-
-
?
pyridine-4-carboxylic acid + NH3
-
410.7% activity compared to benzonitrile
-
-
?
4-cyanopyridine + 2 H2O
pyridine-4-carboxylic acid + NH3
225% of the activity compared to benzonitrile
-
-
?
4-cyanopyridine + H2O
pyridine 4-carboxylic acid + NH3
410.7% activity compared to benzonitrile
-
-
?
4-cyanopyridine + H2O
pyridine 4-carboxylic acid + NH3
410.7% activity compared to benzonitrile
-
-
?
4-cyanopyridine + H2O
pyridine 4-carboxylic acid + NH3
activity is 2.48% compared to activity with iminodiacetonitrile
-
-
?
4-hydroxybenzonitrile + H2O
4-hydroxybenzoic acid + NH3
-
3% activity compared to benzonitrile
-
-
?
4-hydroxybenzonitrile + H2O
4-hydroxybenzoic acid + NH3
-
very low activity
-
?
4-hydroxyphenylacetonitrile + H2O
4-hydroxyphenylacetic acid + NH3
-
substrate for isozyme complex NIT4A/B2
-
-
?
4-nitrobenzonitrile + H2O
4-nitrobenzoic acid + NH3
151% of the activity compared to benzonitrile
-
-
?
4-nitrobenzonitrile + H2O
4-nitrobenzoic acid + NH3
Pyrococcus sp. MC-FB
-
11% of the activity compared to 2-cyanopyridine
-
-
?
4-phenylbutyric acid + NH3
-
-
-
?
4-methylbenzoate + NH3
-
16% activity compared to benzonitrile
-
-
?
4-tolunitrile + H2O
4-methylbenzoate + NH3
-
5% activity compared to benzonitrile
-
-
?
4-methylbenzoic acid + NH3
-
125% activity compared to benzonitrile
-
-
?
4-tolunitrile + H2O
4-methylbenzoic acid + NH3
-
3.4% activity compared to benzonitrile
-
-
?
6-heptenoic acid + NH3
the enzyme shows a preference for several aliphatic alkenenitriles with peak activity against 6-heptenenitrile. Helical twist and substrate size correlate and when binding pocket residues are exchanged between two nitrilases that show the same twist but different specificities, their specificities change. It is proposed that helical twist influences the overall size of the binding pocket
-
-
?
6-heptenenitrile + H2O
6-heptenoic acid + NH3
-
substrate for isozyme NIT4A/B2
-
-
?
acetate + NH3
-
2.31% activity compared to 3-cyanopyridine
-
-
?
acetonitrile + H2O
acetate + NH3
135% of the activity compared to benzonitrile
-
-
?
acetonitrile + H2O
acetate + NH3
135% of the activity compared to benzonitrile
-
-
?
acetonitrile + H2O
acetate + NH3
-
2.31% activity compared to 3-cyanopyridine
-
-
?
acetic acid + NH3
-
12% of the activity as compared to benzoylacetonitrile
-
-
?
acetonitrile + H2O
acetic acid + NH3
-
12% of the activity as compared to benzoylacetonitrile
-
-
?
acetonitrile + H2O
acetic acid + NH3
-
1.0% activity compared to 3-cyanopyridine
-
-
?
acrylonitrile + 2 H2O
acrylic acid + NH3
-
preferred substrate, acrylonitrile is also a good inducer of enzyme production
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
35% activity compared to benzonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
43.96% activity compared to 3-cyanopyridine
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
43.96% activity compared to 3-cyanopyridine
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
6.6% activity compared to benzonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
6.6% activity compared to benzonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
6.6% activity compared to benzonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
107% of the activity as compared to acetonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
107% of the activity as compared to acetonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
17.2% activity compared to 3-cyanopyridine
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
17.2% activity compared to 3-cyanopyridine
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
best substrate, no formation of amide
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate
-
?
acrylonitrile + H2O
acrylic acid + NH3
100% activity, the optimum substrate concentration is 225 mM acrylonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
22.4% activity compared to benzonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
22.4% activity compared to benzonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
22.4% activity compared to benzonitrile
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
100% activity, the optimum substrate concentration is 225 mM acrylonitrile
-
-
?
