3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Reaction

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.5 In nitriles

                3.5.5.1 nitrilase

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Results	in table
22	Activating Compound
841	AA Sequence
74	Application
7	CAS Registry Number
54	Cloned(Commentary)
1	Crystallization (Commentary)
23	Disease/ Diagnostics
10	Expression
77	General Information
12	General Stability
241	Inhibitors
31	kcat/KM [mM/s]
3	Ki Value [mM]
148	KM Value [mM]
16	Localization
21	Metals/Ions
104	Molecular Weight [Da]
85	Natural Substrates/ Products (Substrates)
49	Organic Solvent Stability
572	Organism
10	Pathway
24	PDB ID
83	pH Optimum
28	pH Range
20	pH Stability
3	pI Value
5	Posttranslational Modification
147	Protein Variants
60	Purification (Commentary)
7	Reaction
5	Reaction Type
336	Reference
2	Renatured (Commentary)
44	Source Tissue
73	Specific Activity [micromol/min/mg]
3	Storage Stability
1017	Substrates and Products (Substrate)
108	Subunits
79	Synonyms
1	Systematic Name
73	Temperature Optimum [°C]
20	Temperature Range [°C]
59	Temperature Stability [°C]
66	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 3.5.5.1 - nitrilase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.33 - 0.47

2-chloromandelonitrile

2 entries

0.97 - 11.17

3-(4-chlorophenyl)pentanedinitrile

5 entries

0.001548 - 0.0032

3-aminobenzonitrile

3 entries

0.35 - 0.62

3-chloromandelonitrile

2 entries

0.001068 - 0.003

3-methylbenzonitrile

3 entries

0.001667

4-aminobenzonitrile

Rhodococcus rhodochrous

-

wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.36 - 0.5

4-chloromandelonitrile

2 entries

0.002867

4-methylbenzonitrile

Rhodococcus rhodochrous

-

wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.0011 - 205

Benzonitrile

4 entries

40.48

benzoylacetonitrile

Hoeflea phototrophica

-

pH 8.5, 50°C

755874

0.48

glutaronitrile

uncultured bacterium

E1A0Z9

pH 7.0, 30°C, recombinant His-tagged Nit1

710984

0.036 - 0.0363

iminodiacetonitrile

2 entries

2.33 - 17.38

mandelonitrile

3 entries

0.0079

Succinonitrile

uncultured Burkholderiaceae bacterium

A0A140D8I8

pH 7.2, 40°C

756289

0.33

2-chloromandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, wild-type enzyme

756207

0.47

2-chloromandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, mutant enzyme S190G

756207

0.97

3-(4-chlorophenyl)pentanedinitrile

Synechocystis sp. PCC 6803

Q55949

mutant P194A, sodium phosphate buffer, pH 7.0, 30°C

756359

1.69

3-(4-chlorophenyl)pentanedinitrile

Synechocystis sp. PCC 6803

Q55949

wild-type enzyme, sodium phosphate buffer, pH 7.0, 30°C

756359

5.96

3-(4-chlorophenyl)pentanedinitrile

Synechocystis sp. PCC 6803

Q55949

mutant F202V, sodium phosphate buffer, pH 7.0, 30°C

756359

11.02

3-(4-chlorophenyl)pentanedinitrile

Synechocystis sp. PCC 6803

Q55949

mutant P194A/F202V, sodium phosphate buffer, pH 7.0, 30°C

756359

11.17

3-(4-chlorophenyl)pentanedinitrile

Synechocystis sp. PCC 6803

Q55949

mutant P194A/I201A/F202V, sodium phosphate buffer, pH 7.0, 30°C

756359

0.001548

3-aminobenzonitrile

Rhodococcus rhodochrous

-

wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.00308

3-aminobenzonitrile

Rhodococcus rhodochrous

-

mutant enzyme R129K, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.0032

3-aminobenzonitrile

Rhodococcus rhodochrous

-

mutant enzyme R129H, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.35

3-chloromandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, wild-type enzyme

756207

0.62

3-chloromandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, mutant enzyme S190G

756207

0.001068

3-methylbenzonitrile

Rhodococcus rhodochrous

-

wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.0029

3-methylbenzonitrile

Rhodococcus rhodochrous

-

mutant enzyme R129K, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.003

3-methylbenzonitrile

Rhodococcus rhodochrous

-

mutant enzyme R129H, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.36

4-chloromandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, wild-type enzyme

756207

0.5

4-chloromandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, mutant enzyme S190G

756207

0.0011

Benzonitrile

Rhodococcus rhodochrous

-

mutant enzyme R129K, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.001223

Benzonitrile

Rhodococcus rhodochrous

-

wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

0.0015

Benzonitrile

Rhodococcus rhodochrous

-

mutant enzyme R129H, in 50 mM potassium phosphate (pH 7.5), at 25°C

712015

205

Benzonitrile

Pyrococcus sp. M24D13

-

pH 8.0, 80°C

756766

0.036

iminodiacetonitrile

Paenarthrobacter aurescens

S5M6X9

pH 7.2, 30°C

757343

0.0363

iminodiacetonitrile

Paenarthrobacter aurescens

S5M6X9

at pH 7.2 and 30°C

734397

2.33

mandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, wild-type enzyme

756207

8

mandelonitrile

Alcaligenes sp.

C5IIS9

at 30°C and pH 8.0

713444

17.38

mandelonitrile

uncultured organism

Q6RWE0

pH 7.5, 40°C, mutant enzyme S190G

756207