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3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Word Map on EC 3.5.5.1

Reaction

A nitrile
+ 2 H2O =
a carboxylate
 +
NH3

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase

Cloned

Cloned on EC 3.5.5.1 - nitrilase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a recombinant Escherichia coli BL21(DE3)/pET28a-cyc705 was constructed to achieve the heterologous expression of cyc705
cloned into expression vector pET-28a and expressed in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) under the control of T7 lac promoter using pET-28a to give pET-Nit, lactose is best as inducer in different media, method optimization
DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain BL21-Gold(DE3)/pOK101/pTf16, no cleavage of the C-terminal peptide of the enzyme in the recombinant bacteria
DNA and amino acid sequence determination, genes of the 3 isozymes are clustered on chromosome 3
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 CodonPlus (DE3) RIL cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli Rosetta-gami (DE3) cells
expressed in Escherichia coli strain BL21 (DE3) pLysS cells
-
expressed in Escherichia coli strain ER2566
-
expressed in Escherichia coli strain JM109
-
expressed in Escherichia coli strain JM109(pIK9)
-
expressed in Escherichia coli strains BL21 (DE3), BRLplys (DE3), Rosetta (DE3), and Tuner (DE3)
-
expressed, identified and displayed on the surface of Bacillus subtilis spores by using the spore coat protein G of Bacillus subtilis as an anchoring motif
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21(DE3)/pET28a-cyc705 with a recombinant plasmid pET28a-cyc705
-
expression in Escherichia coli strain BL21-Gold (DE3)
expression in Escherichia coli strain BL21-Gold (DE3), co-expression of GroEL/ES
expression in Rosetta 2(DE3)pLySs
expression into Escherichia coli BL21 (DE3)
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pSH
expression of the recombinant protein with His6-tag in Escherichia coli strain BL21
expression under the control of a rhamnose-inducible promoter in Escherichia coli strain JM109
functional expression in Escherichia coli strain BL21 (DE3)
gene nit1, DNA and amino acid sequence determination and analysis, expression of His-tagged Nit1 in Escherichia coli strain BL21(DE3)
isozyme expression analysis by quantitative by real-time PCR
-
overexpressed in Escherichia coli BL21 (DE3)
-
overexpression in Escherichia coli
-