adiponitrile + H2O
adipic acid + NH3
preferred substrate, specific for aliphatic nitriles
-
?
adiponitrile + H2O
adipic acid + NH3
no formation of amide
-
?
adiponitrile + H2O
adipic acid + NH3
-
106.29% activity compared to 3-cyanopyridine
-
-
?
adiponitrile + H2O
adipic acid + NH3
-
106.29% activity compared to 3-cyanopyridine
-
-
?
adiponitrile + H2O
adipic acid + NH3
activity is 27.1% compared to activity with iminodiacetonitrile
-
-
?
adiponitrile + H2O
adipic acid + NH3
Pyrococcus sp. MC-FB
-
3% of the activity compared to 2-cyanopyridine
-
-
?
prop-2-enoic acid + NH3
-
-
-
?
allyl cyanide + H2O
prop-2-enoic acid + NH3
high activity
-
-
?
benzoic acid + NH3
-
257% of the activity as compared to acetonitrile
-
-
?
benzamide + H2O
benzoic acid + NH3
-
257% of the activity as compared to acetonitrile
-
-
?
benzamide + H2O
benzoic acid + NH3
-
catalysed by a highly purified nitrilase, but very low activity
-
?
benzonitrile + 2 H2O
benzoate + NH3
preferred substrate
-
-
?
benzonitrile + 2 H2O
benzoate + NH3
-
94% of the activity as compared to acetonitrile
-
-
?
benzonitrile + 2 H2O
benzoate + NH3
-
94% of the activity as compared to acetonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
100% substrate conversion
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
100% activity
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
100% substrate conversion
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
preferred substrate, when the cells are induced by benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
preferred substrate, when the cells are induced by benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
100% activity
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
42.14% activity compared to 3-cyanopyridine
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
100% activity
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
100% activity
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
only traces of activity
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
best substrate with highest reaction rate
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
best substrate with highest reaction rate
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
specific for nitrile groups directly attached to the benzene ring
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
specific for nitrile groups directly attached to the benzene ring
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
high activity in strain NCIB11216 and strain NCIB11215
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
70.4% activity compared to 3-cyanopyridine
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
70.4% activity compared to 3-cyanopyridine
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
Pyrococcus sp. MC-FB
-
7% of the activity compared to 2-cyanopyridine
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
-
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
best substrate with highest reaction rate
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
100% activity
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
best substrate with highest reaction rate
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
best substrate with highest reaction rate
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
Rhodococcus sp. NDB 1165
-
best substrate with highest reaction rate
-
-
?
?
-
preferred substrate
-
-
?
benzyl (3R)-3-methylpyrrolidine-1-carboxylate + H2O
?
-
preferred substrate
-
-
?
?
-
preferred substrate
-
-
?
benzyl [(1R,3R)-3-cyanocyclohexyl]carbamate + H2O
?
-
preferred substrate
-
-
?
?
-
preferred substrate
-
-
?
benzyl [(1S,3R)-3-cyanocyclohexyl]carbamate + H2O
?
-
preferred substrate
-
-
?
3,5-dibromo-4-hydroxybenzoic acid + NH3
-
highly specific
-
?
bromoxynil + H2O
3,5-dibromo-4-hydroxybenzoic acid + NH3
-
highly specific
-
?
?
-
enzyme utilizes the herbicide substrate as nitrogen source
-
-
?
bromoxynil + H2O
?
-
3',5-dihalogenated 4-hydroxybenzonitrile compound, high activity, substrate is a herbicide
-
-
?
bromoxynil + H2O
?
