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3.5.4.5: cytidine deaminase

This is an abbreviated version!
For detailed information about cytidine deaminase, go to the full flat file.

Word Map on EC 3.5.4.5

Reaction

2'-deoxycytidine
+
H2O
=
2'-deoxyuridine
+
NH3

Synonyms

activation-induced cytidine deaminase, AICDA, canine hepatic cyd deaminase, CDA, CDABcald, CDABpsy, CDABsub, CDase, CDD, CR deaminase, cyd deaminase, Cytidine aminohydrolase, cytidine deaminase, cytidine/2'-deoxycytidine aminohydrolase, cytosine nucleoside deaminase, DCD, dCDA, deoxycytidine deaminase, EC 3.5.4.14, mammalian deminase, T-CDA, yeast cytidine deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.5 cytidine deaminase

Purification

Purification on EC 3.5.4.5 - cytidine deaminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
nickel chelating affinity column chromatography
-
Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and butyl-Sepharose column chromatography
recombinant enzyme from Escherichia coli strain BL21 by two steps of anion exchange chromatography, and gel filtration
recombinant enzyme partially from 3T3 NIH and 3T3-CD3-V5 cells by ammonium sulfate fractionation
-
recombinant wild-type enzyme by affinity chromatography on a cytidine analog 6-[3,5(cytidyl) acryloylamino]hexanoic acid resin, recombinant inactive mutants by anion exchange chromatography, ultrafiltration, and gel filtration
-