3.5.5.1: nitrilase
This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.
Word Map on EC 3.5.5.1
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3.5.5.1
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enantioselectivity
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amidase
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hydratase
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rhodococcus
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biocatalyst
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synthesis
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rhodochrous
-
benzonitrile
-
mandelonitrile
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indole-3-acetic
-
alcaligenes
-
indole-3-acetonitrile
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3-cyanopyridine
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dinitriles
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phenylacetonitrile
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acrylonitrile
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bromoxynil
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r-mandelic
-
nhase
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acidovorax
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ozaenae
-
facilis
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industry
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iminodiacetic
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gibberella
-
analysis
- 3.5.5.1
-
enantioselectivity
- amidase
-
hydratase
- rhodococcus
-
biocatalyst
- synthesis
- rhodochrous
- benzonitrile
- mandelonitrile
-
indole-3-acetic
- alcaligenes
- indole-3-acetonitrile
- 3-cyanopyridine
-
dinitriles
- phenylacetonitrile
- acrylonitrile
- bromoxynil
-
r-mandelic
- nhase
-
acidovorax
- ozaenae
- facilis
- industry
-
iminodiacetic
- gibberella
- analysis
Reaction
Synonyms
3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122
ECTree
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Specific Activity
Specific Activity on EC 3.5.5.1 - nitrilase
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0.1
0.14
mutant enzyme A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 7.5 and 30°C
0.24
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C
0.28
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C
0.4
-
enzyme in cells, substrate 2-methyl-2-phenylpropionitrile in Na/K-phosphate buffer, pH 7.0, 30°C
0.52
mutant enzyme A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 4.5 and 30°C
0.6
-
mutant enzyme N164A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
0.62
wild type enzyme, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 7.5 and 30°C
0.64
recombinant enzyme coexpressed with GroEL/ES, pH 8.0, 30°C
1.9
enzyme in cells, substrate 2-methyl-2-phenylpropionitrile in Na/K-phosphate buffer, pH 7.0, 30°C
10
-
mutant enzyme S188A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
10.4
-
mutant enzyme I104A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
10.7
11.6
14.1
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enzyme in cells, substrate benzonitrile in Na/K-phosphate buffer, pH 7.0, 30°C
14.5
-
mutant enzyme R130A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
15.3
-
for benzonitrile as substrate, for benzamide as substrate very low, but significant specific acitivity
17.7
-
mutant enzyme R130A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
19
substrate mandelonitrile, purified recombinant enzyme, pH 8.0, 40°C
22.4
25.6
purified enzyme, using mandelonitrile as substrate, at 30°C and pH 8.0
26
44
enzyme activity in immobilized cells, pH and temperature not specified in the publication
5.1
-
mutant enzyme V49A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
5.2
-
mutant enzyme M114A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
7.5
-
mutant enzyme T115A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
8
-
mutant enzyme S188A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
8.2
-
mutant enzyme T115A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
8.8
9
-
mutant enzyme Q116A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
9.6
-
mutant enzyme G103A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
9.7
-
mutant enzyme N164A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
9.9
additional information
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after 20.3fold purification, the specific activity is 144 units/mg, while the crude enzyme exhibits a specific activity of 7.1 units/mg at pH 8.0 and 45°C
the specific activity is less than 0.1 micromol/min/mg, wild type enzyme, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 4.5 and 30°C
0.1
-
enzyme in cells, substrate benzonitrile in Na/K-phosphate buffer, pH 7.0, 30°C
10.7
-
wild type enzyme, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
11.6
-
mutant enzymeV49A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
22.4
-
mutant enzyme Y142A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
26
-
mutant enzyme Y142A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
7
-
wild type enzyme, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
-
mutant enzyme G103A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
8.8
-
mutant enzyme I104A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
-
mutant enzyme M114A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
9.9
-
mutant enzyme Q116A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C