-
strain NCIB11215, very low activity, 3',5-dihalogenated 4-hydroxybenzonitrile compound
-
-
?
butyrate + NH3
425% of the activity compared to benzonitrile
-
-
?
butyronitrile + H2O
butyrate + NH3
425% of the activity compared to benzonitrile
-
-
?
butyronitrile + H2O
butyrate + NH3
-
preference of the enzyme for phenylglycinonitrile
-
-
?
butyric acid + NH3
-
17.6% activity compared to benzonitrile
-
-
?
butyronitrile + H2O
butyric acid + NH3
-
17.6% activity compared to benzonitrile
-
-
?
butyronitrile + H2O
butyric acid + NH3
-
20% activity compared to benzonitrile
-
-
?
cinnamic acid + NH3
7.87% activity compared to iminodiacetonitrile
-
-
?
cinnamonitrile + H2O
cinnamic acid + NH3
activity is 7.87% compared to activity with iminodiacetonitrile
-
-
?
(5R,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid + NH3
-
-
-
-
?
cis-5,6-dihydroxy-cyclohexa-1,3-diene-1-carbonitrile + H2O
(5R,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid + NH3
-
-
-
-
?
crotonic acid + NH3
80.3% activity compared to benzonitrile
-
-
?
crotonitrile + H2O
crotonic acid + NH3
80.3% activity compared to benzonitrile
-
-
?
crotononitrile + H2O
NH3 + (z)-but-2-en-1-ol
-
low activity
-
?
glutaronitrile + H2O
glutaric acid + NH3
activity towards glutaronitrile is 8.5% of that towards succinonitrile
-
-
?
glycolonitrile + 2 H2O
glycolic acid + NH3
40% of the activity with acrylonitrile
-
-
?
?
-
18% of the activity as compared to benzoylacetonitrile
-
-
?
glycolonitrile + H2O
?
about 40% activity compared to acrylonitrile
-
-
?
hydrocinnamonitrile + H2O
hydrocinnamic acid + NH3
-
the enzyme has 431times more activity with hydrocinnamonitrile than that observed with benzonitrile
-
-
?
hydrocinnamonitrile + H2O
hydrocinnamic acid + NH3
-
the enzyme has 431times more activity with hydrocinnamonitrile than that observed with benzonitrile
-
-
?
2-hydroxy-2-(3-phenoxyphenyl)acetamide
-
-
-
?
hydroxy(3-phenoxyphenyl)acetonitrile + H2O
2-hydroxy-2-(3-phenoxyphenyl)acetamide
-
-
-
?
hydroxy(phenyl)acetic acid + 2-hydroxy-2-phenylacetamide
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
hydroxy(phenyl)acetonitrile + H2O
hydroxy(phenyl)acetic acid + 2-hydroxy-2-phenylacetamide
-
the ratio of the nitrilase and nitrile hydratase activities of the enzyme is profoundly influenced by the electronic and steric properties of the reactant
-
-
?
iminodiacetic acid + 2 NH3
high activity
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
-
high activity
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
-
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
-
58.07% activity compared to 3-cyanopyridine
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
-
58.07% activity compared to 3-cyanopyridine
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
100% activity
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
100% activity
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
-
27% activity compared to 3-cyanopyridine
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
-
27% activity compared to 3-cyanopyridine
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
about 25% activity compared to acrylonitrile
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
20% of the activity with acrylonitrile
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
about 25% activity compared to acrylonitrile
-
-
?
iminodiacetonitrile + 2 H2O
iminodiacetic acid + 2 NH3
20% of the activity with acrylonitrile
-
-
?
iminodiacetonitrile + H2O
iminodiacetic acid + NH3
-
-
-
-
?
indol-3-acetonitrile + H2O
indol-3-acetic acid + NH3
-
-
-
?
indole-3-acetonitrile + H2O
indol-3-acetic acid + NH3
-
substrate for isozyme complex NIT4A/B2
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetic acid + NH3
low activity
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetic acid + NH3
-
isozymes are involved in indole-3-acetic acid production
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetic acid + NH3
poor substrate
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetic acid + NH3
-
the reaction is catalyzed by the branch 1 of the nitrilase superfamily
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetic acid + NH3
-
the reaction is catalyzed by the branch 1 of the nitrilase superfamily
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetic acid + NH3
-
preferred substrate of NIT2
-
-
?
?
-
3',5-dihalogenated 4-hydroxybenzonitrile compound
-
-
?
ioxynil + H2O
?
-
3',5-dihalogenated 4-hydroxybenzonitrile compound, strain NCIB11215
-
-
?
malonic acid + NH3
78.9% activity compared to iminodiacetonitrile
-
-
?
malononitrile + H2O
malonic acid + NH3
activity is 78.9% compared to activity with iminodiacetonitrile
-
-
?
malononitrile + H2O
malonic acid + NH3
78.9% activity compared to iminodiacetonitrile
-
-
?
malononitrile + H2O
malonic acid + NH3
-
44% of the activity as compared to acetonitrile
-
-
?
malononitrile + H2O
malonic acid + NH3
-
44% of the activity as compared to acetonitrile
-
-
?
mandelonitrile + 2 H2O
(R)-mandelic acid + NH3
Halomonas nitrilica
-
-
95% enantiomeric excess for (R)-isoform
-
?
mandelonitrile + 2 H2O
(R)-mandelic acid + NH3
-
stereoselective nitrilase
-
-
?
mandelic acid + NH3
-
best substrate of strain ATCC 8750, low activity in strain JM3
-
-
?
mandelonitrile + 2 H2O
mandelic acid + NH3
-
best substrate of strain ATCC 8750, low activity in strain JM3
-
-
?
mandelonitrile + 2 H2O
mandelic acid + NH3
-
high activity
-
-
?
mandelonitrile + 2 H2O
mandelic acid + NH3
2.38% activity compared to iminodiacetonitrile
-
-
?
mandelonitrile + 2 H2O
mandelic acid + NH3
2.38% activity compared to iminodiacetonitrile
-
-
?
?
-
32% of the activity as compared to benzoylacetonitrile
-
-
?
mandelonitrile + H2O
?
-
32% of the activity as compared to benzoylacetonitrile
-
-
?
mandelic acid + NH3
activity is 2.38% compared to activity with iminodiacetonitrile
-
-
?
methylacrylic acid + NH3
-
14% activity compared to benzonitrile
-
-
?
methacrylonitrile + H2O
methylacrylic acid + NH3
-
14% activity compared to benzonitrile
-
-
?
methacrylonitrile + H2O
methylacrylic acid + NH3
-
low activity
-
?
methacrylonitrile + H2O
methylacrylic acid + NH3
-
very low activity in resting cells and for the purified enzyme only after recovered activity by dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate
-
?
?
-
poor substrate
-
-
?
N-[(1R,3R)-3-cyanocyclopentyl]-4-methylbenzenesulfonamide + H2O
?
-
poor substrate
-
-
?
?
-
preferred substrate
-
-
?
N-[(1S,3R)-3-cyanocyclohexyl]-4-methylbenzenesulfonamide + H2O
?
-
preferred substrate
-
-
?
?
-
preferred substrate
-
-
?
N-[(1S,3R)-3-cyanocyclopentyl]-4-methylbenzenesulfonamide + H2O
?
-
preferred substrate
-
-
?
phenylacetate + NH3
-
-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
-
-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
-
21.59% activity compared to 3-cyanopyridine
-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
-
in aqueous solution
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
-
low activity
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
-
-
-
?
phenylacetic acid + NH3
low activity
-
-
?
phenylacetonitrile + 2 H2O
phenylacetic acid + NH3
low activity with the native enzyme, poor activity with the recombinant enzyme
-
-
?
phenylacetonitrile + 2 H2O
phenylacetic acid + NH3
low activity with the native enzyme, poor activity with the recombinant enzyme
-
-
?
?
-
160% of the activity as compared to benzoylacetonitrile
-
-
?
phenylacetonitrile + H2O
?
-
160% of the activity as compared to benzoylacetonitrile
-
-
?
phenylbutyric acid + NH3
-
-
-
?
phenylbutyronitrile + H2O
phenylbutyric acid + NH3
high activity
-
-
?
phenylglycinonitrile + H2O
phenylglycine + NH3
-
preference of the enzyme for phenylglycinonitrile and butyronitrile as substrates
-
-
?
phenylglycinonitrile + H2O
phenylglycine + NH3
-
low actiity
-
?
phenylpropionitrile + 2 H2O
phenylpropionic acid + NH3
best substrate
-
-
?
propionate + NH3
416% of the activity compared to benzonitrile
-
-
?
propionitrile + H2O
propionate + NH3
416% of the activity compared to benzonitrile
-
-
?
propionitrile + H2O
propionic acid + NH3
low activity
-
-
?
propionitrile + H2O
propionic acid + NH3
-
6.9% activity compared to benzonitrile
-
-
?
propionitrile + H2O
propionic acid + NH3
-
6.9% activity compared to benzonitrile
-
-
?
propionitrile + H2O
propionic acid + NH3
-
18% activity compared to benzonitrile
-
-
?
?
-
23% of the activity as compared to acetonitrile
-
-
?
pyridine + H2O
?
-
23% of the activity as compared to acetonitrile
-
-
?
succinate + NH3
activity is 104.8% compared to activity with iminodiacetonitrile
-
-
?
succinonitrile + H2O
succinic acid + NH3
-
64.22% activity compared to 3-cyanopyridine
-
-
?
succinonitrile + H2O
succinic acid + NH3
-
21.74% activity compared to 3-cyanopyridine
-
-
?
succinonitrile + H2O
succinic acid + NH3
Pyrococcus sp. MC-FB
-
18% of the activity compared to 2-cyanopyridine
-
-
?
succinonitrile + H2O
succinic acid + NH3
-
-
-
?
trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid + NH3
-
-
-
-
?
trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile + H2O
trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid + NH3
-
-
-
-
?
valeric acid + NH3
-
19.6% activity compared to benzonitrile
-
-
?
valeronitrile + H2O
valeric acid + NH3
-
19.6% activity compared to benzonitrile
-
-
?
valeronitrile + H2O
valeric acid + NH3
-
45.07% activity compared to 3-cyanopyridine
-
-
?
valeronitrile + H2O
valeric acid + NH3
-
26% activity compared to benzonitrile
-
-
?
valeronitrile + H2O
valeric acid + NH3
4.9% activity compared to iminodiacetonitrile
-
-
?
valeronitrile + H2O
valeric acid + NH3
activity is 4.9% compared to activity with iminodiacetonitrile
-
-
?
additional information
?
-
substrate specificity of immobilized and free Escherichia coli cells recombinantly expressing the Acidovorax facilis nitrilase, overview. No activity with mandelonitrile, o-methoxyphenylacetonitrile, m-methoxyphenylacetonitrile, p-methoxyphenylacetonitrile, and phenylacetonitrile
-
-
?
additional information
?
-
-
substrate specificity of immobilized and free Escherichia coli cells recombinantly expressing the Acidovorax facilis nitrilase, overview. No activity with mandelonitrile, o-methoxyphenylacetonitrile, m-methoxyphenylacetonitrile, p-methoxyphenylacetonitrile, and phenylacetonitrile
-
-
?
additional information
?
-
the enzyme does not hydrolyze benzamide
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze benzamide
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
the enzyme does not hydrolyze benzamide
-
-
?
additional information
?
-
-
the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
-
-
?
additional information
?
-
-
the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. No activity with any inducer on malononitrile
-
-
?
additional information
?
-
-
the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
-
-
?
additional information
?
-
-
the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview
-
-
?
additional information
?
-
-
the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. No activity with any inducer on malononitrile
-
-
?
additional information
?
-
substrate specificity, overview. No activity with diaminomaleonitrile, 2,3-dicyanopyrazine, 4-hydroxybenzonitrile, 2-cyano-3-phenylpropionitrile, 4-aminophthalonitrile, and 4-nitrophthalonitrile
-
-
?
additional information
?
-
substrate specificity, overview. No activity with diaminomaleonitrile, 2,3-dicyanopyrazine, 4-hydroxybenzonitrile, 2-cyano-3-phenylpropionitrile, 4-aminophthalonitrile, and 4-nitrophthalonitrile
-
-
?
additional information
?
-
broad substrate specificity, overview
-
-
?
additional information
?
-
broad substrate specificity, overview
-
-
?
additional information
?
-
broad substrate specificity, overview
-
-
?
additional information
?
-
-
broad substrate specificity, overview
-
-
?
additional information
?
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
-
-
?
additional information
?
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
-
-
?
additional information
?
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
-
-
?
additional information
?
-
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
-
-
?
additional information
?
-
-
does not convert cis-5,6-dihydroxy-cyclohexa-1,3-diene-1-carbonitrile
-
-
?
additional information
?
-
no activity with: benzonitrile, (R,S)-mandelonitrile and
-
-
-
additional information
?
-
no activity with: benzonitrile, (R,S)-mandelonitrile and
-
-
-
additional information
?
-
-
the enzyme shows a strong diastereopreference for cis- versus trans-gamma-amino nitriles
-
-
?
additional information
?
-
substrate specificity and chemoselectivity of native and recombinant enzymes, no activity of both with 2-chlorobenzonitrile, 2-phenylpropionitrile, overview
-
-
?
additional information
?
-
-
the enzyme shows a strong diastereopreference for cis- versus trans-gamma-amino nitriles
-
-
?
additional information
?
-
substrate specificity and chemoselectivity of native and recombinant enzymes, no activity of both with 2-chlorobenzonitrile, 2-phenylpropionitrile, overview
-
-
?
additional information
?
-
-
substrate specificity and chemoselectivity of native and recombinant enzymes, no activity of both with 2-chlorobenzonitrile, 2-phenylpropionitrile, overview
-
-
?
additional information
?
-
-
nitrilase bll6402 effectively hydrolyzes alpha,omega-dinitriles to omega-cyanocarboxylic acids, and the selectivity is independent of the substrate chain length
-
-
?
additional information
?
-
-
nitrilase bll6402 effectively hydrolyzes alpha,omega-dinitriles to omega-cyanocarboxylic acids, and the selectivity is independent of the substrate chain length
-
-
?
additional information
?
-
-
substrate specificity, overview. No activity with phenylacetonitrile, 4-methoxy-phenylacetonitrile, mandelonitrile, 2-amino propionitrile, and butyronitrile
-
-
?
additional information
?
-
Cereibacter sphaeroides LHS-305
-
substrate specificity, overview. No activity with phenylacetonitrile, 4-methoxy-phenylacetonitrile, mandelonitrile, 2-amino propionitrile, and butyronitrile
-
-
?
additional information
?
-
-
no activity with mandelonitrile and geranyl nitrile
-
-
?
additional information
?
-
no activity with fumaronitrile, succinonitrile
-
-
-
additional information
?
-
-
no activity with fumaronitrile, succinonitrile
-
-
-
additional information
?
-
no activity with fumaronitrile, succinonitrile
-
-
-
additional information
?
-
-
no activity with fumaronitrile, succinonitrile
-
-
-
additional information
?
-
-
no activity with mandelonitrile and geranyl nitrile
-
-
?
additional information
?
-
-
does not hydrolyze 3,4-pyridinedicarbonitrile and 2,3-pyrazinedicarbonitrile
-
-
?
additional information
?
-
-
the enzyme is highly chemoselective, producing less than 2% amides as by-products
-
-
?
additional information
?
-
-
the enzyme shows a strong diastereopreference for cis- versus trans-gamma-amino nitriles
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
-
the enzyme is highly chemoselective, producing less than 2% amides as by-products
-
-
?
additional information
?
-
-
the enzyme shows a strong diastereopreference for cis- versus trans-gamma-amino nitriles
-
-
?
additional information
?
-
-
the enzyme is highly chemoselective, producing less than 2% amides as by-products
-
-
?
additional information
?
-
no activity with 2-cyanopyridine
-
-
?
additional information
?
-
-
no activity detected with: benzonitrile, 3-cyanopyridine, 2,3-dicyanopyrazine
-
-
-
additional information
?
-
-
no activity detected with: benzonitrile, 3-cyanopyridine, 2,3-dicyanopyrazine
-
-
-
additional information
?
-
-
stricter substrate specificity compared to nitrilases from other species, strong preference for 4-hydroxybenzonitriles
-
-
?
additional information
?
-
-
the enzyme does not hydrolyzed dichlobenil (2,6-dichlorobenzonitrile)
-
-
?
additional information
?
-
-
the enzyme does not hydrolyzed dichlobenil (2,6-dichlorobenzonitrile)
-
-
?
additional information
?
-
no activity with benzonitrile, phenylacetonitrile, 3-cyanopyridine, glycolonitrile, and dodecanenitrile
-
-
?
additional information
?
-
-
no activity with benzonitrile, phenylacetonitrile, 3-cyanopyridine, glycolonitrile, and dodecanenitrile
-
-
?
additional information
?
-
the suitable substrates for the purified nitrilase were short-chain aliphatic dinitriles
-
-
-
additional information
?
-
no activity with benzonitrile, phenylacetonitrile, 3-cyanopyridine, glycolonitrile, and dodecanenitrile
-
-
?
additional information
?
-
the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
-
-
?
additional information
?
-
-
the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
-
-
?
additional information
?
-
the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
-
-
?
additional information
?
-
-
the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
-
-
?
additional information
?
-
-
no activity with isopentanenitrile, adiponitrile, 2-chloro-3-cyanopyridine, and mandelonitrile
-
-
?
additional information
?
-
-
no activity with isopentanenitrile, adiponitrile, 2-chloro-3-cyanopyridine, and mandelonitrile
-
-
?
additional information
?
-
-
substrate specificity, enzyme catalyzes a range of 2-pyridones with rates of 1-118% relative to ricinine, overview
-
-
?
additional information
?
-
Pyrococcus sp. MC-FB
-
the enzyme preferentially hydrolyzes aromatic nitriles. No activity with: cyclohexanecarbonitrile, malononitrile, butyronitrile, (R,S)-mandelonitrile, 2-phenylglycinonitrile, 2-nitrobenzonitrile
-
-
-
additional information
?
-
-
the enzyme does not hydrolyzed dichlobenil (2,6-dichlorobenzonitrile)
-
-
?
additional information
?
-
-
the wild type enzyme containing Arg129 is active only for meta- and para-substituted benzonitriles with a methyl or amino group, but shows no activity for ortho-substituted benzonitriles
-
-
?
additional information
?
-
-
the wild type enzyme containing Arg129 is active only for meta- and para-substituted benzonitriles with a methyl or amino group, but shows no activity for ortho-substituted benzonitriles
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
-
the enzyme does not hydrolyzed dichlobenil (2,6-dichlorobenzonitrile)
-
-
?
additional information
?
-
-
substrate specificity profile, enzyme also catalyzes the synthesis of a range of alpha-hydroxy carboxylic acids or amides from aldehydes in presence of cyanide
-
-
?
additional information
?
-
-
the enzyme does not hydrolyzed dichlobenil (2,6-dichlorobenzonitrile)
-
-
?
additional information
?
-
-
the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
-
-
?
additional information
?
-
Rhodococcus sp. NDB 1165
-
the enzyme does not hydrolyzed dichlobenil (2,6-dichlorobenzonitrile)
-
-
?
additional information
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Rhodococcus sp. Novo SP361
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substrate specificity profile, enzyme also catalyzes the synthesis of a range of alpha-hydroxy carboxylic acids or amides from aldehydes in presence of cyanide
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recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile
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the enzyme catalyzes the reactions of nitrilase, EC 3.5.5.1, and arylacetonitrilase, EC 3.5.5.5, substrate specificity and enantioselectivity, overview. Steric hindrance with amino acid residue Tyr54 impairs the binding or conversion of sterically demanding substrates, homology modelling
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Nit1 substrate specificity, overview. Significant activities are observed with the aromatic substrates 3-phenylpropionitrile and cinnamonitrile. To a minor degree, the heterocyclic 3-cyanopyridine is also accepted as substrate. In contrast to aliphatic dinitriles, the aliphatic mononitriles acetonitrile, propionitrile, and butyronitrile are almost not converted by Nit1, no activity on malononitrile
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no activity with mandelonitrile, arylacetonitrile and benzonitrile. No activity with acetamide and benzamide
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